Comparison of different cytogenetic methods and tissue suitability for the study of chromosomes in Cimex lectularius (Heteroptera, Cimicidae)

Sadílek, David; Angus, Robert B.; Šťáhlavský, František; Vilímová, Jitka

doi:10.3897/ccompcytogen.v10i4.10681

Cited by 3 publications

(3 citation statements)

References 67 publications

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“…C. lectularius gonads were used for the cytogenetic analysis of chromosomes by the spreading technique (25,29). The diploid chromosome number was determined from 10 chromosome photographs of mitotic metaphases and meiotic metaphases I and II per specimen.…”

Section: Methodsmentioning

confidence: 99%

“…The diploid chromosome number was determined from 10 chromosome photographs of mitotic metaphases and meiotic metaphases I and II per specimen. However, sex chromosomes can be clearly identified solely in metaphase II, observable in males only, as a result of inverted meiosis of sex chromosomes (25,29). The whole intestine was removed because its presence could influence the FCM analysis.…”

Section: Methodsmentioning

confidence: 99%

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Nuclear Genome Size in Contrast to Sex Chromosome Number Variability in the Human Bed Bug, Cimex lectularius (Heteroptera: Cimicidae)

et al. 2019

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The human bed bug Cimex lectularius is one of the most prevalent human ectoparasites in temperate climate zones. The cytogenetic features of this resilient pest include holokinetic chromosomes, special chromosome behavior in meiosis, and numerical variation of chromosomes, where the diploid number ranges from 26 + X1X2Y to 26 + X1–20Y. It is desirable to assess the nuclear DNA content of various cytotypes for a further detailed study of the C. lectularius genome. Detailed knowledge of the DNA content of this parasite could also clarify the origin of additional chromosomes. The average nuclear genome size C. lectularius with 2n = 26 + X1X2Y is 2C = 1.94 pg for males and 1.95 pg for females. There is a significant correlation between genome size and the number of chromosomes, but in some specimens with additional chromosomes, nuclear genome size decreases or remains average. Several species used as the internal reference standard were tested for further investigations of genome size in C. lectularius, and the plant Solanum pseudocaspicum turned out to be the most suitable. © 2019 International Society for Advancement of Cytometry

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Nuclear Genome Size in Contrast to Sex Chromosome Number Variability in the Human Bed Bug, Cimex lectularius (Heteroptera: Cimicidae)

et al. 2019

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“…Cytologists have been greatly hampered in the study of insect chromosomes by the fact that some species possess small size and/or a high number of chromosomes which usually makes counting and observing individual chromosomes difficult. Therefore, remarkable research interest has been focused on the development of reliable and efficient cytogenetic techniques to generate good chromosome preparations, applicable to a wide variety of species and insect tissues (Sadílek et al 2016). Observations of chromosomes, including banding patterns are usually made by locating cells in metaphase, the stage of cell division where chromosomes are greatly condensed and positions in the Equator of the dividing cells.…”

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confidence: 99%

Some Cytogenetic Methods for the Investigation of Insect Chromosomes and Their Implications for Research in Systematic Entomology

Koçak¹,

Okutaner²

2017

LEB

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Abstract:The use of karyological studies in insects is of considerable significance. However, chromosome karyotyping has been hampered principally by: 1) the minute size of many insects, 2) the recovery of suitable tissues, 3) the small number of cells adequate for analyses 4) the smallness of the chromosomes in some groups, 5) the difficulty in obtaining satisfactory numbers of well-spread or dispersed metaphases, and 6) the determination of the best time of the year to carry out chromosome preparations using freshly collected material because the life history of many insects is still not well-known. Many attempts have been made to reduce these difficulties by developing novel karyological methods. These procedures can yield chromosome preparations applicable to a wide variety of insects. In this paper, we outline techniques for preparation and observation of chromosomes from insect cells. Also, implications of chromosome techniques on insect systematics are discussed.

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Genome Size and Sex Chromosome Variability of Bed Bugs Feeding on Animal Hosts Compared to Cimex lectularius Parasitizing Human (Heteroptera: Cimicidae)

2019

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Genome size and chromosome number of five Cimicidae species were compared with the similar data recently received from Cimex lectularius parasitizing human. The average nuclear DNA content (males) was 2C = 1.47 pg in C. hemipterus, 2C = 1.61 pg in C. hirundinis, 2C = 1.80 pg in C. lectularius from bats, 2C = 1.68 pg in C. pipistrelli, and 2C = 1.22 pg in Paracimex cf. chaeturus. In the genomes of all cimicid species analyzed, the average GC content ranged from 32.74% in C. pipistrelli to 35.87% in P. cf. chaeturus. Chromosome variability with two male cytotypes, 2n = 28 + X 1 X 2 Y and 28 + X 1 X 2 X 3 Y, was confirmed in C. pipistrelli. In addition, intraspecific variability in chromosome number was revealed in C. lectularius from bats with 2n = 26 + X 1 X 2 Y and 26 + X 1 X 2 X 3 Y. We suggest that the origin of intraspecific variability in chromosome number of C. lectularius from bats and C. pipistrelli is not only the result of simple fragmentation, but additive rearrangements like duplications are probably also involved.

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Comparison of different cytogenetic methods and tissue suitability for the study of chromosomes in Cimex lectularius (Heteroptera, Cimicidae)

Cited by 3 publications

References 67 publications

Nuclear Genome Size in Contrast to Sex Chromosome Number Variability in the Human Bed Bug, Cimex lectularius (Heteroptera: Cimicidae)

Nuclear Genome Size in Contrast to Sex Chromosome Number Variability in the Human Bed Bug, Cimex lectularius (Heteroptera: Cimicidae)

Some Cytogenetic Methods for the Investigation of Insect Chromosomes and Their Implications for Research in Systematic Entomology

Genome Size and Sex Chromosome Variability of Bed Bugs Feeding on Animal Hosts Compared to Cimex lectularius Parasitizing Human (Heteroptera: Cimicidae)

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