Comparison of culture method and real-time PCR for detection of putative periodontopathogenic bacteria in deep periodontal pockets

Kotsilkov, Kamen; Popova, Chrisitina; Boyanova, Lyudmila; Setchanova, Lena; Mitov, Ivan

doi:10.1080/13102818.2015.1058188

Cited by 32 publications

(42 citation statements)

References 22 publications

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“…Among the periodontal bacteria, the red complex group; P.gingivalis, T. forsythia, and T. denticola have been strongly implicated as chief pathogens of periodontitis, and the strong correlation between the presences of these bacteria and mixed periodontal infections was proved. These organisms are powerful contributors in the development of adult, chronic periodontitis and also oral malodor [9,10].…”

Section: Discussionmentioning

confidence: 99%

Colorimetric LAMP Molecular Method for Immediate Detection of Three Periodontal Pathogens

Al-Hamdoni¹,

Al-Rawi²

2020

Preprint

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Background and Purpose: For enhancing the efficiency of diagnostic tools and overcome drawbacks faced in diagnosis attempt, molecular methods have been developed aid accurate and rapid identification of bacterial species particularly those which are difficult to study with. The current study searched to find a more specific and rapid method serves researchers for the identification of periodontal pathogens and overcome disturbances encountered in study such anaerobic fastidious organisms. Methods and Results: Three periodontal pathogens, Porphyromonas gingivalis , Tannerella forsythia and Treponema denticola were characterized and identified by phenotypic features and Loop- Mediated Isothermal Amplification (LAMP) techniques which involved 4 sets of primers targeted to 16SrRNA genes and loop primers and the Colorimetric Master Mix containing Bst DNA polymerase and Phenol Red for the detection of amplicon formation. There was a variance in phenotypic characters of the isolates of the same species. LAMP, as a novel molecular technique was of usefulness value in identifying the target weather as extracted DNA or whole cells in a high specific and very rapid manner within 30 min. by visual reading of the results. Conclusion: It is strongly recommended the use of the novel LAMP method in researches work with periodontal pathogens as its advantages make it superior to other molecular techniques in respects of a higher specificity, rapidity, sensitivity and overcome DNA extraction step and special equipment in that it can be used at chair- side identification.

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Section: Discussionmentioning

confidence: 99%

Colorimetric LAMP Molecular Method for Immediate Detection of Three Periodontal Pathogens

Al-Hamdoni¹,

Al-Rawi²

2020

Preprint

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“…Postoje različite metode koje se primjenjuju za kvantifikaciju periimplantantnog i parodontnog mikrobioma i njihove diferencijacije, ali još uvijek nema rutinski dostupnog i standardiziranog postupka kojim se može procijeniti stvarno bakterijsko opterećenje u različitim fazama svakoga od tih patoloških stanja (13,14).…”

Section: Methodsunclassified

“…There are various methods used for quantification purpose of peri-implant and periodontal microbiomes and their differentiation, but question of routinely accessible and standardized approach that can estimate actual bacterial load at the different stages of each of these pathologies remains not fully resolved (13,14).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Bacterial Load Parameters in Subgingival Plaque during Peri-implantitis and Periodontitis Using the RT-PCR Method

Nastych

Goncharuk-Khomyn

Foros

et al. 2020

Acta Stomatol Croat

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Iako su parodontitis i periimplantitis uzročno povezani s takozvanim parodontopatogenim bakterijama, rezultati nedavno objavljenog sistematiziranog preglednog rada i metaanaliza dali su visokokvalitetne dokaze da obrazac bolesti određuju uglavnom kvantitativne karakteristike oralnoga mikrobioma, a ne samo određeni patogen (1, 2, 3, 4). Čini se da je mikrobiom kvalitativno razmjerno sličan u zdravom i bolesnom parodontu ili oko implantata, ali su uočene promje-ACTA STOMATOLOGICA CROATICA

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“…The comparison of both methods revealed a better diagnostic capability and sensitivity of the PCR (32) .…”

mentioning

confidence: 98%

“…This microbiological culture method was one of the most widely used techniques for microbiological detection. However, this method has some drawbacks that limit its application in our study as it has a very low sensitivity, specifity and it is especially difficult to cultivate anaerobic species which are considered species within point of research where microorganisms related to the periodontal disease, in turn, are predominantly anaerobic and are very difficult to cultivate (32) .…”

mentioning

confidence: 99%

Evaluation of Topical Subgingival Application of Hyaluronic Acid (Ha) Gel Adjunctive to Scaling and Root Planing (Srp) in the Treatment of Chronic Periodontitis.

Kandil¹,

Khashaba²,

El-Daker³

et al. 2017

IJAR

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Objective: The aim of this study is to assess effect of sub-gingival application of hyaluronic acid (HA) gel .8% adjunctive to non-mechanical debridement in treatment of chronic periodontitis based on clinical scoring and microbiological study. Subjects and Methods: The present study was carried out on 20 patients with chronic periodontitis with at least two quadrants with pocket depth ≥5 mm within the same arch. Split-mouth study design was used where control side received (SRP) only and study side received both (SRP) and subgingival application of high molecular weight 0.8% hyaluronic acid gel in a 3 months follow-up study. After clinical assessment and plaque sample collection all patients received full mouth SRP in both study and control sites at the baseline, 2 nd , 4 th and 6 th weeks. Study sites additionally received 0.8% HMW HA gel sub-gingivally till the depth of pocket, this done once a week starting at the baseline then repeated at weeks 1, 2, 3, 4, 5 and 6 for a total seven applications. Subgingival plaque samples were collected prior to clinical measurements from both study and control sites at the baseline, 6 th and 12 th weeks for PCR examination. Evaluation included clinical and microbiological parameters that recorded at the base line, th 6 th and then in 12 th week. Clinical parameters included: Plaque index (PI), Gingival index(GI), Periodontal probing depths (PPD), Clinical attachment loss (CAL) and clinical attachment regain where, micriobiological assessment included qualitative polymerase chain reaction (PCR) for detection of both provotella intermedia and porphyromonus gingivalis in both sides . Results: In the present study, there were highly significant clinical improvements in all measured clinical

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Comparison of culture method and real-time PCR for detection of putative periodontopathogenic bacteria in deep periodontal pockets

Cited by 32 publications

References 22 publications

Colorimetric LAMP Molecular Method for Immediate Detection of Three Periodontal Pathogens

Colorimetric LAMP Molecular Method for Immediate Detection of Three Periodontal Pathogens

Comparison of Bacterial Load Parameters in Subgingival Plaque during Peri-implantitis and Periodontitis Using the RT-PCR Method

Evaluation of Topical Subgingival Application of Hyaluronic Acid (Ha) Gel Adjunctive to Scaling and Root Planing (Srp) in the Treatment of Chronic Periodontitis.

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