Comparison of diagnostic methods for detecting gastric Helicobacter-like organisms in dogs and cats

Happonen, Irmeli; Saari, Seppo; Castren, L.; Tyni, O.; Hänninen, Marja‐Liisa; Westermarck, Elias

doi:10.1016/s0021-9975(96)80034-x

Cited by 45 publications

(39 citation statements)

References 16 publications

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“…When the results of culture and biopsies taken from infected cats at 3.5, 8, and 12 months are combined, it is apparent that culture was positive for H. felis at 3 of 15 sampling points, PCR was positive for H. felis at 9 of 10 sampling points, modified Steiner staining was positive for HLOs at 15 of 15 sampling points, and urease tests were positive at 13 of 15 sampling points. These observations concur with studies with H. felis-infected mice and dogs with naturally acquired helicobacteriosis, in which microscopy was more sensitive than culture and urease (31,45). Our observation that PCR was sensitive and specific is in agreement with studies of mice and dogs infected with H. felis and humans and cats infected with H. pylori (18,35,56,63).…”

Section: Discussionsupporting

confidence: 85%

Helicobacter felisInfection Is Associated with Lymphoid Follicular Hyperplasia and Mild Gastritis but Normal Gastric Secretory Function in Cats

et al. 2000

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The relationship of Helicobacter felis, a bacterium observed in the stomachs of cats, to gastric disease is unclear. The objective of this study was to determine if H. felis infection alters gastric histopathology, proinflammatory cytokine expression, and secretory function and evokes a humoral immune response in cats. Five specific-pathogen-free (SPF) Helicobacter-free cats were studied before and for 1 year after oral inoculation with H. felis (ATCC 49179). Four SPF H. felis-uninfected cats served as controls. The stomachs of all five H. felis-inoculated cats became colonized, as determined by urease activity, histopathology, PCR, culture, and transmission electron microscopy of serial gastric biopsies at 0, 3, 5, 8, and 12 months. Uninoculated cats remained Helicobacter free. Lymphoid follicular hyperplasia, atrophy, and fibrosis were observed primarily in the pylorus of infected cats. Mild mononuclear inflammation was detected in both infected and uninfected cats, but was more extensive in infected cats, with pangastric inflammation, eosinophilic infiltrates, and cardia gastritis observed only in infected cats. No upregulation of antral mucosal interleukin 1␣ (IL-1␣), IL-1␤, or tumor necrosis factor alpha was detected by reverse transcription-PCR in any cat. The gastric secretory axes, assessed by fasting plasma gastrin, antral mucosal gastrin and somatostatin immunoreactivity, and pentagastrin-stimulated gastric acid secretion, were similar in both infected and uninfected cats. Gradual seroconversion (immunoglobulin G) was observed in four of five infected cats, with enzyme-linked immunosorbent assay values reaching 4؋ to 12؋ baseline 12 months postinfection. These findings indicate that H. felis infection in cats induces lymphoid follicular hyperplasia, mild gastritis, and seroconversion, but is associated with normal gastric secretory function.

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Section: Discussionsupporting

confidence: 85%

Helicobacter felisInfection Is Associated with Lymphoid Follicular Hyperplasia and Mild Gastritis but Normal Gastric Secretory Function in Cats

et al. 2000

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“…are frequently found in dogs [6][7][8]15 , not only in healthy animals 15 , but also in dogs with gastropathy simptoms 7 .…”

Section: Discussionmentioning

confidence: 99%

“…The gastric Helicobacter commonly found in dogs are Helicobacter bizzozeroni, Helicobacter felis, Helicobacter heilmannii and Helicobacter salomonis 8 .…”

Section: Introductionmentioning

confidence: 99%

Studies of distribution and recurrence of Helicobacter spp. gastric mucosa of dogs after triple therapy

