Comparison of creatine kinase M and B subunit, mRNAs and isoenzyme activity in ischemicdog myocardium

Metha, H.B.; Popovich, B.; Dillmann, Wolfgang H.

doi:10.1016/s0022-2828(87)80690-9

Cited by 4 publications

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“…This may be explained by the decrease in myocardium oxygen supply, whichinduces a fast decrease in M-subunit mRNA synthesis and an increase in the expression of B-subunit mRNA. 25 Otherwise, whatever the protocol, the drugs could have promoted an increase in heart work and consequently an increase in myocardium oxygen consumption as reported by Linde-Sipman et al 16 . Thus, this event could result in an increase in CK-MB serum activity as observed in the GI and GII dogs.…”

Section: Discussionmentioning

confidence: 90%

“…This parallel circulation system acts in response to coronary inflow decrease and leads to underestimationof the alteration of cardiac rhythm and serum enzyme activity, especially related to CK. 24 Even so, Mehta et al 25 reported that in the myocardium cells of dogs, short-duration anoxia induced a decrease in M-mRNA and an increase in the concentration of B-mRNA, resulting in a 35-100% elevation of CK-MB activity depending on duration of the ischemia.Experimental induction of myocardial ischemia in dogs has shown that partial or total occlusion of coronary branches significantly raises CK-MB serum activity. 26 Despite myocardium collateral circulation, several studies have reported cardiac alterations in dogs secondary to administration of anesthetic drugs.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of serum enzymatic markers of cardiomyocytes injury in female dogs submitted to ketamine S(+), atropin and xylazine association

Franco¹,

Fíoravantí²,

Damasceno³

et al. 2009

Acta Cir. Bras.

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Purpose: To assessment of the aspartate aminotransferase (AST), creatine kinase (CK) and creatine kinase isoenzyme fraction MB (CK-MB) serum activity in female dogs anesthetized with ketamine S (+), atropine and xylazine in several associations. Methods: Twenty three healthy female dogs randomly distributed in four groups named as GI (n=6), GII (n=6), GIII (n=6) and GIV (n=5) were treated respectively with atropine and ketamine S(+) (0.04mg/kg; 10 mg/kg); ketamine S(+) (10 mg/kg); atropine, xylazine and ketamine S(+) (0.04mg/kg; 1.1 mg/kg; 10 mg/kg) and xylazine and ketamine S(+) (1.1 mg/kg; 10 mg/kg). AST, CK and CK-MB serum activity measurement before pre-medication (M0) and one, two, three, six, 12, 24, 36 hours after. Results: There was no significant change in AST, CK e CK-MB serum activity among groups. However, CK serum activity in relation to moments within the groups was increased in all groups over the time in spite of treatment, except GI.In relation to CK-MB activity, in the moments within the group, it was observed an increase compared to baseline in all groups. Conclusion: Creatine kinase and creatine kinase fraction MB isoenzyme showed changes in their mean values remained higher than baseline for a longer time in GIII and GIV. Key words: Isoenzymes. Atropine. Xylazine. Heart. Ischemia. Anesthetics, Dissociative. Dogs. RESUMOObjetivo: Determinar a atividade sérica de AST, CK e CK-MB em cadelas anestesiadas com cetamina S (+), atropina e xilazina em diferentes associações. Métodos: Vinte e três cadelas saudáveis foram distribuídas ao acaso em quarto grupos denominados GI (n=6), GII (n=6), GIII (n=6) e GIV (n=5) tratados respectivamente com atropina e cetamina S (+) (0,04mg/kg; 10 mg/kg); cetamina S (+) (10 mg/kg); atropina, xilazina e cetamina S (+) (0,04mg/kg; 1,1 mg/kg; 10 mg/kg) exilazina e cetamina S (+) (1,1 mg/kg; 10 mg/kg). A atividade sérica de AST, CK e CK-MB foi determinada antes da pré-medicação (M0) e uma, duas, três seis, 12, 24 e 36 horas após M0. Resultados: Não foram encontradas mudanças significativas na atividade sérica de AST, CK e CK-MB entre grupos. Entretanto, entre momentos houve aumento da atividade sérica de CK para todos os grupos, exceto em GI.Com relação a atividade sérica de CK-MB, observou-se ao longo dos momentos aumento significativo com relação aos valores basais em ambos os grupos. Conclusão: Alterações significativas foram observadas com relação à atividade sérica de CK e CK-MB em todos os tratamentos, mantendo-se elevada por um período maior nos grupos GIII e GIV.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Assessment of serum enzymatic markers of cardiomyocytes injury in female dogs submitted to ketamine S(+), atropin and xylazine association

Franco¹,

Fíoravantí²,

Damasceno³

et al. 2009

Acta Cir. Bras.

