Comparison of DNA probe test and cultivation methods for detection of Mycobacterium avium subsp paratuberculosis in caprine and ovine faeces

Dimareli-Malli, Z.; Sarris, K.

doi:10.1111/j.1751-0813.2001.tb10640.x

Cited by 8 publications

(6 citation statements)

References 5 publications

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“…Moreover, the overall sensitivities of cultures in this study were 72.9 and 37.6% in cattle and goats, respectively, and are in agreement with data from previous studies (14,40,48). This difference in the isolation may indicate the difficulty of the isolation of a certain group of strains because of several factors that must be studied, such as very limited bacterial burden in the sample, conditions of preservation, increased sensitivity to the decontamination protocol, specific nutritional requirements, and increased sensitivity to the inhibitory effect of antibiotics in the media.…”

Section: Discussionsupporting

confidence: 82%

“…paratuberculosis strains by culture are directly related to the type of M. avium subsp. paratuberculosis strain and not to the host (animal species), as has previously been accepted (8,14,36). This fact has to be taken into account when a protocol for the isolation of M. avium subsp.…”

Section: Discussionmentioning

confidence: 99%

“…Since the first in vitro isolation of M. avium subsp. paratuberculosis (44), the culture protocol and media have been improved, although there are still two major problems: (i) the long incubation periods required to obtain visible colonies (5,45), especially when working with animals infected by extremely slow growing isolates (12,14,36), and (ii) the failure to isolate M. avium subsp. paratuberculosis isolates in some samples, depending on the host (23), the clinical sample (tissue or feces) (41,46,48,51), and the disease stage (clinical or subclinical) (19,25).…”

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confidence: 99%

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Comparison of Four Different Culture Media for Isolation and Growth of Type II and Type I/III Mycobacterium avium subsp. paratuberculosis Strains Isolated from Cattle and Goats

Juan

Álvarez

Romero

et al. 2006

Appl Environ Microbiol

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has different requirements for growth in the culture media studied. The recommended solid media for isolation of type I/III strains are LJm and Mm, since the combination of both media allowed the recovery of all these strains. The most widespread culture medium, HEYM, is not suitable for the isolation of this group of M. avium subsp. paratuberculosis strains. Regarding the type II strains, HEYMm-SP was the medium where more strains were isolated, but the other three media are also needed in order to recover all type II strains. The incubation period is also related to the strain type. In conclusion, because the type of strain cannot be known in advance of culture, coupled with the fact that cattle and goats can be infected with both groups of strains, we recommend the use of the four solid media and the prolongation of the incubation period to more than 6 months to detect paratuberculous herds/flocks and to determine the true prevalence of the infection.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Comparison of Four Different Culture Media for Isolation and Growth of Type II and Type I/III Mycobacterium avium subsp. paratuberculosis Strains Isolated from Cattle and Goats

Juan

Álvarez

Romero

et al. 2006

Appl Environ Microbiol

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“…It is widespread in small ruminants in Greece (Xenos et al., 1984,Dimareli et al., 1991) and presents a serious problem causing significant economic losses. Goats are more susceptible to the disease than sheep (Xenos et al., 1984; Dimareli‐Malli and Sarris, 2001). The disease was not reported in cattle until 1988, despite their frequent contact with goats and sheep, when it appeared in at least 10 cattle herds during 1988–2003 (Dimareli‐Malli et al., 1998).…”

Section: Introductionmentioning

confidence: 99%

Study of Microbiological and Molecular Typing Aspects of Paratuberculosis in Sheep and Goats in Northern Greece

Dimareli-Malli

Stevenson

Sarris

et al. 2009

Transboundary and Emerging Diseases

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Mycobacterium avium subsp. paratuberculosis, an acid-fast bacterium is the agent of Johne's disease, an intestinal disease that causes poor nutrient intake in ruminants. During the period 1987-2003, 322 of 777 (41.4%) goat herds and 97 of 458 (21.1%) sheep flocks were found to be infected with M.a.paratuberculosis in Northern Greece. From goats, mycobacteria were isolated from 238 of 652 (36.5%) of intestinal tissues, 14 of 119 (11.8%) of lymph nodes and five of 369 (1.4%) of faecal samples. From sheep, mycobacteria were isolated from 25 of 162 (15.4%) of intestinal tissues, three of 41 (7.3%) of lymph nodes and two of 322 (0.6%) of faecal samples. Isolates were typed by pulsed-field gel electrophoresis (PFGE) and restriction fragment length polymorphism followed by hybridization to IS900 [IS900- restriction fragment-length polymorphism (RFLP)]. IS900-RFLP BstEII profiles C1 and C5 and PFGE profiles [2-19] and [29-15] were identified. These PFGE profiles have not been found outside Greece to date.

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“…Since then, the number of infected herds as well as the within-herd prevalence in infected herds grew rapidly, while the disease has spread to most parts of the country via movement of infected stock or flockto-flock transmission. Goats are more susceptible than sheep [16,17].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Live Paratuberculosis Vaccine in Endemically Infected Flocks of Sheep and Goats in Greece

Dimareli-Malli¹,

Papadopoulos²,

Betsas³

2013

JIBTVA

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A large 7-year vaccination trial was conducted in 15 flocks of goats and 7 flocks of sheep, known to be infected with Mycobacterium avium subsp. paratuberculosis (MAP), in Northern Greece. A total of 3665 kids and 1685 lambs, 7-30 days old, were vaccinated during 1995-1999. Seven hundred and seventy-five kids and 413 lambs were kept as unvaccinated controls. For each trial, the Incidence Rate Ratio (IRR), with respective exact 95% confidence intervals, was calculated. All IRR point estimates for young animals were very large (from 5.68 to 11.78 for kids and from 4.28 to 10.08 for lambs), while none of the 95% confidence intervals included 1. The protective effect of vaccination was large and the difference in mortality among vaccinated and unvaccinated animals was more pronounced in young animals. The effect in adult animals was smaller than in young animals; it was, however, still considerable. Upon visual inspection of the K-M curves, it seems that for the young animal trials the vaccinated and control-group curves were diverging increasingly over time, which indicates that the gain from the vaccination (or the loss from non-vaccination) might increase over time during the trial.

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Comparison of DNA probe test and cultivation methods for detection of Mycobacterium avium subsp paratuberculosis in caprine and ovine faeces

Abstract: The DNA probe test is a rapid and specific test that could be used in a control program if the sensitivity of the test were improved and possibly in combination with another test.

Cited by 8 publications

References 5 publications

Comparison of Four Different Culture Media for Isolation and Growth of Type II and Type I/III Mycobacterium avium subsp. paratuberculosis Strains Isolated from Cattle and Goats

Comparison of Four Different Culture Media for Isolation and Growth of Type II and Type I/III Mycobacterium avium subsp. paratuberculosis Strains Isolated from Cattle and Goats

Study of Microbiological and Molecular Typing Aspects of Paratuberculosis in Sheep and Goats in Northern Greece

Evaluation of a Live Paratuberculosis Vaccine in Endemically Infected Flocks of Sheep and Goats in Greece

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