Comparison of Cppu Effect on Fruit Development in Several Actinidia Species

Jo, Ye-Seul; Cho, H.S.; Park, M.Y.; Park, J.O.; Park, T.D.; Shim, Kyung Ku

doi:10.17660/actahortic.2003.610.72

Cited by 6 publications

(7 citation statements)

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“…To increase fruit set and to improve the yield of horticultural crops including pear, apple, grape and kiwifruit, CPPU has been systematically applied [25,26]. Interestingly, no significant effect of CPPU on controlling kiwifruit development has been reported in a few Actinidia species, such as A. macrosperma 'S7' [21]. In this study, we found both the size and weight of a single kiwifruit of A. chinensis 'Hongyang' strongly increased due to CPPU treatment (S1 Fig).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, no significant effect of CPPU on controlling kiwifruit development has been reported in a few Actinidia species, such as A . macrosperma ‘S7’ [ 21 ]. In this study, we found both the size and weight of a single kiwifruit of A .…”

Section: Discussionmentioning

confidence: 99%

“…Kiwifruit (genus Actinidia) is native to China, and is a rich nutritious fruit crop because of its high concentration of vitamins, minerals, and amino acids [18][19][20]. However, the characteristics of fruit shape, size, and flavor are distinct among different Actinidia species [21]. Interestingly, some species show impressive fruit performance (such as high vitamin C content, excellent flavor and sweetness), but their fruit weight is below 20 g, e.g., A. arguta, A. melanandra, A. macrosperma, and A. polygama [21].…”

Section: Introductionmentioning

confidence: 99%

“…However, the characteristics of fruit shape, size, and flavor are distinct among different Actinidia species [21]. Interestingly, some species show impressive fruit performance (such as high vitamin C content, excellent flavor and sweetness), but their fruit weight is below 20 g, e.g., A. arguta, A. melanandra, A. macrosperma, and A. polygama [21]. To increase the size and weight of kiwifruit, a synthetic cytokinin named N-(2-chloro-4-pyridyl)-N-phenylurea (CPPU) has been widely applied since the 1990s [22].…”

Section: Introductionmentioning

confidence: 99%

“…macrosperma , and A . polygama [ 21 ]. To increase the size and weight of kiwifruit, a synthetic cytokinin named N-(2-chloro-4-pyridyl)-N-phenylurea (CPPU) has been widely applied since the 1990s [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome sequencing and endogenous phytohormone analysis reveal new insights in CPPU controlling fruit development in kiwifruit (Actinidia chinensis)

Lan

Xiang

et al. 2020

PLoS ONE

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Kiwifruit (Actinidia chinensis) is a rich nutritious fruit crop owing to a markedly higher content of vitamin C and minerals. To promote fruit set and to increase the yield of kiwifruit, forchlorfenuron (CPPU) has been widely applied. However, the molecular details regarding CPPU controlling kiwifruit development, especially at the fastest fruit growth stage, remain unknown. In the present study, we measured the effect of CPPU on developmental regulation in red-fleshed kiwifruit (Actinidia chinensis 'Hongyang'). Additionally, a cytological analysis was performed to clarify the precise changes in the cell structure of the CPPU-treated kiwifruits. Moreover, the concentration of endogenous phytohormones, including indoleacetic acid (IAA), zeatin (ZT), gibberellic acid 3 (GA 3), and abscisic acid (ABA), were measured by Enzyme-linked Immunosorbent Assay (ELISA). Furthermore, RNA-Seq was performed to dissect the complicated molecular mechanisms, with a focus on biosynthesis, metabolism, and signaling compounds, such as endogenous hormones, sugars, and L-ascorbic acid. Our results demonstrated that CPPU treatment not only regulates the size and weight of a single fruit but also improves the quality in 'Hongyang' kiwifruit through the accumulation of both soluble sugar and vitamin C. It was also seen that CPPU regulates kiwifruit development by enhancing cell expansion of epidermal cells and parenchyma cells, while, promoting cell division of subepidermal cells. Additionally, CPPU significantly increased the gibberellin and cytokinin biosynthetic pathway and signaling, while repressing auxin and ABA biosynthetic pathway; thus, signaling plays an essential role in CPPU controlling kiwifruit development. Notably, transcriptomic analysis revealed that a total of 2244 genes, including 352 unannotated genes, were differentially expressed in kiwifruits because of CPPU treatment, including 127 transcription factors. These genes are mainly enriched in plant hormone signal transduction, photosynthesis, MAPK signaling pathway, starch and sucrose metabolism, and phenylpropanoid biosynthesis. Overall, our results highlight that CPPU regulation of kiwifruit development is mainly associated with an antagonistic and/or

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%