Comparison of Elisa and Rapid Immunochromatographic Tests in Diagnosis of Toxoplasmosis in Port Harcourt, Nigeria

Onosakponome, E. O.; Wokem, G. N.; Abah, A. E.

doi:10.9734/ijtdh/2020/v41i130247

Cited by 12 publications

(8 citation statements)

References 9 publications

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“…7 And also higher than those reported from healthy-individual from different part of Nigeria which ranged from 20.83% -41.13%. [8][9][10] The overall prevalence of 2.6% HIV recorded was lesser than that of 8.4% reported by Eaton in 2014, 67% recorded in Ethiopia 11 but higher than that of 0.36% in Benin city, 0.28% in Bendel State 12 and 1.58% in Lesotho. 13 The co-infection of 1.5% in the study is lesser than that of 25.1% in Asia, 49.1% in Latin and Caribbean and 60.7% in North America and Middle East.…”

Section: Discussionmentioning

confidence: 76%

Prevalence and co-infection of Toxoplasma gondii and Human Immunodeficiency Virus (HIV) infection among women of child-bearing age in Osun state Nigeria

Olaniran¹,

OO²,

NP³

et al. 2022

ICPJL

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This study assessed the epidemiology of Toxoplasma gondii and Human immunodeficiency Virus and their co-infection infection in women of child-bearing age in communities in Osun State, Nigeria. The study also determines the factors that facilitate the organisms among the studied groups and possible interaction between the prevalence of infection and the risk factors. These were done with a view to providing baseline information on the mode of T. gondii and HIV transmission. Sera were analyzed for the presence of IgG and IgM antibodies against T. gondii by commercially available enzyme linked immunosorbent assay (ELISA) kit (Demeditec Diagnostics GmbH, Germany) conducted according to the manufacturer’s instructions. The optical densities of wells were measured by a photometer at a wavelength of 450 nm and the detection of HIV was done using (Determine) rapid immunochromatographic (IC) test which are in strip form. Determination of the blood groups of the donors was done using commercially prepared Anti sera A, B, AB and anti D that determine the Rhesus blood group. The overall prevalence of T. gondii among the women of child-bearing age in selected six communities in Osun state was 74.2%, while the overall prevalence of HIV among the women was 2.6% and co-infection of T. gondii and HIV was 1.5%. The prevalence of T. gondii was lowest (57.8%) among women from Ile Ife, a peri-urban community and highest (100%) in women residing in Alajue, a rural community. The prevalence of T. gondii infection was significantly higher (p= 0.001) among Islamic women (85.9%) than in Christian women (68.2%) while the prevalence of HIV (2.7%) in Christianity and co-infection of T. gondii and HIV (2.3%) was higher among Islam. The highest prevalence of T. gondii (83.6%) was recorded in women with primary education while the lowest of (58.7%) was recorded in women with tertiary education (p =0.037) while the highest prevalence of HIV infection of 2.9% and co-infection of 1.8% was recorded in secondary school level. The highest prevalence of T. gondii (78.5%) was recorded in women that reside in rural area and the lowest (67.5%) was recorded in women that reside in peri-urban area (p=0.016) and also the prevalence of HIV (4.5%) (p= 0.045) and co-infection of T. gondii and HIV (3.2%) (p=0.025) was higher in peri-urban area. The highest prevalence of 84.0% of T. gondii was recorded in house wives while the lowest value of 59.3% was recorded in civil servant and the highest prevalence of HIV infection of 7.7% in house wives and co-infection of 3.3% was recorded in trading. The highest prevalence of T. gondii infection of 100.0% was recorded in women with blood group AB negative while the lowest prevalence of 30.0% was recorded in women with blood group A negative and the highest prevalence of HIV infection of 6.3% was recorded in women with blood group B negative and O negative each. Co-infection has highest prevalence of 6.3% in blood group B negative. The highest prevalence of 77.1% of T. gondii was recorded in women with one miscarriage while the lowest value of 73.3% was recorded in women with two miscarriages and the highest prevalence of HIV infection of 5.7% was recorded in women with one miscarriage while the lowest prevalence of 2.5% was recorded in women with no miscarriage. The highest prevalence of 100.0% of T. gondii and 2.7% of HIV infection was recorded in women with no pregnancy and the co-infection 1.6% was recorded in women no pregnancy. The study concluded that there was high prevalence of T. gondii infection (74.2%) and also implicates HIV (2.6%) and co-infection of both (1.5%) in the studied population. Hence, there is the need for health education and create awareness of the diseases and its transmission to women of reproductive age group in general and pregnant women in particular to reduce the risk of T. gondii and HIV in pregnant women.

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Section: Discussionmentioning

confidence: 76%

Prevalence and co-infection of Toxoplasma gondii and Human Immunodeficiency Virus (HIV) infection among women of child-bearing age in Osun state Nigeria

Olaniran¹,

OO²,

NP³

et al. 2022

ICPJL

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“…The differences observed between the infection rates reported by the health facilities in our study and those obtained at the LIM of the CIRCB, using the same methods and on the same samples, illustrate the difficulties in interpreting serological results for toxoplasmosis [ 13 ]. In Batch1, the sensitivity of the RDT (12%) for IgG detection is much lower than the 54.4% obtained in Egypt [ 33 ], the 46.7% found in Nigeria [ 34 ], and the 96% obtained at LIM/CIRCB with the same technique. These differences could be related to the handling conditions on the one hand and the quality of the kits used on the other.…”

