Comparison of detection speed and yield in agitated and non‐agitated aerobic blood culture bottles

Arpi, Magnus; Larsen, Severin Olesen

doi:10.1111/j.1699-0463.1992.tb04041.x

Cited by 11 publications

(6 citation statements)

References 16 publications

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“…In our opinion the current data confirm that a beta-lactam-aminoglycoside combination is a valid first choice for patients in our region suspected of bacteraemia, in accordance with a previous survey covering 1978-1983 (9). Antistaphylococcal coverage by e.g.…”

Section: Discussionsupporting

confidence: 92%

The impact of the first notification of positive blood cultures on antibiotic therapy

Schønheyder

Højbjerg

1995

APMIS

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regimen being ampicillin or an ampicillin-aminoglycoside combination (295 episodes), whereas cephalosporins, thienamycin, and fluoroquinolones were seldom used (41 episodes). The ongoing antibiotic coverage was deemed appropriate in 41 8 episodes (60%), non-optimal in 90 (13%), and lacking in 186 (27%). The notification of positive blood cultures elicited changes in antibiotic therapy in 315 episodes (45%), including commencement of antibiotic therapy in 127 (18%). Thus, blood culture results have a measurable impact on antibiotic therapy.

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Section: Discussionsupporting

confidence: 92%

The impact of the first notification of positive blood cultures on antibiotic therapy

Schønheyder

Højbjerg

1995

APMIS

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“…Indeed, it is commonplace to find that the caregivers can’t afford to pay for blood cultures before commencement of antibiotics. The use of manual culture techniques which accounted for 89% of techniques used in this review may have greatly hampered detection of GBS and other pathogens for the following reasons: automated blood culture systems support the growth of a wider range of organisms and at lower inoculum than manual systems, they also have antibiotic removal devices such as resins which help to enhance microbial growth in the presence of antibiotics, the continuous agitation of bottles by the equipment also encourages bacterial growth [ 55 , 56 ]. Dagnew et al reported higher isolation of GBS with automated systems[ 4 ].…”

Section: Discussionmentioning

confidence: 99%

Aetiology of neonatal sepsis in Nigeria, and relevance of Group b streptococcus: A systematic review

et al. 2018

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BackgroundGroup B Streptococcus (GBS) causes invasive infections in neonates and has been implicated as a cause of prelabour rupture of membranes, preterm delivery and stillbirths. The success of phase II trials of polyvalent polysaccharide GBS vaccines indicates that these infections are potentially preventable. Nigeria is the most populous country in Africa with one of the highest birth rates, one of the highest neonatal sepsis incidence rates and one of the highest mortality rates in the world. Therefore, before the possible introduction of preventive strategies such as intrapartum antibiotic prophylaxis or GBS vaccine into Nigeria, it is vital that there is accurate data on the aetiology of neonatal sepsis and on the incidence of GBS neonatal sepsis in particular. The objective of this study was to determine the incidence and aetiology of neonatal sepsis in Nigeria with a focus on GBS sepsis and also to assess the potential impact of a GBS vaccine.MethodsA literature search was conducted on the databases of African journals online, PubMed and Google Scholar for works conducted between 1987 to 2017. Case reports, reviews, and studies not stating specific culture methods or specific bacteria isolated were excluded. Data extracted included; incidence of neonatal sepsis, method of blood culture, blood volume, sample size, bacterial agents isolated and history of antibiotic use. PRISMA guidelines were followed and modified Down’s and Black criteria used to evaluate the quality of studies.ResultsA total of 5,114 studies were reviewed for neonatal sepsis out of which 24 consisting of a total of 2,280 cases were selected for final review. Nine studies met criteria for assessment of hospital based incidence of neonatal sepsis representing 31,305 hospital births. The incidence of neonatal sepsis was 18.2/1000 livebirths with range from 7-55/1000 livebirths while the GBS incidence was 0.06/1000 livebirths with range from 0-2/1000 live births. We discovered various limitations such as identification techniques that could result in underestimation of the true incidence of GBS sepsis. Pathogens such as Klebsiella pneumoniae and Staphylococcus aureus were more commonly isolated than GBS.Implications of key findingsThe hospital based incidence of neonatal sepsis was high at 18.2/1000 live births while that due to GBS was 0.06/1000 live births. The burden of neonatal sepsis, including that attributable to GBS is substantial and could be reduced by preventive strategies such as intrapartum antibiotic prophylaxis or GBS vaccine. There is however very sparse meaningful data currently. Well planned prospective studies with larger sample sizes, more advanced isolation and identification techniques and those following up invasive disease cases for possible short and long term sequelae are needed—not only prior to possible introduction of the vaccine to determine the baseline epidemiology, but also thereafter to monitor its impact on the population. Strategies need to be developed to also reduce the morbidity and mortality attributa...

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“…Blood culture automates agitate the blood cultures continuously during incubation, which has been shown to increase recovery of pathogens, probably due to increased oxygenation. [42][43][44][45][46] Moreover, temperature in automated incubators is presumably more stable than that in a conventional incubator, which has to be opened in its entirety each time a bottle is added or removed.…”

Section: Discussionmentioning

confidence: 99%

Nonautomated Blood Cultures in a Low-Resource Setting: Optimizing the Timing of Blind Subculture

Ombelet

Peeters

Phe

et al. 2021

The American Journal of Tropical Medicine and Hygiene

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Laboratory procedures for blood cultures in a hospital in Phnom Penh were adapted to optimize detection of Burkholderia pseudomallei, an important pathogen in this setting. The effects of these changes are analyzed in this study. Blood cultures consisted of two BacT/ALERT bottles (bioMérieux, Marcy-l'Etoile, France). Growth was detected visually by daily inspection of the bottles. In 2016, the aerobic-anaerobic pair (FA/FN FAN) was substituted by an aerobic pair of BacT/ALERT FA Plus bottles. Blind subculture (BS) (subculture in the absence of visual growth) was advanced from day 3 to day 2 of incubation in July 2016. In July 2018, it was further advanced to day 1 of incubation. From July 2016 to October 2019, 9,760 blood cultures were sampled. The proportion of cultures showing pathogen growth decreased from 9.6% to 6.8% after the implementation of the laboratory changes (P < 0.001). Advancing the BS from day 3 to day 2 led to an increased proportion of pathogens detected by day 3 (92.8% versus 82.3%; P < 0.001); for B. pseudomallei, this increase was even more remarkable (92.0% versus 18.2%). Blind subculture on day 1 similarly increased the proportion of pathogens detected by day 2 (82.9% versus 69.0% overall, 66.7% versus 10.0% for B. pseudomallei; both P < 0.001). However, after implementation of day 1 subculture, a decrease in recovery of B. pseudomallei was observed (12.4% of all pathogens versus 4.3%; P < 0.001). In conclusion, earlier subculture significantly shortens time to detection and time to actionable results. Some organisms may be missed by performing an early subculture, especially those that grow more slowly.

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Comparison of detection speed and yield in agitated and non‐agitated aerobic blood culture bottles

Cited by 11 publications

References 16 publications

The impact of the first notification of positive blood cultures on antibiotic therapy

The impact of the first notification of positive blood cultures on antibiotic therapy

Aetiology of neonatal sepsis in Nigeria, and relevance of Group b streptococcus: A systematic review

Nonautomated Blood Cultures in a Low-Resource Setting: Optimizing the Timing of Blind Subculture

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