Comparison of Different Commercial Serology Kits for the Determination of Seropositive Toxoplasmosis in Cattle in Indonesia

Abstract

This research aims to explain the evaluation of the differences in four commercial kits for the detection of serological toxoplasmosis used in Indonesia. The results of the study found that the toxoplasmosis seropositivity determined by the four commercial kits showed a significant difference (P0.05). Seropositive toxoplasmosis obtained using Pastorex, Toxotest, IDScreen, and Toxo Ab were 35.12%, 60.12%, 26.19%, and 10.12% respectively. IDScreen had a good agreement with Toxo Ab (Gwet's AC1= 0.623) and a moderate agreement with Pastorex (Gwet's AC1= 0.494-0.511). Toxotest had a low agreement with three commercial kits (Gwet's AC1= 0.2) but had a moderate agreement with western blotting (WB) and modified agglutination test (MAT) (Gwet's AC1= 0.458-0.557).

Evaluation of In‐House ELISA for Antirabies Antibodies Detection in Domestic Canine

“…Interassay agreement among the three rabies ELISA kits was carried out on all of 158 known positive and negative serums or also partially on known positive serum from dogs vaccinated with Rabivet® or Rabisin®. The analysis of the results of interassay agreement among the three tests of the rabies ELISA kit was carried out using Agreestat360 [ 20 ] and the interpretation of the level of agreement was based on the benchmark from Altman [ 21 ]. Serial and parallel test simulations among the three ELISA kits were evaluated to determine the improvement in diagnostic test performance that could be achieved with the combination of the two ELISA kits.…”

“…A total of 48 known positive and negative serums were selected randomly, and the results of their agreement were compared between the three ELISA methods with RFFIT. The test results were analyzed by Agreestat360 [ 20 ], and the interpretation of the level of concordance was based on the benchmark from Altman [ 21 ].…”