Comparison of direct fluorescent antibody staining and real-time reverse transcriptase PCR for the detection of novel 2009 influenza A (H1N1) in nasopharyngeal samples

Berg, Benjamin R.; Newton, Duane W.; LeBar, William

doi:10.1016/j.jcv.2011.06.002

Cited by 1 publication

(1 citation statement)

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“…Serological methods are much faster and less costly than viral culture methods, but may have a lower sensitivity and specificity compared with the culture methods described above. Immunofluorescence tests (IF), which enable the identification of antigens of a specific type of influenza virus in clinical samples are used frequently (Kapelusznik et al, 2009;Berg et al, 2011;). The method utilizes specific antibody labeled with a fluorescent dye such as fluorescein which binds to a particular antigen of influenza virus type A or B.…”

Section: Methods For Detection Of Influenza Virus Antigensmentioning

confidence: 99%

Influenza diagnosis and vaccination in Poland

Brydak

Woźniak-Kosek

Nitsch–Osuch

2013

Respiratory Physiology & Neurobiology

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In Poland between several thousand and several million cases of influenza and suspected influenza cases are registered, depending on the epidemic season. A variety of methods are available for the detection of the influenza viruses responsible for respiratory infection starting with the isolation of the virus in chick embryos or in cell lines such as MDCK, VERO, etc., and finishing with a variety of modifications of the classical PCR molecular biology such as PCR multiplex and Real-Time. The most effective way to combat influenza is through vaccination. Regular vaccination is one of the few steps that may be taken to protect individuals, especially in high-risk groups, from the potential and serious complications of influenza. In many countries, including Poland, despite the recommendations, the rate of vaccination against influenza is still low in all age groups. In the epidemic season 2011/2012, the level of distribution of the seasonal influenza vaccines was 4.5% of the population.

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Section: Methods For Detection Of Influenza Virus Antigensmentioning

confidence: 99%