Comparison of different label-free imaging high-throughput biosensing systems for aptamer binding measurements using thrombin aptamers

Rath, Christin; Burger, Jürgen; Norval, Leo; Kraemer, Stefan Daniel; Gensch, Nicole; Kooi, Alexander van der; Reinemann, Christine; O’Sullivan, Ciara K.; Svobodová, Markéta; Roth, Günter

doi:10.1016/j.ab.2019.05.012

Cited by 9 publications

(7 citation statements)

References 31 publications

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“…Details are given in [414]; company links are provided in [415]. The second study compares Biacore 3000, Octet RED96, IBIS MX96, and 2 imaging RIfS instruments [416]. Some instruments allow multiplex measurements (less than 100 spots in parallel), only for the imaging iRIfS more than 1000 spots can be measured in parallel.…”

Section: Detection Methods Reviewed and Comparedmentioning

confidence: 99%

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

Gauglitz

2020

Anal Bioanal Chem

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Direct optical detection has proven to be a highly interesting tool in biomolecular interaction analysis to be used in drug discovery, ligand/receptor interactions, environmental analysis, clinical diagnostics, screening of large data volumes in immunology, cancer therapy, or personalized medicine. In this review, the fundamental optical principles and applications are reviewed. Devices are based on concepts such as refractometry, evanescent field, waveguides modes, reflectometry, resonance and/or interference. They are realized in ring resonators; prism couplers; surface plasmon resonance; resonant mirror; Bragg grating; grating couplers; photonic crystals, Mach-Zehnder, Young, Hartman interferometers; backscattering; ellipsometry; or reflectance interferometry. The physical theories of various optical principles have already been reviewed in detail elsewhere and are therefore only cited. This review provides an overall survey on the application of these methods in direct optical biosensing. The "historical" development of the main principles is given to understand the various, and sometimes only slightly modified variations published as "new" methods or the use of a new acronym and commercialization by different companies. Improvement of optics is only one way to increase the quality of biosensors. Additional essential aspects are the surface modification of transducers, immobilization strategies, selection of recognition elements, the influence of non-specific interaction, selectivity, and sensitivity. Furthermore, papers use for reporting minimal amounts of detectable analyte terms such as value of mass, moles, grams, or mol/L which are difficult to compare. Both these essential aspects (i.e., biochemistry and the presentation of LOD values) can be discussed only in brief (but references are provided) in order to prevent the paper from becoming too long. The review will concentrate on a comparison of the optical methods, their application, and the resulting bioanalytical quality.

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Section: Detection Methods Reviewed and Comparedmentioning

confidence: 99%

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

Gauglitz

2020

Anal Bioanal Chem

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“…One obtains the association constant K A = 8 and 14 in Supplementary information) and/or spatial heterogeneity of the ligand sites to be approached by ligate which becomes significant as ligand sites approach saturation [14]. Furthermore, a study of 2019 found out that different measurement systems can deviate up to a factor of 20 for the same analyte [15]. Considering this, variations in determining K A with different setups are more than expectable.…”

Section: Binding Interaction Testmentioning

confidence: 99%

Fourier spotting: a novel setup for single-color reflectometry

et al. 2022

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Single-color reflectrometry is a sensitive and robust detection method in optical biosensor applications, for example for bioanalysis. It is based on the interference of reflected monochromatic radiation and is label free. We present a novel setup for single-color reflectometry based on the patented technology of Berner et al. from 2016. Tilting areas of micro-mirrors allow us to encode the optical reflection signal of an analyte and reference channel into a particular carrier frequency with the amplitude being proportional to the local reflection. Therefore, a single photodiode is sufficient to collect the signals from both channels simultaneously. A 180∘ phase shift in the tilt frequency of two calibrated micro-mirror areas leads to a superposition of the analyte and reference signal which enables an efficient reduction of the baseline offset and potential baseline offset drift. A performance test reveals that we are able to detect changes of the refractive index n down to Δn < 0.01 of saline solutions as regents. A further test validates the detection of heterogeneous binding interaction. This test compromises immobilized testosterone-bovine serum albumin on a three-dimensional layer of biopolymer as ligand and monoclonal anti-testosterone antibodies as analyte. Antibody/antigen binding induces a local growth of the biolayer and change in the refractive index, which is measured via the local change of the reflection. Reproducible measurements enable for the analysis of the binding kinetics by determining the affinity constant KA = 1.59 × 10− 7 M− 1. In summary, this work shows that the concept of differential Fourier spotting as novel setup for single-color reflectometry is suitable for reliable bioanalysis.

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“…Recently, biolayer interferometry (BLI) has been considered as one of the most promising techniques for optical fiber-based, label-free, and real-time detection of biomolecular interactions [ 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 ]. BLI includes two reflective surfaces forming an FPI created by a reference layer at an optical fiber tip and the surface of immobilized bioreceptors as shown in Figure 12 [ 90 ].…”

Section: Fabry-perot Interferometer (Fpi)-based Biosensorsmentioning

confidence: 99%

“…A detector measures the reflectance spectrum in real-time, enabling binding kinetics analyses. BLI has been employed in a wide range of biosensing applications from the study and development of drugs and vaccines [ 81 , 83 ] to the detection of various biomolecules [ 85 , 86 , 87 , 89 , 91 , 92 , 93 , 94 ].…”

Section: Fabry-perot Interferometer (Fpi)-based Biosensorsmentioning

confidence: 99%

“…The Octet platform (ForteBio, Fremont, CA, USA) is an another commercial BLI-based biosensor, which is capable of detecting biomolecules with a molecular weight of 150 Da, using up to 384-wells for high throughput [ 79 , 82 , 86 , 95 ]. The LOD given by the manufacturer falls in the range between nM and μM [ 86 ]. Gao et al reported the detection of Gonyautoxin 1/4 (GTX1/4) aptamer with an Octet platform [ 92 ].…”

Section: Fabry-perot Interferometer (Fpi)-based Biosensorsmentioning

confidence: 99%

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Label-Free Optical Resonator-Based Biosensors

Rho

Breaux

Kim

2020

Sensors

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The demand for biosensor technology has grown drastically over the last few decades, mainly in disease diagnosis, drug development, and environmental health and safety. Optical resonator-based biosensors have been widely exploited to achieve highly sensitive, rapid, and label-free detection of biological analytes. The advancements in microfluidic and micro/nanofabrication technologies allow them to be miniaturized and simultaneously detect various analytes in a small sample volume. By virtue of these advantages and advancements, the optical resonator-based biosensor is considered a promising platform not only for general medical diagnostics but also for point-of-care applications. This review aims to provide an overview of recent progresses in label-free optical resonator-based biosensors published mostly over the last 5 years. We categorized them into Fabry-Perot interferometer-based and whispering gallery mode-based biosensors. The principles behind each biosensor are concisely introduced, and recent progresses in configurations, materials, test setup, and light confinement methods are described. Finally, the current challenges and future research topics of the optical resonator-based biosensor are discussed.

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Comparison of different label-free imaging high-throughput biosensing systems for aptamer binding measurements using thrombin aptamers

Cited by 9 publications

References 31 publications

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

Fourier spotting: a novel setup for single-color reflectometry

Label-Free Optical Resonator-Based Biosensors

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