Comparison of Effects of Pulsed Electromagnetic Field Stimulation on Platelet-Rich Plasma and Bone Marrow Stromal Stem Cell Using Rat Zygomatic Bone Defect Model

Kapi, Emin; Bozkurt, Mehmet; Selçuk, Caferi Tayyar; Celik, Mehmet Salih; Akpolat, Veysi; Isik, Fatma Birgul; Bozarslan, Beri Hocaoglu; Çelik, Yusuf

doi:10.1097/sap.0000000000000160

Cited by 11 publications

(12 citation statements)

References 57 publications

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“…Osteopontin scoring was higher in the bone group, which correlates with the study by Kapi et al 34 Other histological parameters were similar and correlated with 2 previous studies, 11,31 but no evaluation of electron microscopy was performed in either study. According to the SEM results, Ca/P ratios were similar, and there was no significant difference between the ratios stated in the literature.…”

Section: Discussionsupporting

confidence: 90%

Histological and Immunological Evaluation of the Osteogenic Effects of Compact Bone–Delivered Stem Cell on Spongiosis Bone in the Rat Zygomatic Arch Defect Model

et al. 2023

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Background In stem cell applications, apart from bone marrow and adipose tissue, compact bone is also used as an alternative. However, studies on this subject are limited. In our study, we investigated the effect of stem cell derived from compact bone on rat zygomatic arch defect. Methods Fifteen rats were included in the study. Five rats were killed to obtain stem cells before the experiment. The rats were divided into 2 groups with 5 rats each. In group 1, compact bone–derived stem cell was applied. In group 2, adipose tissue–derived stem cell was applied. Right zygomatic arch defect was created in rats in both groups. Zygomatic bones were decellularized by cryosurgery. Stem cells were transferred to zygomatic bones. The number of stem cells, stem cell differentiation, and superficial markers obtained from the groups were examined. Histologically, cell structure, osteocyte count and osteopontin scores, elemental composition of the groups, percentages of resemblance to intact bone, osteocytes numbers, and cells were examined by electron microscopy of the bones in the groups after killing. Results The number of stem cells administered to the groups was 5 × 107 and 3.2 × 107 for group 1 and group 2, respectively (P > 0.05). Histologically, the morphology of the cells in group 1 was found to be healthier than group 2. The number of osteocytes was 97.56 ± 15.4 and 132.93 ± 10.8 in group 1 and group 2, respectively (P < 0.05). The osteopontin score was 3.47 ± 0.73 and 65 ± 0.64 in group 1 and group 2, respectively (P < 0.05). In the electron microscope examination, the morphologies of the cells in group 1 were seen more normal. The Ca/P ratio of the groups was 1.51 and 1.59 in group 1 and group 2, respectively (P > 0.05). Osteocyte counts were 10.7 ± 2.8 and 6.1 ± 1.2 in group 1 and group 2, respectively (P < 0.05). Morphological similarity percentages to normal bone were 88.4% and 79.6% in group 1 and group 2, respectively (P > 0.05). Conclusion Stem cells obtained from compact bone gave positive results in zygomatic arch defect. This method can also be used as an alternative in stem cell applications.

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Section: Discussionsupporting

confidence: 90%

Histological and Immunological Evaluation of the Osteogenic Effects of Compact Bone–Delivered Stem Cell on Spongiosis Bone in the Rat Zygomatic Arch Defect Model

et al. 2023

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“…Moreover, an animal study demonstrated that PEMFs could enhance angiogenesis in both normal mice and diabetic mice [ 53 ]. Interestingly, the effect of PEMFs on platelet rich plasma mediates a beneficial and effective combination in terms of bone regeneration [ 54 ]. In fibroblast-like cells derived from mononuclear peripheral blood cells, PEMF irradiation protocol decreased some of the most important proinflammatory cytokine secretion such as TNF- α and IL-1 β and a significant increase in cytokines of lymphocytic origin such as IL-10 [ 55 ].…”

