Comparison of eMAG™ versus NucliSENS® EasyMAG® performance on clinical specimens

Garcia, Magali; Chessa, Céline; Bourgoin, A.; Giraudeau, G.; Plouzeau, Chloé; Agius, G.; Lévêque, Nicolas; Beby‐Defaux, Agnès

doi:10.1016/j.jcv.2017.01.004

Cited by 8 publications

(6 citation statements)

References 21 publications

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“…These differences could be due to the various properties of viruses, including the presence of an envelope, the type of genome, or the size of the virions. Yang et al showed a better performance of RNA virus recovery with EasyMag (identical silica extraction technology and similar performance to that of Emag [ 45 ]) compared to the MagNA Pure Compact. They explained this difference by a possible RNA degradation or by an ineffective binding of RNA to the magnetic beads [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Nucleic Acid Extraction Methods for a Viral Metagenomics Analysis of Respiratory Viruses

et al. 2020

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Viral metagenomics next-generation sequencing (mNGS) is increasingly being used to characterize the human virome. The impact of viral nucleic extraction on virome profiling has been poorly studied. Here, we aimed to compare the sensitivity and sample and reagent contamination of three extraction methods used for viral mNGS: two automated platforms (eMAG; MagNA Pure 24, MP24) and the manual QIAamp Viral RNA Mini Kit (QIAamp). Clinical respiratory samples (positive for Respiratory Syncytial Virus or Herpes Simplex Virus), one mock sample (including five viruses isolated from respiratory samples), and a no-template control (NTC) were extracted and processed through an mNGS workflow. QIAamp yielded a lower proportion of viral reads for both clinical and mock samples. The sample cross-contamination was higher when using MP24, with up to 36.09% of the viral reads mapping to mock viruses in the NTC (vs. 1.53% and 1.45% for eMAG and QIAamp, respectively). The highest number of viral reads mapping to bacteriophages in the NTC was found with QIAamp, suggesting reagent contamination. Our results highlight the importance of the extraction method choice for accurate virome characterization.

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Section: Discussionmentioning

confidence: 99%

Comparison of Nucleic Acid Extraction Methods for a Viral Metagenomics Analysis of Respiratory Viruses

et al. 2020

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“…Total nucleic acid was extracted using the NucliSens EASYMAG system (Marcy l’Etoile, France). 9 , 10 We used reverse transcriptase quantitative PCR to identify RSV following the protocol developed by the respiratory viruses branch at the US Centers for Disease Control and Prevention (CDC). 11 We tested each sample for the constitutive human enzyme RNAseP to show adequate sampling and the absence of PCR inhibitors.…”

Section: Methodsmentioning

confidence: 99%

Infant deaths from respiratory syncytial virus in Lusaka, Zambia from the ZPRIME study: a 3-year, systematic, post-mortem surveillance project

Gill¹,

Mwananyanda²,

MacLeod³

et al. 2022

The Lancet Global Health

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Summary Background Respiratory syncytial virus (RSV) is the leading cause of acute lower respiratory tract infections and a key driver of childhood mortality. Previous RSV burden of disease estimates used hospital-based surveillance data and modelled, rather than directly measured, community deaths. Given this uncertainty, we conducted a 3-year post-mortem prevalence study among young infants at a busy morgue in Lusaka, Zambia—the Zambia Pertussis RSV Infant Mortality Estimation (ZPRIME) study. Methods Infants were eligible for inclusion if they were aged between 4 days and less than 6 months and were enrolled within 48 h of death. Enrolment occurred mainly at the University Teaching Hospital of the University of Zambia Medical School (Lusaka, Zambia), the largest teaching hospital in Zambia. We extracted demographic and clinical data from medical charts and official death certificates, and we conducted verbal autopsies with the guardian or next of kin. RSV was identified using reverse transcriptase quantitative PCR and stratified by age, time of year, and setting (community vs facility deaths). By combining the PCR prevalence data with syndromic presentation, we estimated the proportion of all infant deaths that were due to RSV. Findings The ZPRIME study ran from Aug 31, 2017, to Aug 31, 2020, except for from April 1 to May 6, 2020, during which data were not collected due to restrictions on human research at this time (linked to COVID-19). We enrolled 2286 deceased infants, representing 79% of total infant deaths in Lusaka. RSV was detected in 162 (7%) of 2286 deceased infants. RSV was detected in 102 (9%) of 1176 community deaths, compared with 10 (4%) of 236 early facility deaths (<48 h from admission) and 36 (5%) of 737 late facility deaths (≥48 h from admission). RSV deaths were concentrated in infants younger than 3 months (116 [72%] of 162 infants), and were clustered in the first half of each year and in the poorest and most densely populated Lusaka townships. RSV caused at least 2·8% (95% CI 1·0–4·6) of all infant deaths and 4·7% (1·3–8·1) of community deaths. Interpretation RSV was a major seasonal cause of overall infant mortality, particularly among infants younger than 3 months of age. Because most RSV deaths occurred in the community and would have been missed through hospital-based surveillance, the global burden of fatal RSV has probably been underestimated. Funding Bill & Melinda Gates Foundation.

