Comparison of Culture Media for Bile Acid Transport Studies in Primary Human Hepatocytes

Abstract

Williams' medium E is superior to DMEM for transport studies in primary human hepatocytes. Supplementation with dexamethasone increase mRNA levels of NTCP and BSEP.

Addition of Dexamethasone Alters the Bile Acid Composition by Inducing CYP8B1 in Primary Cultures of Human Hepatocytes

“…20,21 Since dexamethasone is a common additive to culture media, we have previously investigated their effect on bile acid transporters in primary hepatocytes and shown that dexamethasone addition in the cell culture media increased the expression of BSEP and NTCP. 22 In this study, we investigated how different substrates, collagen and matrigel, and the addition of dexamethasone to the culture media influence bile acid synthesis and secretion.…”

Liver safety evaluation of endothelin receptor antagonists using HepatoPac®: A single model impact assessment on hepatocellular health, function and bile acid disposition

“…In addition, while BSEP inhibition in vesicles and hepatocellular transport for CLF give insights into the potential of a drug to affect the overall bile acid transport, the ability to separate compound effects specific to uptake (CL biliary ) and efflux (%BEI) is an important factor in assessing potential risk. However, toxic effects due to the build‐up of cytotoxic bile acids may not be adequately present in in vitro model systems (Mork et al, ) and other investigators have added bile acids to the culture media to circumvent this potential limitation (Chatterjee, Richert, Augustijns, & Annaert, ; Susukida, Sekine, Nozaki, Tokizono, & Ito, ). While the ability to observe these endpoints in a single model offers consistency that is not available across multiple test systems, a missing component from this model is an endpoint specifically related to mitochondrial dysfunction.…”

Rusip Sehubungan Profil Lipid Dalam Keadaan Hiperkolesterolemia

“…Pemberian rusip di tikus hiperkolesterolemi selama 14 hari secara bermakna berhasil menurunkan kadar jumlah keseluruhan serum kolesterol; hal ini sesuai dengan beberapa telitian yang menyatakan bahwa penambahan makanan fermentasi bakteri asam laktat dan kalsium dapat menurunkan kolesterol dalam serum tikus hiperkolesterolemi. [9][10][11]19,[22][23][24] Hasil telitian menunjukkan penurunan kadar jumlah keseluruhan kolesterol, karena pemberian rusip selama 14 hari di tikus percobaan tidak mencapai kadar yang normal atau kolesterol awal. Hal ini mungkin karena diperlukan waktu lebih lama untuk menurunkan kadar kolesterol menjadi seperti semula.…”

“…Mekanisme lain adalah penurunan kadar serum kolesterol oleh bakteri asam laktat melalui dekonjugasi garam empedu. 10,22,23 Kedua mekanisme penurunan serum kolesterol di atas melibatkan bakteri asam laktat. Beberapa jurnal terbaru menyebutkan, bahwa penambahan kalsium dalam jumlah tertentu dapat membantu menurunkan kadar serum kolesterol.…”

“…Garam empedu primer yang seharusnya digunakan sebagai pengemulsi lipid dan berperan dalam penyerapannya, didekonjugasi oleh bakteri asam laktat mejadi garam empedu sekunder yang tingkat kelarutan dalam lumen jauh lebih rendah. 9,10,22,23 Hal ini menyebabkan garam empedu tidak mengemulsikan lipid dan tidak diserap oleh lumen usus. Garam empedu dan lipid yang tidak teremulsikan akan keluar bersama feses, sehingga banyaknya garam empedu yang hilang menyebabkan cadangan di kantung empedu sedikit.…”