Comparison of Cryopreservation Methods of Vegetatively Propagated Crops Based on Thermal Analysis

Zámečnı́k, Jiřı́; Faltus, Miloš; Bilavčík, Alois; Kotková, Renata

doi:10.5772/34454

Cited by 6 publications

(6 citation statements)

References 35 publications

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“…The reported devitrification event took place upon warming at 10ºC min -1 , twice as fast as our 5ºC min -1 slowest experiment (where ice formation would have been more likely). PVS3 was also previously reported to have a T G in good agreement with those reported here [28].…”

Section: Vitrification Behavior Of Different Vitrification Solutionssupporting

confidence: 91%

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Glass transition and heat capacity behaviors of plant vitrification solutions

et al. 2014

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Differential scanning calorimetry (DSC) was employed to investigate the vitrification and annealing behaviors of the most commonly used plant vitrification solutions (PVS). These solutions are employed to protect plant tissues towards ice formation and freeze injury, and help to the vitrification of these tissues, by globally reducing the intracellular fluids mobility. Glass transition temperatures (T g) and heat capacity increments (∆Cp) were determined for five solutions PVS1, PVS2, PVS2 mod, PVS3 and PVS3 mod, with different composition, and a range of cooling and warming rates was employed. Glass transitions showed clear and consistent temperature differences within vitrification solutions, which could be related to composition and water content. Roughly, two sets of T G values were obtained, those for PVS1 and 2, at-112 ºC and-114 ºC, respectively, and those for PSV3, at-90 ºC. The observed T g and ∆Cp, unexpectedly, did not significantly change within a wide range of cooling rates (from 5 ºC min-1 to liquid nitrogen quenching) and warming rates (from 5 to 20 ºC). Garlic shoot tips cryopreserved after the droplet method produced a similar result to that of the vitrification solutions employed. After quench cooling to temperatures below T g , repeated excursions to higher temperatures were made and the cooling and warming T g were recorded. These treatments had little or no effect over the PVS solutions T g , which remained practically constant. A direct practical consequence is that the plant vitrification solutions glass transition temperature does not significantly change with cryopreservation methods based on either direct plunging of samples into liquid nitrogen or employing closed cryovials.

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Section: Vitrification Behavior Of Different Vitrification Solutionssupporting

confidence: 91%

“…cryopreservation had been studied previously, using PVS2 [11] and PVS3 [28] cryopreservation solutions.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Glass transition and heat capacity behaviors of plant vitrification solutions

et al. 2014

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“…However, the stability of the amorphous state and the glass-forming ability of a number of concentrated and diluted aqueous PVSs have not yet been clarified. The development of cryogenic technologies is facilitated by biophysical studies capable of monitoring glass stability during cryopreservation (Zámečník et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Amorphous state stability of plant vitrification solutions

Vozovyk¹,

Bobrova²,

Prystalov³

et al. 2020

biologija

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Two critical factors for obtaining high viable biological samples after cryopreservation by vitrification method are cryoprotectant glass-forming ability while cooling and the stability of its amorphous state during warming. The present work is devoted to the study of the amorphous state stability of plant vitrification solutions (PVS) by differential scanning calorimetry method. The objects of the study were PVS1 modified, PVS2, PVS3 modified, PVS4 and PVSN. The thermograms of PVSs, frozen in liquid nitrogen, were recorded at a warming rate of 0.5 deg/min. The glass transition temperatures of PVS1, PVS2 and PVS3 were –109.0°C, –115.3°C, and –93.9°C, respectively. Neither exo- nor endothermic peaks have been recorded, indicating no crystallization, at the cooling and the warming stages. PVS4 and PVSN thermograms, besides glass transition (–111.5°C and –110.0°C, correspondingly), showed crystallization from the amorphous state (devitrification) and ice melting peaks. Crystallization enthalpy of PVSN was 2.8 times higher compared to PVS4. This fact testifies that the crystalline phase was larger in PVSN compared to PVS4. It should be noted that crystallization in PVS4 and PVSN may be related to the low warming rate used during the experiment. At higher warming rates, this crystallization is likely to be avoided. Thus, PVS1, PVS2 and PVS3 have a high glass-forming ability and a stabile amorphous state. The amorphous state stability of PVS4 and PVSN is significantly lower compared to PVS1, PVS2, and PVS3.

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“…The basic characteristics of substances used in cryopreservation are summarized in Table 1. The glass transition temperature tends to increase with increasing the relative molecular mass as opposed to the melting point [33]. In this order, the most commonly used cryoprotective substances are glycerol (Gly), dimethyl sulfoxide (DMSO), ethylene glycol (EG), propylene glycol (PG), sucrose (Suc), and sorbitol (Sor), at different concentrations in combination with MS medium [34] to allow sufficient dehydration of plant material and also induce cryoprotective processes to the cells [35].…”

Section: Cryoprotective Substancesmentioning

confidence: 99%

“…The development of cryogenic technologies is facilitated by biophysical studies capable of monitoring glass stability during cryopreservation [33]. The glass transition temperature of substances depends on the concentration of an aqueous solution, cooling/warming rates, annealing temperature, and type of mixture.…”

Section: Substances That Do Not Penetrate Through the Cell Wallmentioning

confidence: 99%

Vitrification Solutions for Plant Cryopreservation: Modification and Properties

Zámečnı́k

Faltus

Bilavčík

2021

Plants

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Many plants cannot vitrify themselves because they lack glassy state-inducing substances and/or have high water content. Therefore, cryoprotectants are used to induce vitrification. A cryoprotectant must have at least the following primary abilities: high glass-forming property, dehydration strength on a colligative basis to dehydrate plant cells to induce the vitrification state, and must not be toxic for plants. This review introduces the compounds used for vitrification solutions (VSs), their properties indicating a modification of different plant vitrification solutions, their modifications in the compounds, and/or their concentration. An experimental comparison is listed based on the survival or regeneration rate of one particular species after using more than three different VSs or their modifications. A brief overview of various cryopreservation methods using the Plant Vitrification Solution (PVS) is also included. This review can help in alert researchers to newly introduced PVSs for plant vitrification cryoprotocols, their properties, and the choice of their modifications in the compounds and/or their concentration.

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Comparison of Cryopreservation Methods of Vegetatively Propagated Crops Based on Thermal Analysis

Cited by 6 publications

References 35 publications

Glass transition and heat capacity behaviors of plant vitrification solutions

Glass transition and heat capacity behaviors of plant vitrification solutions

Amorphous state stability of plant vitrification solutions

Vitrification Solutions for Plant Cryopreservation: Modification and Properties

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