Comparison of Cytotoxicity and the Anti-Adipogenic Effect of Green Tea Polyphenols with Epigallocatechin-3-Gallate in 3T3-L1 Preadipocytes

Lao, Weiguo; Tan, Yi; Jin, Xiaoli; Xiao, Linda; Kim, Jane J Y; Qu, Xianqin

doi:10.1142/s0192415x15500676

Cited by 24 publications

(21 citation statements)

References 19 publications

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“…In addition, a novel acylated flavonol tetraglycoside from Lu an GuaPian tea (a kind of green tea) remarkably inhibited the proliferation, differentiation, and lipid accumulation of 3T3-l1 cells [33]. Total green tea polyphenols might exhibit a greater inhibitory effect than purified EGCG on adipogenesis in 3T3-l1 cells, through decreasing adipogenic factors, including CCAAT element-binding protein α, peroxisome proliferator-activated receptor γ (PPAR γ), and sterol regulatory element-binding protein-1c (SREBP-1c) [155].…”

Section: Anti-obesity Effect In Vitromentioning

confidence: 99%

Health Functions and Related Molecular Mechanisms of Tea Components: An Update Review

Tang

Meng

Gan

et al. 2019

IJMS

270

150

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Tea is widely consumed all over the world. Generally, tea is divided into six categories: White, green, yellow, oolong, black, and dark teas, based on the fermentation degree. Tea contains abundant phytochemicals, such as polyphenols, pigments, polysaccharides, alkaloids, free amino acids, and saponins. However, the bioavailability of tea phytochemicals is relatively low. Thus, some novel technologies like nanotechnology have been developed to improve the bioavailability of tea bioactive components and consequently enhance the bioactivity. So far, many studies have demonstrated that tea shows various health functions, such as antioxidant, anti-inflammatory, immuno-regulatory, anticancer, cardiovascular-protective, anti-diabetic, anti-obesity, and hepato-protective effects. Moreover, it is also considered that drinking tea is safe to humans, since reports about the severe adverse effects of tea consumption are rare. In order to provide a better understanding of tea and its health potential, this review summarizes and discusses recent literature on the bioactive components, bioavailability, health functions, and safety issues of tea, with special attention paid to the related molecular mechanisms of tea health functions.

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Section: Anti-obesity Effect In Vitromentioning

confidence: 99%

Health Functions and Related Molecular Mechanisms of Tea Components: An Update Review

Tang

Meng

Gan

et al. 2019

IJMS

270

150

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“…The mouse adipocyte 3T3-L1 cell line has been extensively used to study the in vitro effect of tea extracts and its isolated compounds [71][72][73]. In obesity in vivo studies, C57BL/6J mice are the most widely animal model since they are susceptible to high fat diet-induced obesity [74,75].…”

Section: Camellia Sinensis and Obesitymentioning

confidence: 99%

“…Moreover, the mitogen activated protein kinases (MAPKs) signaling pathways (extracellular signal regulated kinases (ERKs), Jun amino terminal kinases (JNKs), and stress activated protein kinases (p38/SAPKs)) are involved in adipocyte differentiation and in adipogenesis regulation [79]. Green tea polyphenols, gallocatechin gallate, and epigallocatechin-3-gallate decreased MAPK pathway activation as evidenced in downregulation of adipogenic factor expression (CCAAT element binding protein α (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ), and sterol regulatory element-binding protein-1c (SREBP-1c)) in a differentiated mouse adipocyte 3T3-L1 cell line [72,73]. Furthermore, the compound gallocatechin gallate has also demonstrated inhibition of inflammation through NF-κB signaling; adipose tissue inflammation is involved in several pathologies associated with obesity such as diabetes mellitus type 2 [73].…”

Section: Camellia Sinensis and Obesitymentioning

confidence: 99%

The Pharmacological Activity of Camellia sinensis (L.) Kuntze on Metabolic and Endocrine Disorders: A Systematic Review

et al. 2020

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Tea made from Camellia sinensis leaves is one of the most consumed beverages worldwide. This systematic review aims to update Camellia sinensis pharmacological activity on metabolic and endocrine disorders. Inclusion criteria were preclinical and clinical studies of tea extracts and isolated compounds on osteoporosis, hypertension, diabetes, metabolic syndrome, hypercholesterolemia, and obesity written in English between 2014 and 2019 and published in Pubmed, Science Direct, and Scopus. From a total of 1384 studies, 80 reports met inclusion criteria. Most papers were published in 2015 (29.3%) and 2017 (20.6%), conducted in China (28.75%), US (12.5%), and South Korea (10%) and carried out with extracts (67.5%, especially green tea) and isolated compounds (41.25%, especially epigallocatechin gallate). Most pharmacological studies were in vitro and in vivo studies focused on diabetes and obesity. Clinical trials, although they have demonstrated promising results, are very limited. Future research should be aimed at providing more clinical evidence on less studied pathologies such as osteoporosis, hypertension, and metabolic syndrome. Given the close relationship among all endocrine disorders, it would be of interest to find a standard dose of tea or their bioactive constituents that would be beneficial for all of them.

