Comparison of different extenders and storage temperature on the sperm motility characteristics of Kolbroek pig semen

Mapeka, M. H.; Lehloenya, K.C.; Nedambale, T. L.

doi:10.4314/sajas.v42i5.18

Cited by 8 publications

(9 citation statements)

References 12 publications

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“…In the liquid semen storage, the study showed that there was no significant reduction in percentage of sperm motility and 9.35 ± 0.3 aa 9.59 ± 0.6 ab 9.14 ± 0.5 ab 8.98 ± 0.8 bc 7.36 ± 0.18 * Data shown as Mean ± SE, values with different superscript in the column differ significantly (P<0.05), *values differ significantly (P<0.01) in the respective column viability from day 0 to day 3 although there was linear decline in percentage of sperm motility, viability and plasma membrane integrity with the advancement of storage days (Table 1). Similar report was observed in the earlier studies (Mapeka et al, 2012. The sperm motility reduces in the liquid semen storage over the period due to the fact that there was a decrease in membrane integrity and thereby initiating the acrosome reaction during storage.…”

Section: Resultssupporting

confidence: 89%

Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India

Kadirvel

Kalita

Dewry

et al. 2018

IJAR

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Study was conducted to compare the semen quality and fertility of liquid stored semen for three days and frozen-thawed semen in the north-eastern region of India. For liquid semen, the semen ejaculates were extended in Beltsville Thawing Solution (BTS) extender and preserved at 17°C for three days. For cryopreservation, semen was diluted Lactose-egg yolk-glycerol extender and frozen in straw using programmable freezer with freezing rate of 40°C/min from -6 to -140°C. The preserved evaluated for sperm motility, viability, plasma membrane integrity and fertility. The results revealed that the liquid stored semen has maintained the sperm motility and viability up to day 3 without significant reduction. Similarly the plasma membrane integrity did not differ significantly up to day 2, but it was significantly (P less than 0.05) reduced on days 3 in liquid stored semen. After freezing and thawing, the mean sperm motility, viability and plasma membrane integrity were 58.25 ± 2.96%, 64.75 ± 2.47% and 47.06 ± 2.02%, respectively. These parameters were significantly (PP less than 0.01) lower as compared to the liquid stored semen from day 0 to day 3. After insemination with liquid semen, the farrowing rate was 77.7%, 80.76%, 73.07% and 69.8%, respectively from day 0, day1, day 2 and day 3. The pregnancy rate, farrowing rate and litter size did not differ significantly among different days of liquid storage. These parameters were significantly (PP less than 0.01) lower in frozen semen as compare to that of liquid stored semen. The study concluded that the liquid semen stored up to three days is more efficient than frozen-thawed semen in terms of preserving sperm quality and fertility.

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Section: Resultssupporting

confidence: 89%

Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India

Kadirvel

Kalita

Dewry

et al. 2018

IJAR

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“…Consequently, boar semen fertility remains an area of interest in the pig farming and industries alike [2] [3]. Large White and Kolbroek boars are popular with our local pig farmers due to their desirable traits including their superior semen motility parameters accompanied by high sperm cell viabilities [4] [5]. The current semen evaluation techniques, such as CASA, provide a very conservative estimate of their relative fertility [4] [5].…”

Section: Introductionmentioning

confidence: 99%

“…Large White and Kolbroek boars are popular with our local pig farmers due to their desirable traits including their superior semen motility parameters accompanied by high sperm cell viabilities [4] [5]. The current semen evaluation techniques, such as CASA, provide a very conservative estimate of their relative fertility [4] [5]. These observations have led to interests in the investigations of fertility markers in boars [3] [6] [7] [8].…”

