Comparison of differential expression genes in ovaries and testes of Pearlscale angelfish Centropyge vrolikii based on RNA-Seq analysis

Zhong, Zhaowei; Ao, Lulu; Wang, Yilei; Wang, Shuhong; Zhao, Liping; Ma, Senwei; Jiang, Yonghua

doi:10.1007/s10695-021-00977-y

Cited by 10 publications

(7 citation statements)

References 63 publications

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“…The results of in situ hybridization showed that when the gonads turned to the pure testis, the ovarian cells were not present, and the Cv‐Amh signals were more densely distributed in SCs than in germ cells. The sign of gonadal reversal beginning of C. Vrolikii is the appearance of male germ cells in the ovary, followed by the gonad further differentiating, and eventually transforming into pure testis (Zhong et al ., 2021). These results showed that Cv ‐ Amh is male‐biased, especially a test is biased gene.…”

Section: Discussionmentioning

confidence: 99%

“…At present, most C. vrolikii come from wild fishing, and artificial breeding has not yet broken through, which has become the restriction factor in meeting the needs of the market (Zhong et al ., 2021). Previously, the authors used RNA‐Seq technology to screen the differentially expressed genes (DEG) in C. vrolikii gonads at various stages (Zhong et al ., 2021), and the pluripotent transcription factor Nanog was cloned and analysed (Zhong et al ., 2022). The regulatory network of Amh has been extensively studied in mammals, but the molecular mechanism of the regulatory network in C. vrolikii has not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Expression pattern analysis ofanti‐Mullerianhormone in testis development of pearlscale angelfish (Centropyge vrolikii)

Zhong

Feng

et al. 2023

Journal of Fish Biology

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In vertebrates, anti-Mullerian hormone (Amh) secreted by Sertoli cells (SC) performs a pivotal function in male sex differentiation. Compared with that of higher vertebrates, the expression pattern of Amh is more diversified in fish. In this study, the full-length complementary DNA (cDNA) of Amh in Centropyge vrolikii (Cv-Amh) was cloned and analysed, which was 2,470 bp, including a 238 bp 5'UTR, a 1,602 bp ORF and a 633 bp 3'UTR; the similarity of Amh between Cv-Amh and other fish is relatively high. The quantitative real-time PCR (qRT-PCR) results of healthy tissues and gonads at sex reversal stages in C. vrolikii showed that the expression level of Amh in the testis was significantly higher than that in other tissues (P < 0.05). Amh was weakly expressed in the vitellogenic stage ovary and perinucleolus stage ovary, but its expression significantly increased in the gonads at the hermaphroditic stage, and finally reached the highest in the pure testis after sexual reversal. The results of in situ hybridization indicated that the positive signal of Amh was strongly concentrated in SCs of testis. After Amh knockdown in the gonads, the effect on sex-related genes was tested using qRT-PCR. Among these, the expression of Dmrt1, Cyp11a, Hsd11b2, Sox8 and Sox9 significantly decreased, whereas that of Cyp19a, Sox4, Foxl2 and Sox3 increased. These results suggested that Amh could be the pivotal gene in reproductive regulation in C. vrolikii, and the data will contribute to sex-related research of C. vrolikii in the future.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression pattern analysis ofanti‐Mullerianhormone in testis development of pearlscale angelfish (Centropyge vrolikii)

Zhong

Feng

et al. 2023

Journal of Fish Biology

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“…In medaka, the expression of Nanog was exhibited in the gonads, predominantly in pluripotent cells (Camp et al, 2009. C. vrolikii is a naturally reversed sh, the mark of the beginning of the gonad reversal is the male germ cells beginning to appear in the ovary,named hermaphrodite, and then the male germ cells are further differentiated, nally the gonad was transformed into pure testis (Zhong et al, 2021). In this study, there were differences in the expression of Cv-Nanog in gonadal tissues at different developmental stages with the level O > O > O T > T in turn (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…At present, C. vrolikii is obtained mainly by wild shing, but due to the increase of market demand and the destruction of ecological environment, the natural yield of C. vrolikii decreases year by year, and the arti cial breeding is inevitable. However, as a kind of protogynous hermaphrodite and natural sexual reversion sh, the disadvantages of long breeding cycle and di cult arti cial breeding become the main restricting factor for the breeding of C. vrolikii (Zhong et al, 2021). Therefore, it is indispensable to study the molecular regulation mechanism of gonadal development in C. vrolikii.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, it is indispensable to study the molecular regulation mechanism of gonadal development in C. vrolikii. In our previous research, the differential expression genes (DEGs) in gonads at different stages in C. vrolikii had been screened using RNA-Seq technology (Zhong et al, 2021). About Nanog gene of C. vrolikii, which we have designated as Cv-Nanog, very little research has been done at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the Nanog gene involved in the gonadal development in pearlscale angelfish (Centropyge vrolikii)

