Comparison of Different Bone Filling Materials and Resorbable Membranes by Means of Micro-Tomography. A Preliminary Study in Rabbits

Abstract

The purpose of this work was to evaluate the behavior of different membranes and bone filling materials used to fill critical defects in rabbit calvaria. Four defects were prepared in the cranial calvaria of female rabbits. They were randomly divided into three subgroups according to the type of barrier membrane to be used. Four animals carried cross-linked bovine collagen membranes (Mem-Lok, Bio-Horizons, Birmingham, AL, USA)), four human fascia lata membranes (Tissue, Inbiomed SA, Córdoba, Argentina) and four human chorioamniotic membranes (Tissue. Inbiomed SA, Córdoba, Argentina). The defects were filled with the deproteinized bovine bone particulate Bio­Oss® (Geistlich­Pharma AG, Wolhusen, Switzerland), with particulate human hydroxyapatite MinerOss® (Bio-Horizons, Birmingham, AL, USA), with particulate dental material (Tissue Bank Foundation, Inbiomed S.A., Córdoba, Argentina), and the last one was left without the addition of filler material. In the first group of four specimens, a resorbable cross-linked bovine collagen membrane was placed over the skull and defects, without additional fixing. In the second group, a human fascia lata membrane was placed, without additional fixing. In the third group, a human chorioamniotic membrane was placed, without additional fixing. The animals were sacrificed at 4 and 8 weeks. The highest percentages of relative radiological density (average) were recorded considering the amnio-chorionic membranes (83.63%) followed by collagen (81.44%) and finally the fascia lata membranes (80.63%), but the differences were not statistically significant (p > 0.05). The sites grafted with a decellularized tooth (96.83%) and Bio­Oss (88.42%), recorded the highest percentages of radiological density but did not differ significantly from each other (subset 2). The three membranes used did not show statistical differences between them, in any of the two time periods used. There were statistical differences between the filling materials evidencing the presence of a large quantity of calcified material in the defects treated with particulate tooth and deproteinized bovine bone and while smaller amounts of calcified material were registered in the case of defects treated with human hydroxyapatite and those that were not treated.

Photobiomodulation Therapy (PBMT) Applied in Bone Reconstructive Surgery Using Bovine Bone Grafts: A Systematic Review

“…Efforts to minimize complications and the time needed to heal by improving the process and enhancing biocompatibility has led to the emergence of PBMT-associated biomaterial application in the world literature [12,31]. Bovine bone is listed as the most frequently used type of graft in the literature for the reconstructive bone process [15,20,[36][37][38][39].…”

“…The literature cites as necessary in the reconstruction of bone defects three simultaneous conditions: (i) osteoconductive properties; (ii) inductive properties; and (iii) the presence of bone-forming cells [35]. As an alternative material, bovine bone graft [15,20,[36][37][38][39] is the most widely used due to its biocompatibility characteristics, as indicated in reconstructive areas related to traumatology, cranio-maxillary surgery, facial prosthetic rehabilitation, skeletal aging and esthetic aging [28]. Physical methods, such as low-intensity ultrasound (LIPUS) [40] and photobiomodulation therapy (PBMT), have the potential to improve the bone reconstruction process, acting or not in combination with bone grafts.…”

Morphology Analysis of Human Fourth Molars Using Computed Microtomography - Pilot Study

“…Hyperdontia is the term used to describe extra or supernumerary teeth that exceed the 20 deciduous teeth or 32 permanent teeth 23 . Atavism, by definition, is the reappearance in an individual of characteristics of some remote ancestor 24 and it supports some theories for fourth molars development. Hyperdontia may occur due to a division of tooth germ and also as a consequence of genetic and circumstantial factors [25][26][27][28] .…”

In Vivo Study of Nasal Bone Reconstruction with Collagen, Elastin and Chitosan Membranes in Abstainer and Alcoholic Rats

“…Collagen-containing natural membranes are the most common material used for GBR, with their presence or degradation not exerting any deleterious effect on tissues. However, their main limitation is the lack of stiffness [ 11 , 15 , 16 ]. In addition to low immunogenicity, collagen membranes provide support for angiogenesis, an important feature since the developing bone tissue depends on blood vessels to supply it with nutrients and oxygen [ 17 , 18 ].…”