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“…The control of the synthesis of CK is poorly understood. In myocardial cells of dogs, it was reported that short-duration anoxia induced a decrease in M-mRNA (up to 40% of initial content) and an increase in the concentration of B-mRNA, resulting in a 35-100% elevation of CK-MB activity according to the duration of the ischaemia (Mehta et aL, 1987(Mehta et aL, , 1988.…”

Section: Catalytic Activitymentioning

confidence: 99%

Creatine kinase in the dog: A review

et al. 1993

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In the dog, creatine kinase (CK) is mostly present in the skeletal muscles, myocardium, brain and intestine. The MM isoenzyme predominates in muscles and myocardium. In plasma, reference values depend on the technique used and CK-MB accounts for about 30-45% of total CK activity. Sex has no influence on plasma CK activity, which is higher in young dogs than in adults. Plasma CK is elevated after physical exercise. After its release from the cells, CK reaches the plasma mostly via the lymphatic route and then remains in the plasma compartment. It is rapidly cleared with a half-life of about 2 hours. Muscle diseases are the main source of plasma CK elevations: inherited myopathies, malignant hyperthermia, hypothyroidism, vitamin E-selenium deficiency, prolonged decubitus, intramuscular injections, surgery, etc. Plasma CK is also increased in experimental myocardial infarction, for which the dog is an interesting model, allowing quantification of the damage by measuring the total CK activity released.

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“…Comparable alterations have been described using a post-infarction left ventricular remodeling pig model (11). This hypothesis is supported by a study that demonstrated an acute increase in mRNA for CK-B in ischemic dog myocardium (12). Since CK gene expression is developmentally regulated, where proliferating myoblasts express the B subunit and differentiated muscle cells express the M subunit, it appears that myocardial ischemia induces an increase in the myocardial CK-B subunit mRNA.…”

Section: Ck-mbmentioning

confidence: 83%

The Specificity of Biochemical Markers of Cardiac Damage: a Problem Solved

Apple¹

1999

CCLM

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The UF-100 analyser is a fully automated instrument that stains the DNA and the membranes of the formed elements in native urine. The sample then passes as a laminar flow through a laser beam and light scattering, fluorescence and impedance are measured. The main purpose of the present work was to assess the analytical performance and the accuracy of the measurements of the UF-100 analyser. No carryover was observed, while the linearity was higher then the upper limit (40000 total particles microl(-1)) suggested by the manufacturer. The within-run imprecision was low, ranging from 17.7 % to 2.4% and was up to threefold better than manual microscopy. Comparison of results obtained by sediment microscopy (performed according to National Committee for Clinical Laboratory Standards (NCCLS) recommendations) and by the UF-100 analyser showed a linear correlation with r = 0.833 for erythrocytes, r = 0.934 for leukocytes, r = 0.880 for epithelial cells and r = 0.40 for casts. To evaluate the reliability of the UF-100 analyser in detecting bacteria we compared the results with the microbial culture (n = 608). Using a cut-off value of bacterial count above 1800 degrees l(-1) and at leukocyte count above 45 microl(-1), the analyser detected positive cultures with a sensitivity of 87 % and a specificity of 80 %. In conclusion, the UF-100 analyser can improve the work flow, increasing the output of urinalysis by reducing the number of specimens submitted for microscopy. Also the method provides reliable information for the identification of urinary tract inflammation and bacterial infection.

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Comparison of creatine kinase M and B subunit, mRNAs and isoenzyme activity in ischemicdog myocardium

Cited by 4 publications

References 0 publications

Assessment of serum enzymatic markers of cardiomyocytes injury in female dogs submitted to ketamine S(+), atropin and xylazine association

Assessment of serum enzymatic markers of cardiomyocytes injury in female dogs submitted to ketamine S(+), atropin and xylazine association

Creatine kinase in the dog: A review

The Specificity of Biochemical Markers of Cardiac Damage: a Problem Solved

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