Comparison of Diafiltration and Tangential Flow Filtration for Purification of Nanoparticle Suspensions

Dalwadi, Gautam; Benson, Heather A. E.; Chen, Yan

doi:10.1007/s11095-005-7781-z

Cited by 105 publications

(73 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Effective purification of nanoparticles is, therefore, a necessary step for controlling the quality and characteristics of nanoparticle Waghmare et al 139 products (Dalwadi et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis, optimization, purification and characterization of gold nanoparticles

Waghmare¹,

Deshmukh²,

Sadowski³

2014

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Many microorganisms produce intracellular metal nanoparticles. Aqueous gold, when exposed to several actinomycetal strains, become thereby, leading to the formation of gold nanoparticles. The use of microorganisms in the synthesis of nanoparticles is emerging as an eco-friendly and exciting approach gold for recovery. Streptomyces hygroscopicus was used for the biosynthesis of gold nanoparticles. UV and visible spectroscopic studies of biofilms revealed better synthesis of nanoparticles. It was observed that better biosynthesis of gold nanoparticles occurred when cell biomass treated with 10 -3 and 10 -4 mM HAuCl 4 solution as compared to other dilutions. The pH 7.0 was found to be optimum for the biosynthesis of gold nanoparticles. The TEM study revealS that there was evidence of gold nanoparticles synthesized by S. hygroscopicus. The results demonstrate that spherical gold nanoparticles in the range of 10 to 20 nm were observed at pH value of 7.0. The actinomycetal biomass and various concentration of aqueous HAuCl 4 solution were incubated, it was found that 10 -4 concentration shows excellent colour of the actinomycetal biomass.

show abstract

“…Effective purification of nanoparticles is, therefore, a necessary step for controlling the quality and characteristics of nanoparticle Waghmare et al 139 products (Dalwadi et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis, optimization, purification and characterization of gold nanoparticles

Waghmare¹,

Deshmukh²,

Sadowski³

2014

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…Tangential flow filtration (TFF), or cross-flow filtration, has been found as a very practical method to purify nanoparticulate dispersions from these impurities (5,13,(17)(18)(19)(20). It is a more gentle and continuous process and more easily scaled up than, e.g., the traditional dead-end ultrafiltration (21) or ultracentrifugation (22).…”

Section: Introductionmentioning

confidence: 99%

Effect of Sugars, Surfactant, and Tangential Flow Filtration on the Freeze-Drying of Poly(lactic acid) Nanoparticles

2009

View full text Add to dashboard Cite

Abstract. Poly(D,L-lactic acid) nanoparticles were freeze-dried in this study. With respect to drying, effect of protective excipients and purification from excess surfactant were evaluated. The nanoparticles were prepared by the nanoprecipitation method with or without a surfactant, poloxamer 188. The particles with the surfactant were used as such or purified by tangential flow filtration. The protective excipients tested were trehalose, sucrose, lactose, glucose, poloxamer 188, and some of their combinations. The best freeze-drying results in terms of nanoparticle survival were achieved with trehalose or sucrose at concentrations 5% and 2% and, on the other hand, with a combination of lactose and glucose. Purification of the nanoparticle dispersion from the excess surfactant prior to the freeze-drying by tangential flow filtration ensured better drying outcome and enabled reduction of the amount of the protective excipients used in the process. The excess surfactant, if not removed, was assumed to interact with the protective excipients decreasing their protective mechanism towards the nanoparticles.

show abstract

“…Finally, MNT offer the possibility of long-term storage, favorably distinguishing MNT from those nanoparticle systems that require preparation a relatively short time prior to use. 53,54 Finally, because of the modular nature of MNT design, one could envisage utilizing this platform to generate personalized therapeutics by tailoring the ligand and drug profiles to the characteristics of individual patients. Research grant (10-04-01037-a).…”

mentioning

confidence: 99%

Modular nanotransporters: a multipurpose in vivo working platform for targeted drug delivery

Slastnikova

Rosenkranz²,

Gulak³

et al. 2012

IJN

View full text Add to dashboard Cite

Background: Modular nanotransporters (MNT) are recombinant multifunctional polypeptides created to exploit a cascade of cellular processes, initiated with membrane receptor recognition to deliver selective short-range and highly cytotoxic therapeutics to the cell nucleus. This research was designed for in vivo concept testing for this drug delivery platform using two modular nanotransporters, one targeted to the α-melanocyte-stimulating hormone (αMSH) receptor overexpressed on melanoma cells and the other to the epidermal growth factor (EGF) receptor overexpressed on several cancers, including glioblastoma, and head-and-neck and breast carcinoma cells. Methods: In vivo targeting of the modular nanotransporter was determined by immunofluorescence confocal laser scanning microscopy and by accumulation of 125 I-labeled modular nanotransporters. The in vivo therapeutic effects of the modular nanotransporters were assessed by photodynamic therapy studies, given that the cytotoxicity of photosensitizers is critically dependent on their delivery to the cell nucleus.Results: Immunohistochemical analyses of tumor and neighboring normal tissues of mice injected with multifunctional nanotransporters demonstrated preferential uptake in tumor tissue, particularly in cell nuclei. With 125 I-labeled MNT{αMSH}, optimal tumor:muscle and tumor:skin ratios of 8:1 and 9.8:1, respectively, were observed 3 hours after injection in B16-F1 melanoma-bearing mice. Treatment with bacteriochlorin p-MNT{αMSH} yielded 89%-98% tumor growth inhibition and a two-fold increase in survival for mice with B16-F1 and Cloudman S91 melanomas. Likewise, treatment of A431 human epidermoid carcinoma-bearing mice with chlorin e 6 -MNT{EGF} resulted in 94% tumor growth inhibition compared with free chlorin e 6 , with 75% of animals surviving at 3 months compared with 0% and 20% for untreated and free chlorin e 6 -treated groups, respectively. Conclusion:The multifunctional nanotransporter approach provides a new in vivo functional platform for drug development that could, in principle, be applicable to any combination of cell surface receptor and agent (photosensitizers, oligonucleotides, radionuclides) requiring nuclear delivery to achieve maximum effectiveness.

show abstract

Comparison of Diafiltration and Tangential Flow Filtration for Purification of Nanoparticle Suspensions

Cited by 105 publications

References 24 publications

Biosynthesis, optimization, purification and characterization of gold nanoparticles

Biosynthesis, optimization, purification and characterization of gold nanoparticles

Effect of Sugars, Surfactant, and Tangential Flow Filtration on the Freeze-Drying of Poly(lactic acid) Nanoparticles

Modular nanotransporters: a multipurpose in vivo working platform for targeted drug delivery

Contact Info

Product

Resources

About