2023
DOI: 10.1016/j.cca.2023.117239
|View full text |Cite
|
Sign up to set email alerts
|

Comparison of digital PCR systems for the analysis of liquid biopsy samples of patients affected by lung and colorectal cancer

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

0
3
0

Year Published

2023
2023
2024
2024

Publication Types

Select...
7
1

Relationship

1
7

Authors

Journals

citations
Cited by 13 publications
(3 citation statements)
references
References 15 publications
0
3
0
Order By: Relevance
“…cfDNA was isolated from 3 ml of plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen, Valencia, CA). The analysis of IDH1 p.R132H mutation was performed by the QX200 digital droplet PCR (Bio-Rad, Hercules, CA) as previously reported [ 59 ].…”
Section: Methodsmentioning
confidence: 99%
“…cfDNA was isolated from 3 ml of plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen, Valencia, CA). The analysis of IDH1 p.R132H mutation was performed by the QX200 digital droplet PCR (Bio-Rad, Hercules, CA) as previously reported [ 59 ].…”
Section: Methodsmentioning
confidence: 99%
“…This platform has been shown to have similar performance to NGS [202]. A comparison between two PCR-based systems (ddPCR-BioRad and Qiagen QIAcuity Digital PCR) for the liquid biopsy analysis of cfDNA in a clinical study of NSCLC patients showed that solid digital PCR was generally more sensitive, and KRAS and EGFR mutations were detected with differing sensitivities [207].…”
Section: Multiplex-hybridization-based and Pcr-based Methodsmentioning
confidence: 99%
“…Following ampli cation, the uorescent signals emitted by these compartments are detected and analyzed, allowing researchers to calculate the concentration or copy number of the target molecule based on the Poisson distribution. This method provides absolute quanti cation of nucleic acids in a sample, making it a powerful tool in medical research, especially in oncology, where it is applied to analyze genetic variations in cancers such as breast and colorectal [10][11][12] . In our study, after sequencing the ovarian cancer cell line SKOV3/DDP, we detected eccDNA encoded by WWP1.…”
Section: Introductionmentioning
confidence: 99%