Comparison of Corneal Preservation Media for Corneal Hydration and Stromal Proteoglycan Loss

Slack, James W.; Kangas, Tracy A.; Edelhauser, Henry F.; Geroski, Dayle H.; McDermott, Mark L.

doi:10.1097/00003226-199205000-00004

Cited by 18 publications

(7 citation statements)

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“…Thus, the present data suggest a progressive loss of approximately 1% of the total corneal content of KSPG immunoreactivity during the 28-day period. The finding is in accordance with a recent study where rabbit corneal proteoglycans were radiolabelled in situ and lost 1-2% of the labelled KSPG content during subsequent storage for 14 days at 4°C (Slack et al 1992). If such a KSPG leakage continues after grafting, it might partly explain the progressive corneal thinning seen in the first six months after a penetrating keratoplasty (Ehlers & Olsen 1983;Ehlers 1974).…”

Section: Discussionsupporting

confidence: 93%

Viability of human corneal keratocytes during organ culture

Møller‐Pedersen

Møller

1996

Acta Ophthalmologica Scandinavica

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The viability of human corneal keratocytes was assessed during four weeks of 'closed system' organ culture at 31 degrees C. After 28 days of culturing, the entire keratocyte population was still alive and viable because all cells incorporated uridine; a parameter for RNA-synthesis. During the first 14 days, mitoses were found in the anterior half of the stroma (0.23% mitoses per 48 h), while only few keratocytes were able to divide at day 28 (0.01% mitoses per 48 h). Metabolic parameters revealed a progressing acidosis in the medium with oxygen and glucose depletion. Immunological measurements of keratan sulphate proteoglycan suggested that approximately 1% of the total content was lost during the period. In conclusion, our current organ culture technique can maintain a viable keratocyte population for four weeks; a viable stroma can be grafted within this period.

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Section: Discussionsupporting

confidence: 93%

Viability of human corneal keratocytes during organ culture

Møller‐Pedersen

Møller

1996

Acta Ophthalmologica Scandinavica

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“…This was done on the basis that when the normal cornea swells, a small amount of fluid enters the fibrils but the majority of the swelling occurs between the fibrils [42]; the presence of direct interfibrillar cross-links would therefore be expected to restrict the ability of the cornea to swell. Cross-links formed between proteoglycan core proteins and collagen molecules (Figure 8E) would also be expected to alter the hydrodynamic behaviour of the cornea, by preventing some proteoglycans from leaching out of the corneal stroma during in vitro swelling [44]. Although Wollensak et al [20] have previously reported that cross-linked corneas swell less than normal their work was carried out by crosslinking the anterior cornea, which does not swell to any great extent and the riboflavin solution containing the deturgescent agent, dextran, was only applied to the UVA-treated corneas and not to their controls.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Riboflavin/UVA Collagen Cross-linking Therapy on the Structure and Hydrodynamic Behaviour of the Ungulate and Rabbit Corneal Stroma

et al. 2013

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PurposeTo examine the effect of riboflavin/UVA corneal crosslinking on stromal ultrastructure and hydrodynamic behaviour.MethodsOne hundred and seventeen enucleated ungulate eyes (112 pig and 5 sheep) and 3 pairs of rabbit eyes, with corneal epithelium removed, were divided into four treatment groups: Group 1 (28 pig, 2 sheep and 3 rabbits) were untreated; Group 2 (24 pig) were exposed to UVA light (3.04 mW/cm2) for 30 minutes and Group 3 (29 pig) and Group 4 (31 pig, 3 sheep and 3 rabbits) had riboflavin eye drops applied to the corneal surface every 5 minutes for 35 minutes. Five minutes after the initial riboflavin instillation, the corneas in Group 4 experienced a 30 minute exposure to UVA light (3.04 mW/cm2). X-ray scattering was used to obtain measurements of collagen interfibrillar spacing, spatial order, fibril diameter, D-periodicity and intermolecular spacing throughout the whole tissue thickness and as a function of tissue depth in the treated and untreated corneas. The effect of each treatment on the hydrodynamic behaviour of the cornea (its ability to swell in saline solution) and its resistance to enzymatic digestion were assessed using in vitro laboratory techniques.ResultsCorneal thickness decreased significantly following riboflavin application (p<0.01) and also to a lesser extent after UVA exposure (p<0.05). With the exception of the spatial order factor, which was higher in Group 4 than Group 1 (p<0.01), all other measured collagen parameters were unaltered by cross-linking, even within the most anterior 300 microns of the cornea. The cross-linking treatment had no effect on the hydrodynamic behaviour of the cornea but did cause a significant increase in its resistance to enzymatic digestion.ConclusionsIt seems likely that cross-links formed during riboflavin/UVA therapy occur predominantly at the collagen fibril surface and in the protein network surrounding the collagen.

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“…Corneas in organ culture are more edematous and typically preserved for longer than corneas in cold storage, and both of these factors have been associated with increased proteoglycan loss during preservation. 26 This proteoglycan loss during preservation might explain the relationship between thinner graftsin the early postoperative period and longer preservation times, 27 and might also result in reduced swelling pressure and therefore thinner corneas over the longer term. Regardless of the etiology, the difference in corneal thickness in the small study by Rijneveld et al was not associated with a difference in visual acuity.…”

Section: Discussionmentioning

confidence: 99%