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“…Altogether, with minor or no differences in the LOD, all three platforms presented very similar performance. As the three platforms are technically similar, especially eMAG and easyMAG, these analytical assessments were expected as previously described [9].…”

Section: Discussionmentioning

confidence: 99%

“…The performance of the semi-automated easyMAG system has been extensively investigated; the platform was shown to efficiently extract viral nucleic acid [7,8]. In a study that compared the performance of the new eMAG and the easyMAG systems, eMAG was shown to perform equally well on several types of clinical samples including respiratory samples [9]. However, no large-scale study has simultaneously compared the performance of the eMAG to that of easyMAG and MagNA PURE 96 in nucleic acid extraction from respiratory samples.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the new fully automated extraction platform eMAG to the MagNA PURE 96 and the well-established easyMAG for detection of common human respiratory viruses

et al. 2019

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Respiratory viral infections constitute the majority of samples tested in the clinical virology laboratory during the winter season, and are mainly diagnosed using molecular assays, namely real-time PCR (qPCR). Therefore, a high-quality extraction process is critical for successful, reliable and sensitive qPCR results. Here we aimed to evaluate the performance of the newly launched eMAG compared to the fully automated MagNA PURE 96 (Roche, Germany) and to the semi-automated easyMAG (bioMerieux, France) extraction platforms. For this analysis, we assessed and compared the analytic and clinical performance of the three platforms, using 262 archived respiratory samples positive or negative to common viruses regularly examined in our laboratory (influenza A, B, H1N1pdm, Respiratory Syncytial Virus (RSV), human Metapneumovirus (hMPV), parainfluenza-3, adenovirus and negative samples). In addition, quantitated virus controls were used to determine the limit of detection of each extraction method. In all categories tested, eMAG results were comparable to those of the easyMAG and MagNa PURE 96, highly sensitive for all viruses and over 98% clinical specificity and sensitivity for all viruses tested. Together with its high level of automation, the bioMerieux eMAG is a high-quality extraction platform enabling effective molecular analysis and is mostly suitable for medium-sized laboratories.

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Comparison of eMAG™ versus NucliSENS® EasyMAG® performance on clinical specimens

Abstract: The two platforms showed comparable performance on the types of clinical specimen tested. With higher automation and throughput than easyMAG, the eMAG™ platform is likely to be advantageous for laboratories performing a large number of molecular analyses.

Cited by 8 publications

References 21 publications

Comparison of Nucleic Acid Extraction Methods for a Viral Metagenomics Analysis of Respiratory Viruses

Comparison of Nucleic Acid Extraction Methods for a Viral Metagenomics Analysis of Respiratory Viruses

Infant deaths from respiratory syncytial virus in Lusaka, Zambia from the ZPRIME study: a 3-year, systematic, post-mortem surveillance project

Comparison of the new fully automated extraction platform eMAG to the MagNA PURE 96 and the well-established easyMAG for detection of common human respiratory viruses

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