Section: Discussionmentioning

confidence: 82%

Performance of immunochromatographic and immunoenzymatic techniques in the diagnosis of toxoplasmosis in pregnant women in Cameroon: need for harmonization

Yéngué¹,

Ntsama²,

Ambada³

et al. 2022

Pan Afr Med J

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Introduction in order to contribute to the improvement of the management of toxoplasmosis in pregnant women in Cameroon, performance of two techniques commonly used in the diagnosis of toxoplasmosis was evaluated. Methods a total of 541 pregnant women were recruited from seven hospitals in two Regions of Cameroon, of which 63% (341: Batch1) were from health facilities (HF) using a immunochromatographic technique (ICT) as a screening test for toxoplasmosis, and 37% (200: Batch2) from those using an immunoenzymatic technique (IEZ). On each sample, Ig (Immunoglobulin) G (IgG) and IgM were tested by three techniques: a Rapid Diagnostic Test (RDT), an Enzyme Linked Immuno Sorbent Assay (ELISA) and a Vidas Enzyme-linked fluorescent assay taken as reference (VIDAS/ELFA). The results from the health facilities were recorded. Results for the IgG assay, our two laboratory methods were sensitive (96.0% and 97.5%) and specific (64.2% and 59.7%). Their concordance rates with the VIDAS/ELFA reference were above 60% (P<0.001). Moreover, for the IgM assay, the performances of the two methods were equivalent: Se= 18.2%, Sp= 99.4% with a low concordance rate (Kappa = 0.24). Considering the results provided by the selected hospitals, the ELISA used in Batch2 showed similar performances to the two techniques used in reference lab while the performances were low for the RDT used in Batch1. Conclusion both methods showed similar performances (good for (IgG) and poor for IgM). However, for the immunochromatographic method, differences in performance were found between our results and those provided by the selected health facilities. These differences suggest a harmonization of diagnostic techniques for toxoplasmosis in pregnant women in Cameroonian health facilities.

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“…Each individual had 2 ml of blood drawn from them via venipuncture into an uncomplicated tube under aseptic conditions. Sample was centrifuged to obtain serum which was used for laboratory analysis using enzyme linked immunosorbent assay (ELISA) for chlamydia IgG antibodies determination, catalogue number BC-1071 purchased from Bio check with MAP LAB plus microwell reader [15,16]. The sensitivity and specificity of IgG antibody test for Chlamydia trachomatis is 100% and 97.6% respectively, [17].…”

Section: Sample Collection and Preparationmentioning

confidence: 99%

The Sero-prevalence and Risk Factors of Chlamydiosis among Women of Reproductive Age in Port Harcourt, Nigeria

Ikpeama, R. A.,

Nyenke, C. U.,

Onosakponome, E. O.

et al. 2024

SAJRM

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Aim: This study is aimed to determine the association between chlamydiosis and some selected risk factors among women of reproductive age in Port Harcourt, Nigeria. Study Design: The study was a cross-sectional study conducted at Rivers State University teaching hospital. Place and Duration of the Study: The study was conducted at Rivers State University teaching hospital, Rivers State, Nigeria between March, 2022 and August, 2022. Methodology: This cross-sectional study included 450 women who ranged in age from 15 to 55 years and met the inclusion criteria after obtaining ethical approval from Rivers state teaching hospital ethics committee. The women were divided into four subgroups: outpatients, immunocompromised patients (HIV clinic patients), pregnant women, and healthy volunteers. Well-structured questionnaires were used to obtain data on risk factors of chlamydiosis on consultancy basis. Healthy volunteers were those who showed no symptoms otherwise regarded as apparently healthy volunteers. The data obtained were analyzed using Chlamydia trachomatis IgG Enzyme Immunoassay test kit. Results: Out of the 450 samples examined, 45 (10%) tested positive to chlamydia antibodies with the highest prevalence among the immunodeficiency virus subjects (4.2%) and this was followed by the outpatient (2.4%) and healthy volunteers (2.2%). Pregnant women had the lowest seroprevalence rate of 1.1%. The study further revealed that about 78% of the study population heard about chlamydiosis while 22% had no knowledge of it. Statistically, there was no association (p=.06) between awareness parameters and sero-prevalence of chlamydiosis. Similarly, there was no association (p=.08) between person hygiene parameters and sero-prevalence of chlamydiosis. The study equally revealed that there was an association between self-screening for sexually transmitted infection and ever screened for Chlamydia trachomatis as life styles for chlamydiosis (p=.05). Conclusion: The results of this study indicate a strong correlation between self-screening for Sextually transmitted infections and chlamydia infection and those who have ever had a Chlamydia trachomatis screening. In order to lessen the harmful effects chlamydiosis has on society, particularly among those who are of reproductive age, awareness of the risk factor associated with the disease should also be increased.

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Comparison of Elisa and Rapid Immunochromatographic Tests in Diagnosis of Toxoplasmosis in Port Harcourt, Nigeria

Cited by 12 publications

References 9 publications

Prevalence and co-infection of Toxoplasma gondii and Human Immunodeficiency Virus (HIV) infection among women of child-bearing age in Osun state Nigeria

Prevalence and co-infection of Toxoplasma gondii and Human Immunodeficiency Virus (HIV) infection among women of child-bearing age in Osun state Nigeria

Performance of immunochromatographic and immunoenzymatic techniques in the diagnosis of toxoplasmosis in pregnant women in Cameroon: need for harmonization

The Sero-prevalence and Risk Factors of Chlamydiosis among Women of Reproductive Age in Port Harcourt, Nigeria

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