Comparison of different methods of centrifugation for preparation of platelet-rich plasma (PRP)

Bhatia, Akanksha; Ramya, BS; Biligi, Dayananda S; Bk, Panchakshari Prasanna

doi:10.5958/2394-6792.2016.00099.5

Cited by 9 publications

(6 citation statements)

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“…In the study conducted by Bhatia et al, the single spin method demonstrated a platelet concentration factor of 2.19, while the double spin method resulted in a reduction in platelet counts (platelet concentration factor=0.83). Our study also demonstrated similar findings, therefore it is safe to conclude that the single centrifugation method surpasses the double centrifugation method in preparing platelet-rich plasma, as indicated by the higher platelet concentration factor [28].…”

Section: Discussionsupporting

confidence: 82%

The Efficacy of Local Autologous Platelet-Rich Plasma Prepared by Single and Double Spin Methods in the Treatment of Chronic Ulcer

Dubey,

Raghuwanshi

2024

Cureus

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Background: Chronic nonhealing ulcers present significant challenges in diabetic, dermatological, and surgical patients. Platelet-rich plasma (PRP), enriched with bioactive factors, offers promise for wound healing enhancement. This study evaluates PRP's efficacy, prepared via single and double spin methods in nonhealing chronic ulcers.Methods: Twenty-two patients aged 18-65 years participated and 100 mL of blood was drawn into citrate phosphate dextrose adenine (CPDA) bags with all aseptic precautions. PRP was prepared by single and double spin methods. Patient serum and 10% calcium gluconate were added to fibrin gel. PRP was injected around the ulcer and then dressed. Dressings were changed on the fifth, 15th, and 20th days with PRP. Evaluation occurred on day 30 using surface area and volume assessments by both methods.Results: The single spin PRP group and double spin PRP group had 11 patients each with hemoglobin range of 10.79±1.88 to 12.63±2.22 g/dL. Initial lesions (16.27 cm²) significantly reduced to 14.76 cm² after double spin PRP sessions (p=0.005) and Initial lesions (9.87 cm²) significantly reduced to 7.65 cm² after single spin PRP sessions (p=0.005). Platelet count differences between whole blood and PRP were significant (p<0.05).Conclusions: The single spin PRP method exhibited considerable improvements in healing parameters, showcasing its potential for chronic ulcer management.

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Section: Discussionsupporting

confidence: 82%

The Efficacy of Local Autologous Platelet-Rich Plasma Prepared by Single and Double Spin Methods in the Treatment of Chronic Ulcer

Dubey,

Raghuwanshi

2024

Cureus

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“…There is no standard method of preparation of PRP in literature till now. In the present study, we performed a single centrifugation spin with a speed of 3500 round per minute for 15 min, because it was demonstrated that a single centrifugation method yielded a better platelet count as compared to a double centrifugation method 14 …”

Section: Discussionmentioning

confidence: 99%

Evaluation of safety and efficacy of autologous topical platelet gel vs platelet rich plasma injection in the treatment of venous leg ulcers: A randomized case control study

Elgarhy

El-Ashmawy

Bedeer

et al. 2020

Dermatologic Therapy

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Various management options were tried for Chronic venous leg ulcers (VLUs) with no satisfactory results. Platelet-rich plasma (PRP) is promising in enhancing chronic wound healing by releasing various growth factors. We evaluated the safety and efficacy of topical platelet gel (PG) vs PRP injection in treatment of venous leg ulcers. 20 patients were treated with topical PG (group I), 20 patients received PRP injection (group II), and 20 patients were treated with saline dressing and compression as controls (group III) weekly for 6 weeks. Ulcer improvement was evaluated by pressure ulcer scale for healing (PUSH). Histopathological evaluation and immunohistochemical staining using anti-CD34 were performed before and after treatment. There was significant improvement in PUSH score in group (I) and (II) compared to group (III; P value: .001). However, there was no statistically significant difference in improvement between group I and II (P value = .83). However, topical PG was more effective in diabetics and PRP was more effective in long standing fibrosed ulcers. Histopathology showed reduction of the inflammatory cells infiltrates and well-formed granulation tissue with no difference in immunostaining. So, both topical PG and PRP injection were effective, low cost, and safe procedures in enhancing healing of chronic VLUs.

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“…The baseline investigations such as complete hemogram, ESR, CRP, renal function tests, random blood glucose, serological testing for HIV 1 & 2 and HbsAg and radiographic analysis of affected knee joint are done. Preparation of autologous platelet rich plasma injection 3 The method by which autologous PRP prepared is called differential centrifugation. 20 ml of autologous venous blood are withdrawn into test tubes containing sodium citrate and are subjected first centrifugation of 3000 rpm for 15 minutes.…”

Section: Methodsmentioning

confidence: 99%

The efficacy of autologous platelet rich plasma vs homologous platelet lysate in patients with early knee osteoarthritis

Jeyaraman¹,

Ramesh²,

Gs³

2020

IJOR

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Introduction: Recently the idea of "Orthobiologics" leads to development of less invasive procedures and accelerated treatments which enhances functional recovery of musculoskeletal disorders. The desired therapeutic effect of autologous platelet rich plasma is facilitated by the ability of IL-1-Ra to limit the destructive inflammatory intra-articular (IA) actions of IL-1β. Objectives: To evaluate the efficacy and functional outcome of autologous PRP injection and homologous platelet lysate to reduce pain, improve joint function and enhance quality of life in patients with early knee osteoarthritis and to investigate the advantages and disadvantages of PRP and homologous platelet lysate. Material and Methods: A total of 121 cases of early osteoarthritis were equally divided into PRP and homologous platelet lysate group. Each patient in both groups has been given ultrasound guided PRP and homologous platelet lysate on day 0, at the end of 4 weeks and 8 weeks and all the patients were followed for up to one year. Treatment outcome measures were assessed at each visit using VAS and WOMAC scores. Results: A statistically significant improvement is observed with VAS and WOMAC score in knee osteoarthritis patients over 12 months with greater advantages over homologous platelet lysate. The overall reduction of pain and enhanced joint function was observed within 1 week, 3 months and 12 months after the initial injection. Conclusion:Homologous platelet lysate has the potential to offer chondroprotective and molecular approach in treating pain and functionality in patients with mild and moderate knee osteoarthritis with greater advantages over autologous platelet rich plasma.

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Comparison of different methods of centrifugation for preparation of platelet-rich plasma (PRP)

Cited by 9 publications

References 0 publications

The Efficacy of Local Autologous Platelet-Rich Plasma Prepared by Single and Double Spin Methods in the Treatment of Chronic Ulcer

The Efficacy of Local Autologous Platelet-Rich Plasma Prepared by Single and Double Spin Methods in the Treatment of Chronic Ulcer

Evaluation of safety and efficacy of autologous topical platelet gel vs platelet rich plasma injection in the treatment of venous leg ulcers: A randomized case control study

The efficacy of autologous platelet rich plasma vs homologous platelet lysate in patients with early knee osteoarthritis

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