Comparison of Cryotop and ReproCarreir products for cryopreservation of bovine blastocysts through survival rate and blastocysts quality

Abstract

Cryopreservation of oocytes and embryos in assisted reproductive technology for application in domestic animals and humans is very important to preserve the maternal and paternal genetic complements. Cryopreservation allows widespread use of valuable animal embryos to improve the chances of pregnancy. However, intracellular ice formation in cryopreservation methods for oocytes and embryos can lead to fatal damage. To overcome this issue of intracellular ice formation, slow freezing and vitrification methods are most widely used (Mucci et al., 2006).The balance of redox reactions in cells is very crucial for maintaining the metabolic environment and gene expression during embryonic development. However, high levels of reactive oxygen species (ROS) were seen in preimplantation embryos of cattle (Min et al., 2014). ROS, such as hydrogen peroxide, cause oxidative stress, which is known to cause DNA damage and apoptosis (Takahashi, 2012). Therefore, regulating oxidative stress and ROS production during the vitrification-warming process is criti-

The antioxidant capacity of Mito-TEMPO improves the preimplantation development and viability of vitrified-warmed blastocysts through the stabilization of F-actin morphological aspects in bovine embryos

“…Cryopreservation was performed using a Cryotop (Kitazato Supply Co, Fujinomiya, Japan) for vitrification via a slightly modified procedure previously described by Kim et al 2020(Kim et al, 2020. Briefly, two or three…”

Effects of Mito-TEMPO on the survival of vitrified bovine blastocysts in vitro

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Cryopreservation as a technique for reproduction is used to preserve the genetic complements on domestic animals and humans (Lee et al, 2014;Kim et al, 2020).Especially, cryopreservation technology provides ad-ditional opportunity of pregnancy through the vitrifiedwarmed process in animal embryos. This is applied as a necessary technique for pregnancy using in vitro fertilization (IVF) embryos to solve the difficulty of female infertility (Hara et al, 2018).

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“…Cryopreservation as a technique for reproduction is used to preserve the genetic complements on domestic animals and humans (Lee et al, 2014;Kim et al, 2020).…”