Comparison of Electrochemical Immunosensors and Aptasensors for Detection of Small Organic Molecules in Environment, Food Safety, Clinical and Public Security

Piro, Benoı̂t; Shi, Shihui; Reisberg, S.; Noël, Vincent; Anquetin, Guillaume

doi:10.3390/bios6010007

Cited by 47 publications

(26 citation statements)

References 90 publications

(95 reference statements)

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“…Electrochemical Impedance Spectroscopy (EIS) has been extensively employed in the characterization of the electrical properties of SPEs combined with nanomaterials, which are used as sensors for voltammetric, amperometric or potentiometric detections . The majority of SPE sensors that use EIS as a detection method are those based on the immobilization of a biological recognition element on the surface, such as enzymatic sensors, aptasensors and immunosensors . In these cases, the impedimetric detection consists of an increase of the charge transfer resistance (R CT ) due to the strong and specific binding of the analyte to the surface.…”

Section: Introductionmentioning

confidence: 99%

Nickel Hydroxide Nanoparticles on Screen‐printed Electrodes as an Impedimetric Non‐enzymatic Glucose Sensor

2017

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In this study, the electrodeposition of nickel hydroxide nanoparticles onto a screen‐printed electrode (Ni(OH)2/SPE) is described. Ni(OH)2/SPE is proposed as an alternative non‐enzymatic glucose sensor based on Electrochemical Impedance Spectroscopy (EIS) measurements.The SPEs were modified by the cathodic electrodeposition of nickel, from a solution containing 0.010 M Ni(NO3)2 and 1 M NH4Cl, at −1.3 V for 60 seconds. The SEM images show a uniform distribution of nickel spherical nanoparticles, with 60 nm average particle size. However, such morphology is not observed when the electrodeposition occurs in the absence of NH4Cl. The electrochemical properties of the sensor were carefully evaluated by Cyclic Voltammetry. Ni(OH)2/SPE shows a remarkable electrocatalytic behavior towards the oxidation of glucose in 0.1 M KOH. EIS measurements were carried out for Ni(OH)2/SPE and a single‐frequency impedance method is proposed as transduction principle for glucose determination. The analysis of each parameter of complex impedance was performed. The best linear response was obtained for the module of impedance (|Z|) in the range of 0–2 mM of glucose at 0.1 Hz (R2=0.992) with a slope of 0.137 KΩ−1⋅mM−1 of glucose. Finally, Ni(OH)2/SPE was utilized for quantification of glucose in blood samples.

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Section: Introductionmentioning

confidence: 99%

Nickel Hydroxide Nanoparticles on Screen‐printed Electrodes as an Impedimetric Non‐enzymatic Glucose Sensor

2017

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“…Aptasensors are biosensors in which the biological recognition elements are DNA or RNA aptamers. 159 In recent years, more and more aptasensors have been designed for possible diagnostic applications. Major detection methods include (a) optical transduction, (b) electrochemical detection and (c) mass sensitive detection.…”

Section: Aptasensor Methodsmentioning

confidence: 99%

Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

Pan

Liu

Zhang

2018

Journal of Infection

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The entire human population is at risk of infectious diseases worldwide. Thus far, the diagnosis and treatment of human infectious diseases at the molecular and nanoscale levels have been extremely challenging tasks because of the lack of effective probes to identify and recognize biomarkers of pathogens. Oligonucleotide aptamers are a class of small nucleic acid ligands that are composed of single-stranded DNA (ssDNA) or RNA and act as affinity probes or molecular recognition elements for a variety of targets. These aptamers have an exciting potential for diagnose and/or treatment of specific diseases. In this review, we highlight areas where aptamers have been developed as diagnostic and therapeutic agents for both bacterial and viral infectious diseases as well as aptamer-based detection.

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“…Biosensors using an aptamer as a recognition element are called "aptasensors". According to Piro et al (2016), the sensitivity of immunosensors seems to be often better than the sensitivity of aptasensors [69]. However, recent progress in transduction and signal amplification has led to the development of many sensitive aptasensors.…”

Section: Aptasensors For the Detection Of Antimicrobial Residuesmentioning

confidence: 99%

The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products

Gaudin¹

2020

Biosensors

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The presence of antimicrobial residues in food-producing animals can lead to harmful effects on the consumer (e.g., allergies, antimicrobial resistance, toxicological effects) and cause issues in food transformation (i.e., cheese, yogurts production). Therefore, to control antimicrobial residues in food products of animal origin, screening methods are of utmost importance. Microbiological and immunological methods (e.g., ELISA, dipsticks) are conventional screening methods. Biosensors are an innovative solution for the development of more performant screening methods. Among the different kinds of biosensing elements (e.g., antibodies, aptamers, molecularly imprinted polymers (MIP), enzymes), aptamers for targeting antimicrobial residues are in continuous development since 2000. Therefore, this review has highlighted recent advances in the development of aptasensors, which present multiple advantages over immunosensors. Most of the aptasensors described in the literature for the detection of antimicrobial residues in animal-derived food products are either optical or electrochemical sensors. In this review, I have focused on optical aptasensors and showed how nanotechnologies (nanomaterials, micro/nanofluidics, and signal amplification techniques) largely contribute to the improvement of their performance (sensitivity, specificity, miniaturization, portability). Finally, I have explored different techniques to develop multiplex screening methods. Multiplex screening methods are necessary for the wide spectrum detection of antimicrobials authorized for animal treatment (i.e., having maximum residue limits).

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Comparison of Electrochemical Immunosensors and Aptasensors for Detection of Small Organic Molecules in Environment, Food Safety, Clinical and Public Security

Cited by 47 publications

References 90 publications

Nickel Hydroxide Nanoparticles on Screen‐printed Electrodes as an Impedimetric Non‐enzymatic Glucose Sensor

Nickel Hydroxide Nanoparticles on Screen‐printed Electrodes as an Impedimetric Non‐enzymatic Glucose Sensor

Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products

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