BACKGROUND: Water is critical to the production of crops, especially when faced with seasonal drought or freshwater scarcity. We compared the effect of negative pressure irrigation (NPI) on water use efficiency (WUE), nutrient uptake, yield and quality of Brassica chinensis L. using a greenhouse plot experiment. Three different water supply pressures (−5, −10 and −15 kPa), and a conventional irrigation (CK) treatment, were arranged in a randomized design with three replications. RESULTS: Our results suggest that plant height, leaf area, number of leaves and ratio of root to shoot were significantly correlated with water supply pressure. Specifically, our results show that B. chinensis L. yield was increased 50% with NPI versus CK. Water supply pressure had a significant effect on N and P nutrient uptake and no significant effect on K. The average concentration of vitamin C was greatest with −5 kPa treatment and consecutively declined. According to our results, NPI can save up to 36.8% of water used and improve WUE by 61.3% during growth of B. chinensis L. Our results suggest that the optimum irrigation management strategy is −5 kPa treatment.CONCLUSION: NPI versus CK can provide more stable irrigation water and retain soil moisture during plant growth, resulting in an increased WUE and yield with suitable water supply pressure. While our results suggest that NPI can enhance B. chinensis L. yield and perhaps also quality, future research should explore the mechanism of NPI in relation to yield and water use efficiency.