Comparison of different in vitro mucoadhesion testing methods for hydrogels

Yan, Jun; Chen, Xiaojin; Yu, Shufei; Zhou, Hongping

doi:10.1016/j.jddst.2017.06.012

Cited by 11 publications

(4 citation statements)

References 37 publications

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“…The flowing liquid test was performed to investigate the mucoadhesive properties of the prepared formulations. This method involves washing the formulation with an appropriate artificial fluid at a constant flow rate while the residence time of the formulation is determined visually or fluorometrically [ 32 ]. The in vitro mucoadhesive test was conducted under a condition mimicking the pre-ocular surface.…”

Section: Methodsmentioning

confidence: 99%

Mucoadhesive Hybrid System of Silk Fibroin Nanoparticles and Thermosensitive In Situ Hydrogel for Amphotericin B Delivery: A Potential Option for Fungal Keratitis Treatment

Chomchalao,

Saelim,

Lamlertthon

et al. 2024

Polymers

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The purpose of this work was to investigate the feasibility of a novel ophthalmic formulation of amphotericin B-encapsulated silk fibroin nanoparticles incorporated in situ hydrogel (AmB-FNPs ISG) for fungal keratitis (FK) treatment. AmB-FNPs ISG composites were successfully developed and have shown optimized physicochemical properties for ocular drug delivery. Antifungal effects against Candida albicans and in vitro ocular irritation using corneal epithelial cells were performed to evaluate the efficacy and safety of the composite formulations. The combined system of AmB-FNPs-ISG exhibited effective antifungal activity and showed significantly less toxicity to HCE cells than commercial AmB. In vitro and ex vivo mucoadhesive tests demonstrated that the combination of silk fibroin nanoparticles with in situ hydrogels could enhance the adhesion ability of the particles on the ocular surface for more than 6 h, which would increase the ocular retention time of AmB and reduce the frequency of administration during the treatment. In addition, AmB-FNP-PEG ISG showed good physical and chemical stability under storage condition for 90 days. These findings indicate that AmB-FNP-PEG ISG has a great potential and be used in mucoadhesive AmB eye drops for FK treatment.

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Section: Methodsmentioning

confidence: 99%

Mucoadhesive Hybrid System of Silk Fibroin Nanoparticles and Thermosensitive In Situ Hydrogel for Amphotericin B Delivery: A Potential Option for Fungal Keratitis Treatment

Chomchalao,

Saelim,

Lamlertthon

et al. 2024

Polymers

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“…HPMC and Na-CMC were used alone in conjunction with appropriate quantity for the preparation of gels (Chen, Yan, Shuying, 2018;Yan et al, 2017). Table I demonstrate the composition of gel formulations with various polymers.…”

Section: Formulation Of Oral Mucoadhesive Bsp and Dsp Gelsmentioning

confidence: 99%

A comparative physicochemical and pharmacological evaluation of dexamethasone sodium phosphate and betamethasone sodium phosphate mucoadhesive gels for the treatment of oral submucous fibrosis in rats

Desai¹,

Shirsand²,

Surampalli³

2022

Braz. J. Pharm. Sci.

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The present study is aimed to formulate steroidal oral mucoadhesive gels of dexamethasone sodium phosphate and betamethasone sodium phosphate. Six gel formulations each of dexamethasone sodium phosphate and betamethasone sodium phosphate prepared using two different polymers carboxymethyl cellulose sodium and hydroxypropyl methylcellulose, in variable proportions. All the formulations subjected for assessment of various physicochemical parameters and mechanical properties. The formulations BSP 5 and DSP 5, both containing 1.25 % carboxymethyl cellulose sodium, 1.25 % hydroxypropyl methylcellulose, exhibiting mucoadhesive strength of 12.300 ± 0.004 and 12.600 ± 0.01, adhesiveness of 28.04 ± 00 and 30.02 ± 00, cohesiveness of 28.04 ± 00 and 30.02 ± 00, drug release of 86.869 ± 0.380 % and 88.473 ± 0.457 % respectively were considered as promising ones and were further subjected for stability studies and in vivo study in male albino rats. Formulation DSP 5 upon oral application for 4 months in arecoline induced oral submucous fibrosis rats, showed more than 80 % reduction in fibrosis as compared with BSP 5 which showed nearly 50 % reduction. These results were concluded on the basis of histopathological profile and weight gain among the experimental animals during in vivo study. Hence, DSP 5 by minimizing the painful injuries and morbidities justifies being suitable noninvasive model for OSMF treatment.

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“…In this context, the use of hydrogels seems to be a promising strategy, since it combines numerous advantages to wound management, e.g., enhancing the healing process [16,17], reducing pain [18], enabling exchange of gases (e.g., O 2 and H 2 O) [19], possibility to tailor the mucoadhesion [20], act as a barrier to external threats like microbes [19]. Moreover, it can, simultaneously, act as a carrier for therapeutic agents [21,22,23,24].…”

Section: Introductionmentioning

confidence: 99%

A Functional Wound Dressing as a Potential Treatment for Cutaneous Leishmaniasis

et al. 2019

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Cutaneous leishmaniasis (CL) is a parasitic disease characterized by progressive skin sores. Currently, treatments for CL are limited to parenteral administration of the drug, which presents severe adverse effects and low cure rates. Therefore, this study aimed to develop poly(vinyl-alcohol) (PVA) hydrogels containing Amphotericin B (AmB) intended for topical treatment of CL. Hydrogels were evaluated in vitro for their potential to eliminate promastigote forms of Leishmania spp., to prevent secondary infections, to maintain appropriate healing conditions, and to offer suitable biocompatibility. AmB was incorporated into the system in its non-crystalline state, allowing it to swell more and faster than the system without the drug. Furthermore, the AmB release profile showed a continuous and controlled behavior following Higuchi´s kinetic model. AmB-loaded-PVA-hydrogels (PVA–AmB) also showed efficient antifungal and leishmanicidal activity, no cytotoxic potential for VERO cells, microbial impermeability and water vapor permeability compatible with the healthy skin’s physiological needs. Indeed, these results revealed the potential of PVA–AmB to prevent secondary infections and to maintain a favorable environment for the healing process. Hence, these results suggest that PVA–AmB could be a suitable and efficient new therapeutic approach for the topical treatment of CL.

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Comparison of different in vitro mucoadhesion testing methods for hydrogels

Cited by 11 publications

References 37 publications

Mucoadhesive Hybrid System of Silk Fibroin Nanoparticles and Thermosensitive In Situ Hydrogel for Amphotericin B Delivery: A Potential Option for Fungal Keratitis Treatment

Mucoadhesive Hybrid System of Silk Fibroin Nanoparticles and Thermosensitive In Situ Hydrogel for Amphotericin B Delivery: A Potential Option for Fungal Keratitis Treatment

A comparative physicochemical and pharmacological evaluation of dexamethasone sodium phosphate and betamethasone sodium phosphate mucoadhesive gels for the treatment of oral submucous fibrosis in rats

A Functional Wound Dressing as a Potential Treatment for Cutaneous Leishmaniasis

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