Comparison of Different Carriers to Maintain a Stable Partial Nitrification Process for Low-Strength Wastewater Treatment

Abstract

Practical application of the partial nitritation–anaerobic ammonium oxidation (anammox) process has attracted increasing attention because of its low operational costs. However, the nitritation process, as a promising way to supply nitrite for anammox, is sensitive to the variations in substrate concentration and dissolved oxygen (DO) concentration. Therefore, a stable supply of nitrite becomes a real bottleneck in partial nitritation–anammox process, limiting their potential for application in mainstream wastewater treatment. In this study, five 18-L sequencing batch reactors were operated in parallel at room temperature (22°C ± 4°C) to explore the nitritation performance with different carrier materials, including sepiolite-nonwoven carrier (R1), zeolite-nonwoven carrier (R2), brucite-nonwoven carrier (R3), polyurethane carrier (R4), and nonwoven carrier (R5). The ammonia oxidation rate (AOR) in R1 reached the highest level of 0.174 g-N L−1 d−1 in phase II, which was 1.4-fold higher than the control reactor (R4). To guarantee a stable supply of nitrite for anammox process, the nitrite accumulation efficiency (NAE) was always higher than 77%, even though the free ammonia (FA) decreases to 0.08 mg-N/L, and the pH decreases to 6.8 ± 0.3. In phase V, the AOR in R1 reached 0.206 g-N L−1 d−1 after the DO content increase from 0.7 ± 0.3 mg/L to 1.7 ± 0.3 mg/L. The NAE in R1 was consistently higher than 68.6%, which was much higher than the other reactor systems (R2: 43.8%, R3: 46.6%, R4: 23.7%, R5: 22.7%). Analysis of 16S rRNA gene sequencing revealed that the relative abundance of Nitrobacter and Nitrospira in R1 was significantly lower than other reactors, indicating that the sepiolite carrier plays an important role in the inhibition of nitrite-oxidizing bacteria. These results indicate that the sepiolite nonwoven composite carrier can effectively improve the nitritation process, which is highly beneficial for the application of partial nitritation–anammox for mainstream wastewater treatment.

Concept development of a mainstream deammonification and comparison with conventional process in terms of energy, performance and economical construction perspectives

“…Since the last decades, there have been many studies conducted on the determination of optimal conditions for deammonification, the effect of reactor technologies such as moving bed biofilm reactor (MBBR) and configurations such as integrated fixed-film activated sludge (IFAS), the impact of different biofilm carriers and additives such as salts and polymers for the process stability, the identification of the diversity and distribution of involved microorganisms and examination of their growth conditions ( Liu et al, 2021 ; Feng et al, 2022 ; Jiang et al, 2022 ; Ladipo-Obasa et al, 2022 ; Schoepflin et al, 2022 ; Zhang et al, 2022 ). So far, most of the studies have been carried out on a laboratory scale with artificial wastewater.…”