Comparison of Dietary Casein and Soybean Protein Effects on Plasma Lipid and Gastrin Levels, Hepatic &amp;Delta;&lt;sup&gt;6-&lt;/sup&gt;Desaturase Activity and Coronary Arteriosclerosis in Male Sprague-Dawley Rats

Lindholm, Marie; Sjöblom, Linnéa; Nordborg, Claes; Östlund-Lindqvist, Ann-Margret; Eklund, Anders

doi:10.1159/000177781

Cited by 9 publications

(4 citation statements)

References 17 publications

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“…Lindhom et al (1993) found the same results in rats who were fed a diet containing 20 per cent casein or soy protein supplemented with 0.5 per cent of cholesterol for 28 days. Moreover, in rats who were fed with a diet containing 20 per cent casein or soy protein and supplemented with 10 per cent of the olive oil and supplemented with cholesterol for 28 days, Eklund and Sjoblom (1986) noted that when dietary cholesterol increases from 0 to 0.25 per cent, the content of serum TG increases with casein or soy protein combined.…”

supporting

confidence: 52%

Olive or salmon oils affect differently the storage and transport of fatty acids by VLDL in hypercholesterolemic rats fed different proteins

Bouderbala

Bouchenak

2016

Nutrition &Amp; Food Science

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Purpose – The purpose of this study is to compare the effect of olive or salmon oil on the hepatic storage and transport of fatty acids by very-low-density lipoproteins (VLDL). Design/methodology/approach – In all, 24 male Wistar rats (80 ± 5 g) were fed a 0.5 per cent cholesterol-enriched diet with either 20 per cent casein (C) or chickpea (CP) proteins with 10 per cent olive (O) or salmon (S) oil for 28 days. Findings – In VLDL-triacyglycerols fatty acids, oleic acid content was higher in CPS as compared to that in CS or CPO and lower in CS and CPO than that in CO; linoleic acid content was higher in all groups; arachidonic acid content was higher in CS and CPO as compared to that in CO. In the liver, TG fatty acids content was lower in CPO or CPS as compared to that in CO or CS; oleic and arachidonic acid contents were lower in CPS than that in CPO; linoleic acid content was lower in CS, CPS and CPO than that in CO, CPO and CO. In liver, phospholipid fatty acid, oleic and arachidonic acid contents were lower in CPS than that in CS; oleic, linoleic and arachidonic acid contents were lower in CPO compared to that in CO. In liver, cholesteryl esters fatty acids, oleic, linoleic and arachidonic acids contents were higher in CPS as compared to that in CS; oleic, linoleic and arachidonic acid contents were lower in CS as compared to that in CO; linoleic and arachidonic acid contents were lower in CPS than that in CPO. Originality/value – A cholesterol-enriched diet containing casein or chickpea proteins combined with olive or salmon oil affects the hepatic storage and transport of polyunsaturated and monounsaturated fatty acids by VLDL.

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supporting

confidence: 52%

Olive or salmon oils affect differently the storage and transport of fatty acids by VLDL in hypercholesterolemic rats fed different proteins

Bouderbala

Bouchenak

2016

Nutrition &Amp; Food Science

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“…For experiments, both monocytes and macrophages were incubated for 24 h in serum-free media with lipoprotein additions as indicated under "Results." Lipoproteins were prepared by differential ultracentrifugation of fresh plasma from healthy donors (29).…”

Section: Methodsmentioning

confidence: 99%

DNA Methylation Polymorphisms Precede Any Histological Sign of Atherosclerosis in Mice Lacking Apolipoprotein E

