Comparison of DNA Isolation Methods that Derived from Leaves of a Potential Anti-Cancer Rodent Tuber (Typhonium flagelliforme) Plant

Abstract

The content of polysaccharides, polyphenols, proteins, and RNA compounds is the main problem often found in Plants DNA isolation, which inhibit the process of DNA isolation. Comparing the methods of plant DNA isolation is necessary for obtaining the DNA with good quality, purity, high concentration and efficiency time and cost. This study aimed to determine the best DNA isolation method that derived from leaves of a potential anti-cancer Rodent Tuber (Typhonium flagelliforme) plant by comparing the conventional DNA isolation method (cetyl trimethyl ammonium bromide/CTAB) and 2 commercial kits (Promega Wizard™ Genomic DNA Purification Kits, and Geneaid Genomic Mini Kit). The results showed that the CTAB method yielded a higher amount of DNA (>100 ng/µL) at the cost of 0.49 USD per sample, in comparison with Promega method (69.19 to 157.68 ng/µL) at 3.28 USD per sample and Geneaid method (8.15 to 18.52 ng/µL) at 2.06 USD per sample. Based on the purity of isolated DNA (A260/280), CTAB method produced relatively similar DNA quality to Promega kit (1.8-2.0). On the other hand, Geneaid method resulted in a lower purity value at 1.15 to 1.60.

Evaluation of three different DNA extraction methods for the detection of Pepper yellow leaf curl virus (PepYLCV) by Polymerase Chain Reaction

“…This suggestion is supported by several previous studies with the successful detection of PepYLCV, in chili and sweet potato plants in Indonesia [16,45,46]. Furthermore, the CTAB method produced high concentrations (>100 ng/µL) and purity (1.8-2.0) at a cost of $0.49 per sample [47].…”

Optimizing DNA Extraction and Selecting Suitable Regions for Biodiversity Assessment: A Study on Shorea leprosula

“…On the other hand, the GeneAid plant extraction kit utilizes a different approach based on silica-based membrane technology in the form of a spin column (Rahmadara et al, 2022). Although this method is convenient and can yield satisfactory results for many applications, it may not be as effective in removing certain contaminants and impurities as the CTAB method.…”

“…Gel electrophoresis revealed that the CTAB method yielded distinct bands of high-molecularweight DNA, including smeared bands of contaminants, in comparison to the DNA isolated by the GeneAid method (Figure 1). According to Rahmadara et al (2022), a clear band without smearing in the visualization results indicates good-quality DNA isolation, while the presence of smears suggests the presence of contaminants in the extraction results. Interestingly, no bands were observed when DNA was isolated from wood samples using either the CTAB extraction or the GeneAid Kit.…”

“…Interestingly, no bands were observed when DNA was isolated from wood samples using either the CTAB extraction or the GeneAid Kit. The GeneAid kit utilizes silica-based membrane technology in the form of a spin column (Dhaliwal, 2020); however, it appears to be less effective in removing polyphenolic and protein compounds (Rahmadara et al, 2022).…”