Comparison of different needle diameters and flow rates on bone marrow mononuclear stem cell viability: an ex vivo experimental study

Tol, Meltem; Akar, Ahmet; Durdu, Serkan; Ayyildiz, Erol; İlhan, Osman

doi:10.1080/14653240701762356

Cited by 19 publications

(23 citation statements)

References 5 publications

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“…To facilitate the translation of cell therapy from bench to bedside, numerous studies have been carried out to assess the effects of the injection process on cell functionality during and after cell delivery. However, a range of needle sizes have been used in injectability studies, complicating comparisons [13,15–19]. This has led to conflicting results, with studies showing cell manipulation through a needle affected cell viability,[17] and others stating that flow through a needle did not have a significant effect [19].…”

Section: Introductionmentioning

confidence: 99%

The effect of injection using narrow-bore needles on mammalian cells: administration and formulation considerations for cell therapies

Amer

White

Shakesheff

2015

Journal of Pharmacy and Pharmacology

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ObjectivesThis study focuses on the effect of the injection administration process on a range of cell characteristics.MethodsEffects of different ejection rates, needle sizes and cell suspension densities were assessed in terms of viability, membrane integrity, apoptosis and senescence of NIH 3T3 fibroblasts. For ratiometric measurements, a multiplex assay was used to verify cell viability, cytotoxicity and apoptosis independent of cell number. Co‐delivery with alginate hydrogels and viscosity‐modifying excipients was also assessed.Key findingsEjections at 150 μl/min resulted in the highest percentage of dose being delivered as viable cells among ejection rates tested. The difference in proportions of apoptotic cells became apparent 48 h after ejection, with proportions being higher in samples ejected at slower rates. Co‐delivery with alginate hydrogels demonstrated a protective action on the cell payload.ConclusionsThis study demonstrates the importance of careful consideration of administration protocols required for successful delivery of cell suspensions, according to their nature and cellular responses post‐ejection.

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Section: Introductionmentioning

confidence: 99%

The effect of injection using narrow-bore needles on mammalian cells: administration and formulation considerations for cell therapies

Amer

White

Shakesheff

2015

Journal of Pharmacy and Pharmacology

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“…Also it is necessary to quantitatively study the effect of shear during injection on hydrogel flow behavior as the entry of materials into, and flow of materials through, a capillary geometry is known to cause significant amounts of shear and deformation in a material depending on the material properties. In addition, the application of mechanical shear on cells can significantly change cell biological behavior 16,17,44-50 . It is important to state that while some cells are normally exposed to high shear flow ( e.g.…”

Section: Introductionmentioning

confidence: 99%

Injectable Solid Peptide Hydrogel as a Cell Carrier: Effects of Shear Flow on Hydrogels and Cell Payload

et al. 2012

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β-hairpin peptide-based hydrogels are a class of injectable solid hydrogels that can deliver encapsulated cells or molecular therapies to a target site via syringe or catheter injection as a carrier material. These physical hydrogels can shear-thin and consequently flow as a low-viscosity material under a sufficient shear stress but immediately recover back into a solid upon removal of the stress, allowing them to be injected as preformed gel solids. Hydrogel behavior during flow was studied in a cylindrical capillary geometry that mimicked the actual situation of injection through a syringe needle in order to quantify effects of shear-thin injection delivery on hydrogel flow behavior and encapsulated cell payloads. It was observed that all β-hairpin peptide hydrogels investigated displayed a promising flow profile for injectable cell delivery: a central wide plug flow region where gel material and cell payloads experienced little or no shear rate and a narrow shear zone close to the capillary wall where gel and cells were subject to shear deformation. The width of the plug flow region was found to be weakly dependent on hydrogel rigidity and flow rate. Live-dead assays were performed on encapsulated MG63 cells three hours after injection flow and revealed that shear-thin delivery through the capillary had little impact on cell viability and the spatial distribution of encapsulated cell payloads. These observations help us to fundamentally understand how the gels flow during injection through a thin catheter and how they immediately restore mechanically and morphologically relative to pre-flow, static gels.

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“…Numerous studies have analyzed various factors which could affect the cell viability and other parameters during and after cell delivery [1,20]. Many of the methods of cell delivery require the use of syringes to deliver the cells to the appropriate site, for instance, multiple injections are made to the left ventricular myocardium when treating heart failure due to ischemic disease.…”

Section: Introductionmentioning

confidence: 99%

Impact of passing mesenchymal stem cells through smaller bore size needles for subsequent use in patients for clinical or cosmetic indications

Mamidi

Singh²,

Husin³

et al. 2012

J Transl Med

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BackgroundNumerous preclinical and clinical studies have investigated the regenerative potential and the trophic support of mesenchymal stem cells (MSCs) following their injection into a target organ. Clinicians favor the use of smallest bore needles possible for delivering MSCs into vascular organs like heart, liver and spleen. There has been a concern that small needle bore sizes may be detrimental to the health of these cells and reduce the survival and plasticity of MSCs.MethodsIn this report, we aimed to investigate the smallest possible bore size needle which would support the safe delivery of MSCs into various tissues for different clinical or cosmetic applications. To accomplish this we injected cells via needle sizes 24, 25 and 26 G attached to 1 ml syringe in the laboratory and collected the cells aseptically. Control cells were ejected via 1 ml syringe without any needle. Thereafter, the needle ejected cells were cultured and characterized for their morphology, attachment, viability, phenotypic expression, differentiation potential, cryopreservation and in vivo migration abilities. In the second phase of the study, cells were injected via 26 G needle attached to 1 ml syringe for 10 times.ResultsSimilar phenotypic and functional characteristics were observed between ejected and control group of cells. MSCs maintained their cellular and functional properties after single and multiple injections.ConclusionsThis study proves that 26 G bore size needles can be safely used to inject MSCs for clinical/therapeutics purposes.

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Comparison of different needle diameters and flow rates on bone marrow mononuclear stem cell viability: an ex vivo experimental study

Cited by 19 publications

References 5 publications

The effect of injection using narrow-bore needles on mammalian cells: administration and formulation considerations for cell therapies

The effect of injection using narrow-bore needles on mammalian cells: administration and formulation considerations for cell therapies

Injectable Solid Peptide Hydrogel as a Cell Carrier: Effects of Shear Flow on Hydrogels and Cell Payload

Impact of passing mesenchymal stem cells through smaller bore size needles for subsequent use in patients for clinical or cosmetic indications

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