Lund¹,

Andersson

Lauria³

et al. 2004

Journal of Biological Chemistry

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295

202

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The present work investigates the occurrence and significance of aberrant DNA methylation patterns during early stages of atherosclerosis. To this end, we asked whether the genetically atherosclerosis-prone APOEnull mice show any changes in DNA methylation patterns before the appearance of histologically detectable vascular lesion. We exploited a combination of various techniques: DNA fingerprinting, in vitro methyl-accepting assay, 5-methylcytosine quantitation, histone posttranslational modification analysis, Southern blotting, and PCR. Our results show that alterations in DNA methylation profiles, including both hyper-and hypomethylation, were present in aortas and PBMC of 4-week-old mutant mice with no detectable atherosclerotic lesion. Sequencing and expression analysis of 60 leukocytic polymorphisms revealed that epigenetic changes involve transcribed genic sequences, as well as repeated interspersed elements. Furthermore, we showed for the first time that atherogenic lipoproteins promote global DNA hypermethylation in a human monocyte cell line. Taken together, our results unequivocally show that alterations in DNA methylation profiles are early markers of atherosclerosis in a mouse model and may play a causative role in atherogenesis.Atherosclerosis and its complications are a major cause of death and disability in the developed world. The disease is characterized by infiltration of lipid particles in the arterial wall, accompanied by the recruitment of inflammatory and immune cells, migration and proliferation of smooth muscle cells (SMC), 1 and synthesis of extracellular matrix. These processes eventually result in the gradual development of an elevated lipid-rich, fibrocellular lesion (1).In mammals, DNA methyltransferases use S-adenosyl methionine (SAM) as a methyl group donor to methylate the carbon in position 5 of cytosine residues in a CpG dinucleotide (CG) context (2). DNA methylation regulates fundamental biological phenomena such as gene expression, genome stability, mutation rate, genomic imprinting, and X chromosome inactivation (3-6). Both global and gene-specific alterations in DNA methylation are associated with abnormal phenotypes in disease (7,8). For example, cancer cells show global genomic hypomethylation and dense hypermethylation of CpG islands, which are normally unmethylated (9). The identification of cancer type-and stage-specific changes in DNA methylation has justified hopes for novel diagnostic and therapeutic avenues (10).Two general observations suggest that alterations in DNA methylation patterns are involved in atherogenesis (11-13). First, global hypomethylation and dense hypermethylation of certain CpG islands are associated with aging, a major risk factor for atherosclerosis (14). Second, hyperhomocysteinemia and the subsequent decreased production or bioavailability of SAM is associated with an increased risk of cardiovascular disease (15). Accordingly, mice with genetically reduced levels of methylenetetrahydrofolate reductase, a key enzyme in the pathway generating ...

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“…Lipoproteins were prepared by differential ultra centrifugation of fresh plasma from healthy donors [ 16 ]. Lipoproteins were stored at -80°C with 10% sucrose and 4.8 mM EDTA, pH = 7.4, a procedure which does not affect lipoprotein integrity [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Cytokine response to lipoprotein lipid loading in human monocyte-derived macrophages

2006

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Background: Macrophage foam cell formation is a prominent feature of human atherosclerotic plaques, usually considered to be correlated to uptake of and inflammatory response to oxidized low density lipoproteins (OxLDL). However, there are alternative pathways for formation of macrophage foam cells and the effect of such lipid loading on macrophage function remains to be fully characterized. In the present study we investigated basal and inducible cytokine expression in primary human macrophages either loaded with triglycerides through incubation with very low density lipoproteins (VLDL) or with cholesterol through incubation with aggregated LDL (AgLDL). We then analyzed how foam cell lipid content affected secretion of three pro-inflammatory cytokines: interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), and of one chemokine: interleukin-8 (IL-8), all of which are considered pro-inflammatory, proatherosclerotic, and are expressed by cells in atherosclerotic tissue.

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Comparison of Dietary Casein and Soybean Protein Effects on Plasma Lipid and Gastrin Levels, Hepatic Δ<sup>6-</sup>Desaturase Activity and Coronary Arteriosclerosis in Male Sprague-Dawley Rats

Cited by 9 publications

References 17 publications

Olive or salmon oils affect differently the storage and transport of fatty acids by VLDL in hypercholesterolemic rats fed different proteins

Olive or salmon oils affect differently the storage and transport of fatty acids by VLDL in hypercholesterolemic rats fed different proteins

DNA Methylation Polymorphisms Precede Any Histological Sign of Atherosclerosis in Mice Lacking Apolipoprotein E

Cytokine response to lipoprotein lipid loading in human monocyte-derived macrophages

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