Section: Introductionmentioning

confidence: 99%

An Investigative Immunoassay Targeting Two Osteopontin Epitopes in Boar Semen

Pilane¹,

Netshirovha²,

Tshabalala³

2020

OJAS

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The identification of superior sire is largely dependent on the semen quality evaluations, currently through computer-aided sperm class analyser (CASA). Immunoassays present a viable method for analysis of proteins associated with fertility, such as osteopontin. We have targeted osteopontin at an upstream epitope at position 249-267 amino acids and a downstream epitope at position 59-74 amino acids of full osteopontin protein in raw, BTS, Kobidil, Tris and Citrate liquid preserved Large white and Kolbroek boar semen. The Genetool analysis software was used to quantify the amount of osteopontin detected. Three boars per breed were used and experiments were repeated five times. Our results revealed that the downstream 15 amino acid epitope was more sensitive to osteopontin antibody. Between total motility and osteopontin, Kolbroek boar semen preserved in Kobidil, Citrate, BTS and Tris extenders revealed positive correlations of 0.564, 0.471, −0.960 and 0.330, respectively. Large white boars semen showed correlations of −0.043, 0.655, 0.778, and 0.202 Kobidil, Citrate, BTS and Tris, respectively. For rapid motility and osteopontin, Kolbroek boar semen revealed positive correlations of 0.842, 0.601, 0.164 and 0.202, respectively while large white boars semen showed correlations of −0.909, −0.144, −0.210, and 0.089, respectively. Only BTS and Kobidil showed high negative correlations between osteopontin and viability in Kolbroek (−0.975) and Large white (−0.746) boar semen, respectively. No significant correlations with osteopontin were observed when Tris extender was used in both breeds. In conclusion, Kolbroek boar semen requires osteopontin in Kobodil extender while large white boar semen requires osteopontin in BTS extender.

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“…In order to get more insemination doses from one ejaculate, boar semen is diluted with different diluents. The role of diluents is to reduce the metabolic activity of sperm, preserve its vitality and fertilization ability (Johnson et al, ______________________________________________________________________________ 66 2000), provide the necessary nutrients for the maintenance of sperm metabolism (Mapeka et al, 2012), maintain a pH of between 6.8-7.2, stabilize the plasma membrane, regulate the osmotic pressure to which spermatozoa are exposed, and prevent bacterial growth. Semen diluents mainly consist of glucose (as a source of energy), protective substances against the thermal shock (eg BSA, Bovine Serum Albumin), buffering salts (in order to avoid the negative effects of the pH change (sodium bicarbonate, sodium citrate, TRIS)), basic salts (that provide an appropriate osmotic balance (NaCl, KCl)) and antibiotics (to prevent bacterial growth) (Paquinon et al, 1988;Gadea et al, 2004;Gogol et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Effects of Antibiotic Diluent Additives on the Motility Parameters and Morphological Integrity of Boar Sperm During Six Days of Storage

Jotanović

Peno

Mandić

et al. 2019

Contemporary Agriculture

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Summary The purpose of this paper is to examine the effect of antibiotic diluent additives on the motility and morphological integrity of diluted fresh boar semen during a six-day storage period. A total of 60 insemination doses, originating from two Landrace boars, were examined and allocated to control (C, n=30, diluted with BTS) and experimental groups (E, n=30, diluted with BTS upon antibiotic addition). The treatment applied exerted positive effects on the preservation of progressive motility, percentage of live sperm and HOS test results (70.24 vs. 66.53%, 71.54 vs. 69.77%, 67.35 vs. 64.17% and 64.10 vs. 54.26%;91.15 vs. 90.02%, 88.38 vs. 85.55%, 81.50 vs. 76.13% and 74.53 vs. 68.72%; and 93.35 vs. 92.40%, 91.04 vs. 88.02 %, 84.67 vs. 78.15% and 77.27 vs. 69.44% HOS+ sperm for the 1st, 3rd, 5th and 6th day of storage, respectively). The results obtained indicate that the treatment applied has a favourable effect on preserving the quality parameters of diluted fresh boar semen during storage, resulting most likely from a reduction of bacterial contamination.

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Comparison of different extenders and storage temperature on the sperm motility characteristics of Kolbroek pig semen

Cited by 8 publications

References 12 publications

Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India

Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India

An Investigative Immunoassay Targeting Two Osteopontin Epitopes in Boar Semen

Effects of Antibiotic Diluent Additives on the Motility Parameters and Morphological Integrity of Boar Sperm During Six Days of Storage

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