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“…This adipogenic medium was refreshed every 2 days. The numbers of mature adipocytes was determined by visual inspection of the lipid droplet formation under light microscopy (Olympus, BX51 microscope, Tokyo, Japan) followed by Oil Red O staining (Sigma-Aldrich, St Louis, MO, USA) and triglycerides measurement described previously [11] on day 7, 14, 21 and 28 after induction treatment. Triglyceride contents were increased more than 10 times on day 21 of adipogenic induction.…”

Section: Hadscs Differentiation and Treatmentmentioning

confidence: 99%

“…Our previous studies showed that GTP exert anti-adipogenic effects on preadipocyte proliferation in 3T3-L1 adipocytes [11] and ameliorate metabolic abnormalities and insulin resistance in obese Zucker fatty rats [12]. In the present study we evaluated whether GTP is capable of modulating the differentiation of human adipose tissue-derived stem cells (hADSCs) shifting towards osteogenic lineage and suppressing adipogenesis during early stage of differentiation.…”

Section: Introductionmentioning

confidence: 98%

Combating Osteoporosis and Obesity With Green Tea Polyphenols: Molecular Switching of Osteogenesis Versus Adipogenesis During Early Differentiation of Human Adipose Tissue-Derived Stem Cells

Lao¹,

Tan²,

Johnson³

et al. 2020

Preprint

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Background: Osteoporosis is a metabolic disease affecting the bone mineral density associated with increased adiposity in the aging population with obesity. The nutrients to control osteoblast and adipocyte differentiation from a common precursor, the pluripotent mesenchymal stem cell (MSC), may be a promising therapy for osteoporosis. Previously, we have shown that green tea polyphenols (GTP) exert anti-adipogenic effects on preadipocyte proliferation. In the present study, we investigated regulatory effects of GTP on osteogenesis and adipogenesis during early differentiation of human adipose tissue-derived stem cells (hADSCs). Methods: GTP at concentrations of 1 and 10 µg/ml was incubated with primary hADSCs in presence or absence of pioglitazone (100 µmol) during hADSCs differentiation. Adipogenesis of hADSCs was determined by Oil Red O staining and measurement of the cellular triglyceride synthesis in mature adipocyte. Alkaline phosphatase (ALP) assay and the measurement of intracellular calcium were utilized to determine osteoporosis of hADSCs. Immunofluorescence staining and qRT-PCR were employed to detect PPARγ-CEBPA regulated adipogenic pathway and the RUNX2-BMP2 mediated osteogenic pathway. Results: GTP treatment significantly decreased lipid accumulation and the cellular triglyceride synthesis in mature adipocytes and attenuated pioglitazone-induced adipogenesis in a dose-dependent manner. GTP downregulated protein and mRNA expression of Pparγ and attenuated pioglitazone-stimulated Cebpa expression in mature adipocytes. Concurrently, measurements of calcium content and ALP activity showed that GTP treatment significantly enhanced hADSCs differentiation into osteocytes compared with the control and pioglitazone-treated cells. Meanwhile, GTP upregulated protein and mRNA expression of RunX2 and Bmp2 compared to the control and GTP at 10 µg/mL significantly attenuated the decreased mRNA expression of Runx2 and Bmp2 by pioglitazone. Conclusions: The present study demonstrated that GTP possess a greater ability to facilitate osteogenesis and simultaneously inhibit hADSCs differentiation into the adipogenic lineage through upregulating the RUNX2-BMP2 mediated osteogenic pathway and suppressing PPARγ-induced signaling of adipogenesis. The findings of this study highlight that GTP may be a therapeutic intervention to combat osteoporosis associated obesity.

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Comparison of Cytotoxicity and the Anti-Adipogenic Effect of Green Tea Polyphenols with Epigallocatechin-3-Gallate in 3T3-L1 Preadipocytes

Cited by 24 publications

References 19 publications

Health Functions and Related Molecular Mechanisms of Tea Components: An Update Review

Health Functions and Related Molecular Mechanisms of Tea Components: An Update Review

The Pharmacological Activity of Camellia sinensis (L.) Kuntze on Metabolic and Endocrine Disorders: A Systematic Review

Combating Osteoporosis and Obesity With Green Tea Polyphenols: Molecular Switching of Osteogenesis Versus Adipogenesis During Early Differentiation of Human Adipose Tissue-Derived Stem Cells

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