et al. 2011

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Purpose:To analyze the triple antimicrobial therapy in positive Helicobacter spp. dogs and to investigate recurrence. Methods: A total of 20 dogs underwent endoscopy followed by gastric biopsy using the rapid urease test and histopathology stained with Giemsa. Ten animals were treated with triple therapy recommended for humans and divided into control and experimental group. The control group was kept in isolation while the experimental group was placed in contact with positive animals during 60 days. Results: The prevalence of infection in animals in this experiment was 100%, and more frequent in the fundus and the gastric body. Therapy for 7 days using clarithromycin, amoxicillin and lansoprazole was effective in 100% of the animals. Recurrence of the infection in 80% of dogs in the experimental group, while the control group remained eradicated after 60 days. Conclusion: Crowded environments associated with close contact with dogs infected with helicobacter are a determinant for transmission of Helicobacter spp. between canines. Key works: Helicobacter infections. Endoscopy. Gastric Mucosa. Dogs. RESUMOObjetivo: Avaliar a eficácia da terapia tríplice em cães naturalmente infectados pelo Helicobacter spp. e investigar a recorrência da infecção pelo contato com animais infectados. Métodos: Foram utilizados 20 cães, submetidos à endoscopia digestiva alta seguida de biopsia gástrica usando teste rápido da urease e histopatologia corada pelo Giemsa. Dez animais foram tratados com terapia tríplice preconizada para humanos e divididos em grupo controle e experimental. O grupo controle foi mantido em isolamento enquanto que o grupo experimento foi colocado em contato com os animais positivos durante 60 dias. Resultados: A prevalência da infecção nos animais deste experimento foi de 100%, e mais frequente no fundo e corpo gástrico. A terapia durante 7 dias empregando claritromicina, amoxicilina e lansoprazol foi eficaz em 100% dos animais. Houve recorrência da infecção em 80% dos cães do grupo experimental, enquanto que o grupo controle manteve-se erradicado após 60 dias. Conclusão: Ambientes aglomerados associado a íntimo contato com cães infectados por helicobactérias é fator determinante para transmissão do Helicobacter spp. entre caninos. Descritores: Infecção por Helicobacter . Endoscopia. Mucosa Gástrica. Cães. Acta Cirúrgica Brasileira -Vol. 26 (2) 2011 -83Studies of distribution and recurrence of Helicobacter spp. gastric mucosa of dogs after triple therapy

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“…In each histological sample, 10 microscopic visual fields were evaluated for helicobacteria amount at the superficial epithelium, in gastric pits, and deeper into the mucosa at glandulocytes (Happonen et al, 1996; Hermanns, Kregel, Breuer, & Lechner, 1995; Vorobjova, Hurlimann, Zimmermann, Uibo, & Halter, 2005). In each mucosa histological sample of the fundic gland region, the amount of helicobacteria was arranged into four groups (Happonen et al, 1998): no helicobacteria in one visual field -absent; 1-10 helicobacteria in one visual field -mild; 10-50 helicobacteria in one visual field -moderate; and more than 50 helicobacteria in one microscopic visual field -severe.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Number of Gastric Mucoid Epitheliocytes and Parietal Cells in Relation to the Amount of Helicobacteria in the Fundic Gland Region of Domestic Dogs (Canis Familiaris)

Bērziņa¹,

Birģele²

2014

Proceedings of the Latvia University of Agriculture

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Abstract. This study was done to evaluate the number of gastric mucoid epitheliocytes and parietal cells in relation to the amount of helicobacteria in the fundic gland region of 30 domestic dogs (Canis familiaris). Gastric mucosa samples were taken from the fundic gland region in eight strictly determined places to detect helicobacteria in 10 microscopic visual fields by histological examination, to find out the quantity of helicobacteria at the superficial epithelium, in gastric pits, and in fundic glands by classifying it as absent, mild, moderate, and severe, as well as to evaluate the number of mucoid epitheliocytes of the gastric superficial-pit epithelium and of the parietal cells of fundic glands. Out of all examined 2400 microscopic visual fields, mild and moderate amounts of helicobacteria localizing mainly at the superficial epithelium and in gastric pits were detected in 69.0-77.5% of cases, and only in around 20% of cases they were found in different amounts deeper into the fundic glands. The number of mucoid epitheliocytes (199±20 cells in one visual field) was only slightly larger in places with a severe amount of helicobacteria compared to places with no helicobacteria (195±16 mucoid epitheliocytes in one visual field). Also the number of parietal cells in the fundic glands did not differ markedly among the places with different amounts of helicobacteria; however, in places with a severe amount of helicobacteria, parietal cell count tended to decrease to 76±8 cells in one visual field, but in places free from helicobacteria there were 83±9 parietal cells in one visual field.

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Comparison of diagnostic methods for detecting gastric Helicobacter-like organisms in dogs and cats

Cited by 45 publications

References 16 publications

Helicobacter felisInfection Is Associated with Lymphoid Follicular Hyperplasia and Mild Gastritis but Normal Gastric Secretory Function in Cats

Helicobacter felisInfection Is Associated with Lymphoid Follicular Hyperplasia and Mild Gastritis but Normal Gastric Secretory Function in Cats

Studies of distribution and recurrence of Helicobacter spp. gastric mucosa of dogs after triple therapy

Evaluation of the Number of Gastric Mucoid Epitheliocytes and Parietal Cells in Relation to the Amount of Helicobacteria in the Fundic Gland Region of Domestic Dogs (Canis Familiaris)

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