Comparison of Direct Microscopy, Culture and Polymerase Chain Reaction Methods for the Diagnosis of Cutaneous Leishmaniasis

Ertabaklar, Hatice; S, Özlem Çalışkan; Boduç, Erengül; Ertuğ, Sema

doi:10.5578/mb.8344

Cited by 11 publications

(9 citation statements)

References 10 publications

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“…The methods include direct microscope test, culture of aspirates and histopathology biopsies. Although the specificity of these methods is high, varying from 86% [1] to 100% [2], they have important drawbacks mainly related to their sensitivity and time consuming due to the high subjective component.…”

Section: Introductionmentioning

confidence: 99%

Test accuracy of polymerase chain reaction methods against conventional diagnostic techniques for Cutaneous Leishmaniasis (CL) in patients with clinical or epidemiological suspicion of CL: Systematic review and meta-analysis

et al. 2020

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Background Molecular diagnostic tests, notably polymerase chain reaction (PCR), are highly sensitive test for Leishmania detection, which is especially relevant in chronic cutaneous lesion with lower parasite load. An accurate diagnosis is essential because of the high toxicity of the medications for the disease. Nevertheless, diagnosis of cutaneous leishmaniasis (CL) is hampered by the absence of a reference standard. Assuming that the PCR-based molecular tools are the most accurate diagnostic method, the objective of this systematic review was to assess the diagnostic accuracy of PCR-based molecular tools in a meta-analysis of the published literature. Methodology/Principal findings A search of the published literature found 142 papers of which only 13 studies met the selection criteria, including conventional PCR, real-time PCR, Loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), polymorphism-specific PCR (PS-PCR). The sensitivities of the individual studies ranged from 61% to 100%, and specificities ranged from 11% to 100%. The pooled sensitivities of PCR in smears were 0.95 (95% CI, 0.90 to 0.98), and the specificity was 0.91(95% CI, 0.70 to 0.98). In general population, estimates were lower in aspirates, skin biopsies and swab samples with 0.90 (95% CI, 0.80 to 0.95) and 0.87 (95% CI, 0.76 to 0.94) for sensitivity and specificity, respectively. The specificity was lower in consecutive studies, at 0.88 (95% CI, 0.59 to 0.98) and its CI were wider.

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Section: Introductionmentioning

confidence: 99%

Test accuracy of polymerase chain reaction methods against conventional diagnostic techniques for Cutaneous Leishmaniasis (CL) in patients with clinical or epidemiological suspicion of CL: Systematic review and meta-analysis

et al. 2020

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“…While there are multiple tests available for the diagnosis of VL, few of them can be used rapidly, without sophisticated laboratory equipment, or by individuals lacking technical training (Baneth and Aroch 2008; Grimaldi et al 2012; Mukhtar et al 2015). Traditionally, direct diagnosis achieved through microscopy has been the predominant means of Leishmania evaluation in field settings, but accurate microscopic diagnosis requires training and sensitivity is limited (Ertabaklar et al 2015; Santos et al 2014). Molecular assays, including direct detection of parasites by quantitative polymerase chain reaction (qPCR) or indirect methods such as immunofluorescence anti- Leishmania antibody test (IFAT) have been used at regional health centers but are not amenable to use in primary care clinics or true field settings (Ejazi and Ali 2013; Srividya et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Semi-quantitative measurement of asymptomatic L. infantum infection and symptomatic visceral leishmaniasis in dogs using Dual-Path Platform® CVL

Larson

Toepp

Scott

et al. 2016

Appl Microbiol Biotechnol

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Infection with Leishmania causes diseases with variable presentation. The most severe form is visceral leishmaniasis (VL), caused by either L. donovani or L. infantum. Despite efforts to eliminate VL, to date, molecular detection in resource-poor settings have lacked the accuracy and rapidity that would enable widespread field use and the need for accurate, sensitive assays to detect asymptomatic Leishmania infection has become apparent. The domestic dog serves as the primary reservoir host of L. infantum. Study of this reservoir population provides an opportunity to evaluate the sensitivity and specificity of diagnostics for well-defined, symptomatic, canine visceral leishmaniasis (CVL) and asymptomatic L. infantum infection. Blood samples from an L. infantum-endemic population of US hunting dogs were evaluated with Dual-Path Platform (DPP®) CVL compared to those obtained via direct detection methods (culture- and Leishmania-specific quantitative polymerase chain reaction, qPCR) and immunofluorescence anti-Leishmania antibody test (IFAT). Statistically significant correlations were found between DPP® CVL development time and clinical status, culture status, circulating DNA levels, and IFAT titer. DPP® CVL results correlated with both clinical severity of disease and serological evidence of asymptomatic L. infantum infection. By precisely documenting the minimum time required for the development of a clear positive result in DPP® CVL, this test could be used in a rapid, semi-quantitative manner for the evaluation of asymptomatic and symptomatic CVL. Our results also indicate that a similar test could be used to improve our understanding of human VL.

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“…Bunun dışında moleküler yöntemler veya parazit antijen saptanması yöntemleri de kullanılmaktadır. Yapılan bir çalışmada KL tanısı alan olguların %78.4'ünde direkt mikroskopi, %92'sinde kültür ve %81.1'inde PCR pozitifliği olduğu izlenmiş; KL tanısında kültür yönteminin en duyarlı yöntem olduğu saptanmış ve KL tanısında birden fazla yöntemin kullanılmasının duyarlılık ve özgüllüğü artıracağı ve daha çok sayıda olgunun tanımlanmasına olanak sağlayacağı bildirilmiştir (5) .…”

Section: öZetunclassified

“…Erime eğrisi analizi ile referans suşların erime eğrilerine göre incelenen suşların genotiplendirilmesi gerçekleştirilmiştir (8) . Lezyondan alınan seröz aspirattan yapılan yaymada mikroskobide Leishmania amastigotlarının görülmesi, NNN besiyerinde üremiş promastigotların görülmesi KL tanısının doğrulanmasında altın standart olup en çok tercih edilenidir (5) . Bizim hastamızda mikroskobi, kültür ve Real-Time PCR ile tanı konmuş ve etken L. tropica olarak saptanmıştır.…”

Section: Genotiplendirmeunclassified

Three Autochthonous Cutaneous Leishmaniasis Cases in Manisa

Özbilgin¹,

Yıldırım²,

Çavuş³

et al. 2016

TMCD

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Comparison of Direct Microscopy, Culture and Polymerase Chain Reaction Methods for the Diagnosis of Cutaneous Leishmaniasis

Cited by 11 publications

References 10 publications

Test accuracy of polymerase chain reaction methods against conventional diagnostic techniques for Cutaneous Leishmaniasis (CL) in patients with clinical or epidemiological suspicion of CL: Systematic review and meta-analysis

Test accuracy of polymerase chain reaction methods against conventional diagnostic techniques for Cutaneous Leishmaniasis (CL) in patients with clinical or epidemiological suspicion of CL: Systematic review and meta-analysis

Semi-quantitative measurement of asymptomatic L. infantum infection and symptomatic visceral leishmaniasis in dogs using Dual-Path Platform® CVL

Three Autochthonous Cutaneous Leishmaniasis Cases in Manisa

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