Comparison of diagnostic tools with multiplex polymerase chain reaction for pediatric lower respiratory tract infection: A single center study

Chen, Yu-Shen; Liu, Po-Yen; Huang, Ya-Ling; Chen, Chiao-Shan; Chiu, Ling-Hui; Huang, Nuan-Ya; Hsieh, Kai‐Sheng; Chen, Yao-Shen

doi:10.1016/j.jmii.2012.07.016

Cited by 13 publications

(7 citation statements)

References 26 publications

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“…Use of multiplex PCR for pneumococcal capsular polysaccharide on urine might have slightly increased this yield. 26,27 Secular trends 21 may have led to a reduction in the prevalence of S. pneumoniae as a cause of pneumonia. Widespread use of pneumococcal polysaccharide vaccine in our population (about 85% of eligible patients served by MED-VAMC have been vaccinated) and conjugate vaccine in children in Houston (about 95% uptake in infants and toddlers) has certainly reduced the frequency of pneumococcal disease in adults by direct and indirect effects.…”

Section: Discussionmentioning

confidence: 99%

Can an etiologic agent be identified in adults who are hospitalized for community-acquired pneumonia: Results of a one-year study

Musher

Roig

Cazares

et al. 2013

Journal of Infection

196

133

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Only 23.2% of 259 patients admitted with a CAP syndrome had documented bacterial infection; another 26.6% had no identified bacterial etiology, but findings closely resembled those of bacterial infection. Nevertheless, all 259 received antibacterial therapy. Careful attention to the clinical picture may identify uninfected patients or those with viral infection, perhaps with reassurance by a non-elevated procalcitonin. Determining an etiologic diagnosis remains elusive. Better discriminators of bacterial infection are sorely needed.

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Section: Discussionmentioning

confidence: 99%

Can an etiologic agent be identified in adults who are hospitalized for community-acquired pneumonia: Results of a one-year study

Musher

Roig

Cazares

et al. 2013

Journal of Infection

196

133

View full text Add to dashboard Cite

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“…Polymerase chain reaction (PCR) technique has been shown to be reliable for diagnosing the pathogens, especially for those that are difficult to culture [ 4 ]. In addition, PCR method has high sensitivity and specificity in detecting multiple microorganisms and yields results faster than culture and serological methods [ 8 ]. Most importantly, the results of PCR are not affected by prior use of antibiotics.…”

Section: Introductionmentioning

confidence: 99%

The Clinical Significance of FilmArray Respiratory Panel in Diagnosing Community-Acquired Pneumonia

Chen

Hui-lan

Lin

et al. 2017

BioMed Research International

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Aim FilmArray Respiratory Panel (FilmArray RP) test is an emerging diagnostic method in fast detecting multiple respiratory pathogens; the methodology and clinical significance of FilmArray RP in community-acquired pneumonia (CAP) diagnosis were evaluated in this study. Methods Specimens from 74 patients with CAP were analyzed and compared using FilmArray RP, traditional multiple PCR assay, bacterial (or fungal) culture, and serological detection. Results FilmArray RP and multiplex PCR showed 100% coincidence rate in detecting coronaviruses 229E, OC43, HKU1, and NL63, human metapneumovirus, influenza A and B, and parainfluenza viruses (PIV1, PIV2, and PIV4). There were 15 viral specimens tested as disagreement positive results. FilmArray RP had higher detection rate in detecting dual viral and Mycoplasma pneumoniae infection. The positive bacteria (or fungi) were found in 25 specimens. Conclusions This study demonstrated the capability of FilmArray RP for simultaneous detection of broad-spectrum respiratory pathogens and potential use in facilitating better patient care.

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“…Scores were ranked based on Zeman DH principle and the total ranking scores were used to assess overall performance [18,19] [15][16][17][18][19][20]. Cost estimates were generated through consideration of all substantial resource inputs necessary to perform each diagnostic test, including laboratory overhead costs, personnel costs, laboratory equipment, consumable supplies, test reagents, sample collection, transportation, and sample decontamination.…”

Section: Test Performancementioning

confidence: 99%

A suggested algorithm for detection of multi drug-resistant tuberculosis in Zimbabwe

Makamure

Makumbirofa

Bandason

et al. 2017

J Infect Dev Ctries

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Introduction: Rapid genotypic and phenotypic methods for multi-drug-resistant-tuberculosis (MDR-TB) detection are now widely available. Zimbabwe adopted the use of GeneXpert-MTB/RIF, microscopic-observation-drug-susceptibility-assay (MODS) and Mycobacteria-GrowthIndicator-Tube (MGIT) drug-susceptibility-testing (DST). Data is limited on the ideal combination of use of these methods in resource limited settings. Methodology: Between August 2014 to July 2015, 211 sputa from MDR-TB suspects were tested with GeneXpert-MTB/RIF, MODS, manual-MGIT and Lowenstein-Jensen (LJ)-DST to determine diagnostic accuracy and turnaround-time (TAT), with LJ-DST as the gold standard. A performance score ranking table for diagnostic accuracy, TAT, costs, facilities and expertise requirements, was used to determine the most favourable tool. Results: GeneXpert-MTB/RIF sensitivity was 96% (95%CI:80-100) and specificity was 95% (95%CI:90-97). MODS sensitivity was 88% (95%CI:68-97) and specificity was 97% (95%CI:87-100). Manual MGIT-DST had slightly lower sensitivity of 80% (95%CI:59-93). Median time to detection of MDR-TB was <1 day (IQR:0-0) for Xpert, 14 days (IQR:11-31) for MODS, 21 days (IQR:7-22) for MGIT-DST and 28 days (IQR:25-28) for LJ-DST. Operational costs for MODS, MGIT-DST, and GeneXpert-MTB/RIF were $21.20, $27.52 and $39.76 respectively. From a summation of scores including facility and expertise requirements per diagnostic technique, GeneXpert-MTB/RIF was the most favourable tool, followed by MODS and MGIT-DST. Conclusions: For best scale-up of MDR-TB diagnosis in Zimbabwe, GeneXpert-MTB/RIF can be used for rapid detection of TB in smear negative cases, RIF-susceptibility for early treatment initiation and probable MDR-TB. MODS can rapidly confirm probable MDR-TB detected by GeneXpert-MTB/RIF, manual-MGIT can provide early results for susceptibility to other antibiotics, with affordable costs, with LJ-DST confirming discordant DSTs.

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Comparison of diagnostic tools with multiplex polymerase chain reaction for pediatric lower respiratory tract infection: A single center study

Cited by 13 publications

References 26 publications

Can an etiologic agent be identified in adults who are hospitalized for community-acquired pneumonia: Results of a one-year study

Can an etiologic agent be identified in adults who are hospitalized for community-acquired pneumonia: Results of a one-year study

The Clinical Significance of FilmArray Respiratory Panel in Diagnosing Community-Acquired Pneumonia

A suggested algorithm for detection of multi drug-resistant tuberculosis in Zimbabwe

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