1993
DOI: 10.1155/1994/804205
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Comparison of Culture, Cytotoxin Assay and Two Eia Tests with Clinical Diagnosis of Clostridium difficile‐Associated Diarrhea

Abstract: The sensitivity and specificity of eia for toxin A is similar to other tests. However, due to rapidity and ease of performance, it may be a more practical test for the diagnosis of C difficile-associated diarrhea, especially if the cytotoxin assay is not available.

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“…The cytopathic effect is characterised by rounding and morphologic changes of the cultured cells[ 29 , 30 ], and the phenomena must also be reverted by C. difficile or Clostridium sordellii anti-toxin used as a control to prove that the cytopathic effect is not related to nonspecific substances in the faeces. In contrast, toxigenic culture requires faeces inoculation to selective cycloserine-cefoxitin-fructose, chromogenic or similar agar plates for a specific incubation period; suspicious colonies are selected for further bacteria culture and/or C. difficile toxin test to confirm the finding (Figure 3 )[ 31 - 33 ]. It is important to remember that there is no standard to eliminate or reduce nonspecific bacteria colonisation from C. difficile culture.…”
Section: Current Diagnostic Tools For Identifying Patients With ...mentioning
confidence: 99%
“…The cytopathic effect is characterised by rounding and morphologic changes of the cultured cells[ 29 , 30 ], and the phenomena must also be reverted by C. difficile or Clostridium sordellii anti-toxin used as a control to prove that the cytopathic effect is not related to nonspecific substances in the faeces. In contrast, toxigenic culture requires faeces inoculation to selective cycloserine-cefoxitin-fructose, chromogenic or similar agar plates for a specific incubation period; suspicious colonies are selected for further bacteria culture and/or C. difficile toxin test to confirm the finding (Figure 3 )[ 31 - 33 ]. It is important to remember that there is no standard to eliminate or reduce nonspecific bacteria colonisation from C. difficile culture.…”
Section: Current Diagnostic Tools For Identifying Patients With ...mentioning
confidence: 99%