Comparison of DNA Breaks at Entrance Channel and Bragg Peak Induced by Fast C6+ Ions

Usami, Noriko; Kobayashi, Kazuo; Hirayama, Ryoichi; Furusawa, Yoshiya; Porcel, Erika; Lacombe, S.; Sech, C. Le

doi:10.1269/jrr.09035

Cited by 14 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…International Journal of Nanomedicine 2021:16 362 pH 8.4 and 1 mM of EDTA) at 25 V for 5 h at room temperature. [21][22][23][24][25][26][27][28][29][30][31][32][33] The gels were stained with ethidium bromide (1 μg/mL) for over 20 h and washed with pure water for 1 h. The gel images were captured using a highsensitive imager (ImageQuant LAS 4010, GE Healthcare, Chicago IL, US) under UV illumination. The fluorescence intensities of the three DNA bands were measured using an image analysis software (ImageQuant TL, GE Healthcare, Chicago IL, US).…”

Section: Dovepressmentioning

confidence: 99%

Effect of Gold Nanoparticle Radiosensitization on Plasmid DNA Damage Induced by High-Dose-Rate Brachytherapy

Yogo¹,

Misawa²,

Shimizu³

et al. 2021

IJN

Self Cite

View full text Add to dashboard Cite

Gold nanoparticles (AuNPs) are candidate radiosensitizers for medium-energy photon treatment, such as γ-ray radiation in high-dose-rate (HDR) brachytherapy. However, high AuNP concentrations are required for sufficient dose enhancement for clinical applications. Here, we investigated the effect of positively (+) charged AuNP radiosensitization of plasmid DNA damage induced by 192Ir γ-rays, and compared it with that of negatively (−) charged AuNPs. Methods: We observed DNA breaks and reactive oxygen species (ROS) generation in the presence of AuNPs at low concentrations. pBR322 plasmid DNA exposed to 64 ng/mL AuNPs was irradiated with 192Ir γ-rays via HDR brachytherapy. DNA breaks were detected by observing the changes in the form of the plasmid and quantified by agarose gel electrophoresis. The ROS generated by the AuNPs were measured with the fluorescent probe sensitive to ROS. The effects of positively (+) and negatively (−) charged AuNPs were compared to study the effect of surface charge on dose enhancement. Results: +AuNPs at lower concentrations promoted a comparable level of radiosensitization by producing both single-stranded breaks (SSBs) and double-stranded breaks (DSBs) than those used in cell assays and Monte Carlo simulation experiments. The dose enhancement factor (DEF) for +AuNPs was 1.3 ± 0.2 for SSBs and 1.5 ± 0.4 for DSBs. The ability of +AuNPs to augment plasmid DNA damage is due to enhanced ROS generation. While −AuNPs generated similar ROS levels, they did not cause significant DNA damage. Thus, dose enhancement using low concentrations of +AuNPs presumably occurred via DNA binding or increasing local +AuNP concentration around the DNA. Conclusion: +AuNPs at low concentrations displayed stronger radiosensitization compared to −AuNPs. Combining +AuNPs with 192Ir γ-rays in HDR brachytherapy is a candidate method for improving clinical outcomes. Future development of cancer cell-specific +AuNPs would allow their wider application for HDR brachytherapy.

show abstract

Section: Dovepressmentioning

confidence: 99%

Effect of Gold Nanoparticle Radiosensitization on Plasmid DNA Damage Induced by High-Dose-Rate Brachytherapy

Yogo¹,

Misawa²,

Shimizu³

et al. 2021

IJN

Self Cite

View full text Add to dashboard Cite

show abstract

“…When the LET of incident ions increases, the radical production increases as well, which may lead to a reduced number of radicals available to interact with the cell due to recombination of these radicals with each other (Usami et al. 2010; Terato et al. 2008).…”

Section: Conventional Radiotherapymentioning

confidence: 99%

Gold nanoparticles for cancer radiotherapy: a review

et al. 2016

View full text Add to dashboard Cite

Radiotherapy is currently used in around 50% of cancer treatments and relies on the deposition of energy directly into tumour tissue. Although it is generally effective, some of the deposited energy can adversely affect healthy tissue outside the tumour volume, especially in the case of photon radiation (gamma and X-rays). Improved radiotherapy outcomes can be achieved by employing ion beams due to the characteristic energy deposition curve which culminates in a localised, high radiation dose (in form of a Bragg peak). In addition to ion radiotherapy, novel sensitisers, such as nanoparticles, have shown to locally increase the damaging effect of both photon and ion radiation, when both are applied to the tumour area. Amongst the available nanoparticle systems, gold nanoparticles have become particularly popular due to several advantages: biocompatibility, well-established methods for synthesis in a wide range of sizes, and the possibility of coating of their surface with a large number of different molecules to provide partial control of, for example, surface charge or interaction with serum proteins. This gives a full range of options for design parameter combinations, in which the optimal choice is not always clear, partially due to a lack of understanding of many processes that take place upon irradiation of such complicated systems. In this review, we summarise the mechanisms of action of radiation therapy with photons and ions in the presence and absence of nanoparticles, as well as the influence of some of the core and coating design parameters of nanoparticles on their radiosensitisation capabilities.

show abstract

“…The radiosensitization of platinum-based nanoparticles has been explored (Porcel et al 2010; Usami et al 2005, 2007, 2008, 2010), especially in combination with fast atomic ions such as Fe 26+ and C 6+ . Unlike photons and electrons, such heavy atoms with large cross sections interact with tissues on their trajectory tracks, and therefore are more efficient than conventional radiations (Dan et al 2003; Mozumder 2003).…”

Section: Nanoparticles For Radiosensitizationmentioning

confidence: 99%

Synthetic nanoparticles for delivery of radioisotopes and radiosensitizers in cancer therapy

Zhao

Zhou

2016

Cancer Nano

View full text Add to dashboard Cite

Radiotherapy has been, and will continue to be, a critical modality to treat cancer. Since the discovery of radiation-induced cytotoxicity in the late 19th century, both external and internal radiation sources have provided tremendous benefits to extend the life of cancer patients. Despite the dramatic improvement of radiation techniques, however, one challenge persists to limit the anti-tumor efficacy of radiotherapy, which is to maximize the deposited dose in tumor while sparing the rest of the healthy vital organs. Nanomedicine has stepped into the spotlight of cancer diagnosis and therapy during the past decades. Nanoparticles can potentiate radiotherapy by specifically delivering radionuclides or radiosensitizers into tumors, therefore enhancing the efficacy while alleviating the toxicity of radiotherapy. This paper reviews recent advances in synthetic nanoparticles for radiotherapy and radiosensitization, with a focus on the enhancement of in vivo anti-tumor activities. We also provide a brief discussion on radiation-associated toxicities as this is an area that, up to date, has been largely missing in the literature and should be closely examined in future studies involving nanoparticle-mediated radiosensitization.

show abstract

Comparison of DNA Breaks at Entrance Channel and Bragg Peak Induced by Fast C6+ Ions

Cited by 14 publications

References 19 publications

Effect of Gold Nanoparticle Radiosensitization on Plasmid DNA Damage Induced by High-Dose-Rate Brachytherapy

Effect of Gold Nanoparticle Radiosensitization on Plasmid DNA Damage Induced by High-Dose-Rate Brachytherapy

Gold nanoparticles for cancer radiotherapy: a review

Synthetic nanoparticles for delivery of radioisotopes and radiosensitizers in cancer therapy

Contact Info

Product

Resources

About