Comparison of Different Preconditioning and Loading Treatments With Vanilla (Vanilla Planifolia Jack.) Apices Cryopreserved Using the Droplet-Vitrification Procedure

Hernández-Ramírez, Fabiola; Gonzalez-Arnao, Maria Teresa; Cruz‐Cruz, Carlos A.; Pastelín-Solano, Miriam Cristina; Engelmann, Florent

doi:10.17660/actahortic.2014.1039.22

Cited by 8 publications

(1 citation statement)

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“…In orchids, cryopreservation has been an efficient means of conserving seeds and pollen (e.g., Dolce et al 2016;Flachsland et al 2006;Hay et al 2010;Koopowitz 1986;Koopowitz and Thornhill, 1994;Mweetwa et al 2007;Popov et al 2004;Pritchard 1984;Vendrame et al 2007b;Surenciski et al 2012). By contrast, attempts to cryopreserve somatic explants (i.e., clonal material) are scarce and have resulted in variable regrowth González-Arnao et al 2009;Hernández-Ramírez et al 2014;Kondo et al 2001;Lurswijidjarus and Thammasiri 2004;Na and Kondo 1996;Thinh and Takagi 2000;Tsukazaki et al 2000).…”

Section: In Vitro Conservation Approaches In Orchidsmentioning

confidence: 99%

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Dolce

Medina

Terada

et al. 2020

Orchid Biology: Recent Trends &Amp; Challenges

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Orchids are an important part of plant biodiversity on the planet due to their high variability among species and their habitats. South America presents more than thirty percent of all known orchid species, being Colombia, Ecuador, Brazil, Peru, and Bolivia between the richest countries in the world in terms of orchid biodiversity. Nevertheless, concerning to the orchid conservation status, in Colombia precisely orchids occupy the unlucky first place as the plant family with the highest number of threatened species. Similar situation is happening in the rest of the South American countries. The two main threats to orchid survival are both anthropogenic: the first one is deforestation, and the second largest threat to orchids is collection from the wild. One desirable action to safeguard these endangered species is to develop procedures that make possible their massive propagation, which should provide material for the eco-rehabilitation of specimens into their natural habitats, the exchange with other entities, the supply to orchid merchants for avoid extractions of nature, and the availability of material for future research. Likewise, the development of systems that allow the ex situ conservation of orchid germplasm is imperative. This chapter reviews the progresses of different in vitro approaches for orchid propagation and germplasm conservation, safeguarding the genetic biodiversity of these species. Several study cases are presented and described to exemplify the protocols developed in the Botanical Institute of Northeast (UNNE-CONICET) for propagating and long-term storing the germplasm of wild orchids from Argentina (Cattleya lundii, Cohniella cepula, Cohniella jonesiana, Gomesa bifolia, Aa achalensis, Cyrtopodium brandonianum, Cyrtopodium hatschbachii, Habenaria bractescens). Moreover, it has been attempted to put together most of the available literature on in vitro propagation and germplasm conservation for South American orchids using different explants and procedures. There are researches of good scientific quality that even cover critical insights into the physiology and factors affecting growth and development as well as storage of several orchid materials. Anyway, studies are still necessary to cover a mayor number of South American species as well as the use of selected material (clonal) for both propagation and conservation approaches.

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