Comparison of electrical properties of M- and λ-DNA attached on the Au metal electrodes with nanogap

Lee, J.M.; Ahn, S.K.; Kim, K.S.; Lee, Y.; Roh, Yonghan

doi:10.1016/j.tsf.2005.12.185

Cited by 6 publications

(6 citation statements)

References 15 publications

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“…In an attempt to enhance the conductivity of DNA, Rakitin et al (18) substituted imino proton of each basepair with a metallic ion. For the same purpose, several authors also studied the conductivity through modified DNA (2,(19)(20)(21)(22)(23). In particular, an interesting approach was developed by Maruccio et al (20), who proposed a prototype transistor based on a deoxyguanosine derivative.…”

Section: Introductionmentioning

confidence: 99%

Humidity Dependence of Charge Transport through DNA Revealed by Silicon-Based Nanotweezers Manipulation

Yamahata

Collard

Takekawa

et al. 2008

Biophysical Journal

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The study of the electrical properties of DNA has aroused increasing interest since the last decade. So far, controversial arguments have been put forward to explain the electrical charge transport through DNA. Our experiments on DNA bundles manipulated with silicon-based actuated tweezers demonstrate undoubtedly that humidity is the main factor affecting the electrical conduction in DNA. We explain the quasi-Ohmic behavior of DNA and the exponential dependence of its conductivity with relative humidity from the adsorption of water on the DNA backbone. We propose a quantitative model that is consistent with previous studies on DNA and other materials, like porous silicon, subjected to different humidity conditions.

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Section: Introductionmentioning

confidence: 99%

Humidity Dependence of Charge Transport through DNA Revealed by Silicon-Based Nanotweezers Manipulation

Yamahata

Collard

Takekawa

et al. 2008

Biophysical Journal

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“…The exponent of 1.05 is shifted from 1.0, indicating the complicated conductive process from the collapsed ANP, as discussed above. In other words, the calibrating equation is dependent on the employed approach to bridge the gapped electrodes. − The “control” experiments from one-base-mismatch and from two-base-mismatch are also listed, suggesting the needed specificity of this detection approach. As for the detection limit of 5 × 10 −14 M, since ∼5.0 mL of sample solution was used for hybridization and the hybridization efficiency was less than 30%, − each gap array could catch a maximum of 5 × 10 5 target molecules (one chip consisted of 100 arrays).…”

Section: Resultsmentioning

confidence: 99%

“…There is a need for multistep enhancement and all the washing, drying, and measurement cycles in between. An alternative version is to shorten the gap distance, from ∼20 μm to less than 150 nm, , to increase the responding current signal. The signal from the background or control, however, is also increased correspondingly.…”

mentioning

confidence: 99%

Electrical Detection of Oligonucleotide Using an Aggregate of Gold Nanoparticles as a Conductive Tag

et al. 2008

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Sequence-specific DNA detection is a routine job in medical diagnostics and genetic screening. Alternative to a fluorescence readout scheme or electrophoresis approach, various kinds of rapid, low-cost, facile, and label-free methods have also been developed in last decades. Among these, direct electrical detection of DNA received increasing attention but more research is desirable. Particularly, enhancement with high discrimination must be employed to selectively amplify the responding signal. A chip-based biosensor was developed in this work to electrically detect 22-mer oligonucleotide DNA at low concentration, from 50 fM to 10 pM. First, a gold nanoparticle (NP) was capped with 3-mercaptopropionic acid through a thiol-gold bond. The derivatized carboxylic acid group showed strong complex interaction with an inorganic linker, Zr(4+). As a result, Zr(4+) could link several hundreds of individual gold NPs together to form an aggregate of nanoparticles (ANP), which was capable of being used as a conductive tag for the electrical detection of DNA. Second, in order to achieve the discriminative localization of ANP to bridge two comb-shaped electrodes (with height of approximately 50 nm and interdistance of 300-350 nm) gapped with insulative material of silicon oxide, peptide nucleic acids were covalently bonded to the silicon oxide in the gap as capture sites for DNA. After hybridization with target DNA, the charged phosphate-containing backbone of DNA was introduced into the gap. Phosphate groups also exhibited strong complex interaction with the linker of Zr(4+) and could react with the residual Zr(4+) on the ANP surface. As a consequence, the conductive tags were linked to the phosphate groups and localized into the gap, which could modify the conductance between the two comb-shaped electrodes in turn. The degree of modification correlated directly to the amount of hybridized DNA and to the concentration of target DNA in sample solution. Compared with the individual NPs used as the tag, a strong enhancement from the gold ANP was obtained.

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“…Lee et al reported on the metallic property of a modified M-DNA by inserting divalent zinc ions (Zn 2+ ) in every base pair of λ-DNA molecules [44]. M-DNA [45], the abbreviation of metal-containing DNA, is a complex between the divalent metal ions (Zn 2+ , Ni 2+ or Co 2+ ) and double helix DNA.…”

Section: Conductormentioning

confidence: 99%

Itinerant electron model and conductance of DNA

et al. 2008

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DNA (Deoxyribonucleic acid) has recently caught the attention of chemists and physicists. A major reason for this interest is DNA's potential use in nanoelectronic devices, both as a template for assembling nanocircuits and as an element of such circuits. However, the electronic properties of the DNA molecule remain very controversial. Charge-transfer reactions and conductivity measurements show a large variety of possible electronic behavior, ranging from Anderson and bandgap insulators to effective molecular wires and induced superconductors. In this review article, we summarize the wide-ranging experimental and theoretical results of charge transport in DNA. An itinerant electron model is suggested and the effect of the density of itinerant electrons on the conductivity of DNA is studied. Calculations show that a DNA molecule may show conductivity from insulating to metallic, which explains the controversial and profuse electric characteristics of DNA to some extent.

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Comparison of electrical properties of M- and λ-DNA attached on the Au metal electrodes with nanogap

Cited by 6 publications

References 15 publications

Humidity Dependence of Charge Transport through DNA Revealed by Silicon-Based Nanotweezers Manipulation

Humidity Dependence of Charge Transport through DNA Revealed by Silicon-Based Nanotweezers Manipulation

Electrical Detection of Oligonucleotide Using an Aggregate of Gold Nanoparticles as a Conductive Tag

Itinerant electron model and conductance of DNA

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