Comparison of different diagnostic test to detect feline panleukopenia virus among cats in Kerala, India

Raheena, Koulath P.; Priya, P. M.; Mani, Binu K.; Mini, M.; Pillai, Usha Narayana

doi:10.18805/ijar.10975

Cited by 5 publications

(5 citation statements)

References 7 publications

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“…Early and timely diagnosis is imperative for effective disease management and containment, vital in halting the dissemination of FPLV. Raheena et al (2017) found that, PCR is a sensitive, specific and rapid technique for FPV detection, while sensitivity of LFA is poor.…”

Section: Treatment and Discussionmentioning

confidence: 98%

Lateral Flow Assay and Polymerase Chain Reaction Based Diagnosis and Management of Feline Parvoviral Infection in a Persian Kitten

P V

2024

JIVA

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Felineparvoviral infection, caused by the highly contagious feline panleukopenia virus (FPV), poses a significant threat to feline populations worldwide. This paper presents a case study detailing the successful diagnosis and management of feline parvoviral infection in a seven-month-old Persian kitten.Diagnostic evaluation was performed using a comprehensive approach including Complete Blood Count (CBC), Lateral flow assay and Polymerase Chain Reaction (PCR).The treatment focused on administering supportive care along with antibiotics. In conclusion, this case study underscores the importance of using appropriate molecular diagnostic techniques like LFA and PCR for timely prompt diagnosis and aggressive supportive care in the management of feline parvoviral infection, infection, particularly in susceptible breeds like Persian cats.

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Section: Treatment and Discussionmentioning

confidence: 98%

Lateral Flow Assay and Polymerase Chain Reaction Based Diagnosis and Management of Feline Parvoviral Infection in a Persian Kitten

P V

2024

JIVA

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“…The virus isolation can be used for diagnostic purposes. However, it requires enormous effort, time, and specialized personnel, and additional tests such as immunofluorescence and hemagglutination must be performed to detect the viral antigens (Raheena et al 2017). Due to its high sensitivity and specificity, the PCR technique is widely applied to diagnose several diseases (Chowdhury et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of carnivore protoparvovirus strains circulating in cats in Turkey

et al. 2023

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Cats are susceptible to feline panleukopenia virus (FPV) and canine parvovirus type 2 (CPV-2). Therefore, coinfection and superinfection with multiple parvovirus strains may occur, resulting in high heterogeneity and recombination. Considering the importance of cats as a potential source of genetic diversity for parvoviruses, we investigated the frequency of parvovirus infection in cats using their blood and fecal samples and performed molecular characterization of parvovirus strains circulating in cat populations. Accordingly, the fecal and blood samples of 60 cats with gastroenteritis symptoms were collected from Turkey’s Burdur, Isparta, and Izmit provinces. Of these 15 fecal samples tested as parvovirus-positive by PCR, 14 were confirmed to have been infected with true FPV strains by sequencing analysis. Through the phylogeny analysis, those were located in the FPV cluster, closely related to CPV-2, and one was discriminated in the CPV-2b cluster. Additionally, sequence analysis of the VP2 gene of CPV and FPV revealed that the FPV strains detected in Turkey and the vaccine strains were highly related to each other, with a nucleotide identity of 97.7- 100%. Furthermore, 13 variable positions were detected in VP2 of the field and reference FPV strains. Three synonymous mutations were determined in the VP2 gene. Some amino acid mutations in the VP2 protein-affected sites were considered responsible for the virus’s biological and antigenic properties. The partial sequence analysis of the VP2 gene revealed that four FPV strains detected in Turkey have a single nucleotide change from T to G at the amino acid position 384 between the nucleotides 3939-3941, which was reported for the first time. Therefore, these four isolates formed a different branch in the phylogenetic tree. The results suggest that both FPV and CPV-2b strains are circulating in domestic cats in Turkey and cats should be considered as potential sources of new parvovirus variants for cats, dogs and other animals.

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“…These finding disagreements with Awad et al [16] they mentioned that the sensitivity, specificity and accuracy of ELISA were 88%, 100%, and 94.5% respectively. Raheena et al [48] stated that polymerase chain reaction (PCR) assay is considered as a highly sensitive, specific and rapid technique for confirmative diagnosis of feline panleukopenia virus (FPV) infection in cats.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Feline Panleukopenia Virus in Cats in Duhok Province, Iraq

Dishow,

Alsarhan,

Al-Naqshabendy

2023

Egyptian Journal of Veterinary Sciences

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T HE current study targeted evaluation the prevalence of feline panleukopenia virus (FPV) in cats in Duhok province-Iraq, using immunochromatography assay (ICA) as a rapid test, indirect enzyme immunosorbent assay (i-ELISA) and conventional polymerase chain reaction (c-PCR). Moreover, determining the compatibility, sensitivity, specificity and accuracy between the different diagnostic techniques. A total of 100 fecal swab samples were collected from 52 household cats and 48 stray cats in various regions in Duhok province and tested using Rapid test and c-PCR, also 100 blood samples were drawn from cephalic and/or saphenous veins of same cats and tested using i-ELISA. The overall prevalence of FPV was 40%, 66% and 70% using rapid test, i-ELISA and c-PCR technique respectively. The prevalence was significantly higher in stray cats compared with household cats according to all tests used in this study. A moderate compatibility observed between rapid test and c-PCR based on Kappa value (0.440) with sensitivity 57.14%, specificity 100% and accuracy 70% of rapid test compared with c-PCR. Moreover, Fair compatibility between i-ELISA and c-PCR technique based on Kappa value (0.312) with sensitivity 75.71% , specificity 56.66%, and accuracy 70% of i-ELISA compared with c-PCR technique. It has been conclude that FPV is widespread in cats at Duhok province-Iraq. However, stray cats have a significant role in spreading of disease, and the rapid test and i-ELISA need to confirm using c-PCR technique.

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Comparison of different diagnostic test to detect feline panleukopenia virus among cats in Kerala, India

Cited by 5 publications

References 7 publications

Lateral Flow Assay and Polymerase Chain Reaction Based Diagnosis and Management of Feline Parvoviral Infection in a Persian Kitten

Lateral Flow Assay and Polymerase Chain Reaction Based Diagnosis and Management of Feline Parvoviral Infection in a Persian Kitten

Molecular characterization of carnivore protoparvovirus strains circulating in cats in Turkey

Prevalence of Feline Panleukopenia Virus in Cats in Duhok Province, Iraq

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