Section: Effect Of Low Frequency Low Energy Pulsed Electromagneticmentioning

confidence: 99%

Adenosine Receptors as a Biological Pathway for the Anti-Inflammatory and Beneficial Effects of Low Frequency Low Energy Pulsed Electromagnetic Fields

Varani

Vincenzi

Ravani

et al. 2017

Mediators of Inflammation

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Several studies explored the biological effects of low frequency low energy pulsed electromagnetic fields (PEMFs) on human body reporting different functional changes. Much research activity has focused on the mechanisms of interaction between PEMFs and membrane receptors such as the involvement of adenosine receptors (ARs). In particular, PEMF exposure mediates a significant upregulation of A2A and A3ARs expressed in various cells or tissues involving a reduction in most of the proinflammatory cytokines. Of particular interest is the observation that PEMFs, acting as modulators of adenosine, are able to increase the functionality of the endogenous agonist. By reviewing the scientific literature on joint cells, a double role for PEMFs could be hypothesized in vitro by stimulating cell proliferation, colonization of the scaffold, and production of tissue matrix. Another effect could be obtained in vivo after surgical implantation of the construct by favoring the anabolic activities of the implanted cells and surrounding tissues and protecting the construct from the catabolic effects of the inflammatory status. Moreover, a protective involvement of PEMFs on hypoxia damage in neuron-like cells and an anti-inflammatory effect in microglial cells have suggested the hypothesis of a positive impact of this noninvasive biophysical stimulus.

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“…Conventional methods include conservative exercise and orthosis intervention. Although there is certain effect, most patients are still difficult to recover in time after conventional treatment [7] . PRP is gained through autoblood sample treatment.…”

Section: Discussionmentioning

confidence: 99%

“…After the completion of treatment, conventional treatment was done for the patient's wound, and the orthosis was used to fix. In a short time, walking with load would not be implemented [4] . After 3M, the treatment conditions of both groups were reexamined.…”

Section: Methodsmentioning

confidence: 99%

Evaluation on Clinical Effect of Platelet Rich Plasma in Treatment of Non-infective Bone Ununion

Zhang¹,

Cheng²

2018

2018 7th International Conference on Medical Engineering and Biotechnology (MEDEB 2018)

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Objective: to explore curative effect of platelet rich plasma on non-infective bone ununion. Method: this research was conducted from January 2016 to October 2017. 80 patients with non-infective bone ununion in the research period were chosen as the object of research, and they were classified into control group (40 cases, conventional therapy) and observation group (40 cases, conventional therapy + platelet rich plasma treatment) with random number method. After the treatment, the effect was observed and inter-group evaluation and comparison were conducted. Results: bone fracture healing time of patients in the observation group was (5.46±1.36)M, shorter than that of control group ((8.16±1.21)M). Besides, bone fracture healing rate of patients in the observation group after 3M was 82.50%, significantly higher than the control group (45.00%). The inter-group difference was significant (P<0.05). Conclusion: platelet rich plasma has good clinical effect on treatment of non-infective bone ununion, so it deserves to be promoted and applied in similar diseases.

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Comparison of Effects of Pulsed Electromagnetic Field Stimulation on Platelet-Rich Plasma and Bone Marrow Stromal Stem Cell Using Rat Zygomatic Bone Defect Model

Cited by 11 publications

References 57 publications

Histological and Immunological Evaluation of the Osteogenic Effects of Compact Bone–Delivered Stem Cell on Spongiosis Bone in the Rat Zygomatic Arch Defect Model

Histological and Immunological Evaluation of the Osteogenic Effects of Compact Bone–Delivered Stem Cell on Spongiosis Bone in the Rat Zygomatic Arch Defect Model

Adenosine Receptors as a Biological Pathway for the Anti-Inflammatory and Beneficial Effects of Low Frequency Low Energy Pulsed Electromagnetic Fields

Evaluation on Clinical Effect of Platelet Rich Plasma in Treatment of Non-infective Bone Ununion

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