Zhong

Feng

et al. 2022

Fish Physiol Biochem

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The homeodomain transcription factor Nanog plays crucial roles in the embryonic/gonadal development and maintenance of embryonic stem cells (ESCs), interacting with transcription factors such as Oct4 and Sox2 in mammals. Nevertheless, knowledge of its exact pathways to molecular mechanism remains weak in teleosts, and unexploited in Pearlscale angel sh Centropyge vrolikii. The role of Nanog in the gonadal development and sex reversal of C. vrolikii is worth focusing. In this study, we isolated and characterized a Nanog homolog in C. vrolikii named as Cv-Nanog to understand the expression pattern of the gonadal development. The full-length cDNA sequence of Cv-Nanog was 2,136 bp in length and encodes a homeodomain protein of 436 amino acid residues. The analysis of phylogeny, the gene structure and western blot provided the evidence that Cv-Nanog was homologous to Nanog gene of mammalian. The protein sequence comparison demonstrated that Cv-Nanog shared the high similarity with orthologs of other vertebrates in the conserved homeodomain. The analysis of quantitative real-time PCR (qRT-PCR) and western blot showed that Cv-Nanog was maternally expressed detectable merely in gonads, and the expression was signi cantly high in ovaries than in testis. In situ hybridization revealed that the transcripts were located in the cytoplasm and membrane of the oocytes in ovaries, and in the testis, Cv-Nanog mRNA expression was weakly expressed in sertoli cells, but highly expressed in germ cells. After overexpression of Cv-Nanog, the expression levels of pluripotent factors Sox2 and Oct4 increased signi cantly with 21.5-fold and 12.2-fold, respectively. At the same time, the TGF-beta signaling pathway was activated, and the gonadal cells' growth was promoted, and the expression of ovary-bias genes Cyp19a and Foxl2 was up-regulated, the expression of testis-bias genes Sox9 and Dmrt1 was down-regulated, and nally the ovarian development was promoted. These results implied that Nanog gene may play a crucial role in the process of gonadal development and sexual reversion in C. vrolikii. This study is helpful to further understand the molecular regulatory mechanism of Nanog, also provides important clues for the future studies in gonadal development. HighlightsThe Cv-Nanog was signi cantly expressed in the gonads.The expression level of Cv-Nanog in the ovaries was signi cantly higher than that in the testis.The functional domain of Cv-Nanog was highly conserved among teleosts.In situ hybridization revealed that the transcript was located in the germ cells in the gonads.The TGF-beta signaling pathway was activated, and the gonadal cells' growth was promoted after overexpression Cv-Nanog in the gonads.

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Screening and expression analysis of genes related to skin coloration in pearlscale angelfish (Centropyge vrolikii)

Yang

Zhong

et al. 2023

Aquacult Int

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Comparison of differential expression genes in ovaries and testes of Pearlscale angelfish Centropyge vrolikii based on RNA-Seq analysis

Cited by 10 publications

References 63 publications

Expression pattern analysis ofanti‐Mullerianhormone in testis development of pearlscale angelfish (Centropyge vrolikii)

Expression pattern analysis ofanti‐Mullerianhormone in testis development of pearlscale angelfish (Centropyge vrolikii)

Characterization of the Nanog gene involved in the gonadal development in pearlscale angelfish (Centropyge vrolikii)

Screening and expression analysis of genes related to skin coloration in pearlscale angelfish (Centropyge vrolikii)

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