Comparison of different wound healing assays performed with lupeol-treated human keratinocytes

Werner, Peter; Wardecki, Tina; Madl, James E.; Nascimento, Jadiely do; Römer, Winfried; Börries, Melanie; Merfort, Irmgard

doi:10.1055/s-0035-1565621

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“…Lupeol is a triterpenoid that is known for its anti‐inflammatory efficacy, and its anti‐aging effect is yet to be clarified , but some authors have reported that lupeol affects cell cycle control via the p53 pathway and is attributed to apoptosis . Also, lupeol makes it possible to control keratinocyte proliferation, and it can therefore be used in anti‐inflammatory compositions . However, the anti‐aging efficacy and molecular mechanisms of lupeol have not been reported so far.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Lupeol on MMPs Expression using Aged Fibroblast through Repeated UVA Irradiation

Park

2018

Photochem & Photobiology

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In this study, the aged dermal fibroblast model was constructed by repeated irradiation with UV light and the effect of lupeol, a triterpenoid, on anti‐aging was confirmed. SA‐β‐galactosidase (SA‐β‐gal) stained aged cells increased by about 40% and expression of p‐p53, p21, p16 and MMPs (MMP‐1, ‐2, ‐3) increased in aged fibroblast. As an efficacy result, the treatment of lupeol on aged fibroblast induced by UVA repeated irradiation showed a dose‐dependent reduction of SA‐β‐gal stained aged cells, the expression of p‐p53, p21, p16 and inhibition of MMPs. Interestingly, lupeol increased dephosphorylation of p‐ERK in repeated UV irradiated conditions. Additionally, lupeol compensated MMPs expression when p‐ERK phosphorylation was inhibited by p‐ERK inhibitor PD98059. Thus, these results showed that lupeol has a possible effect on MMPs expression using inhibition of the p‐ERK pathway. Taken together, we confirmed that lupeol inhibits senescence through inhibiting MMP‐1, ‐2, ‐3 as well as p‐p53, p21 and p16 expression and SA‐β‐gal activity in repeated UVA‐irradiated senescent FB models, therefore suggesting that lupeol may be useful as an anti‐aging agent.

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Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Lupeol on MMPs Expression using Aged Fibroblast through Repeated UVA Irradiation

Park

2018

Photochem & Photobiology

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show abstract

Comparison of different wound healing assays performed with lupeol-treated human keratinocytes

Cited by 1 publication

References 0 publications

Inhibitory Effect of Lupeol on MMPs Expression using Aged Fibroblast through Repeated UVA Irradiation

Inhibitory Effect of Lupeol on MMPs Expression using Aged Fibroblast through Repeated UVA Irradiation

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