Comparison of Endothelial Differentiation Capacities of Human and Rat Adipose-Derived Stem Cells

Orbay, Hakan; Devi, Kamaljit; Williams, Priscilla A.; Dehghani, Tima; Silva, Eduardo A.; Sahar, David E.

doi:10.1097/prs.0000000000002791

Cited by 16 publications

(8 citation statements)

References 37 publications

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“…ADSCs from passages 3 and 4 were reported to be used in regenerative medicine (13)(14)(15). The results of this study also indicated no significant difference in spontaneous and induced adipogenic differentiation potential between cells of passages 3 and 4.…”

Section: Discussionsupporting

confidence: 59%

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Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages

Yang

Zhang

2018

Mol Med Report

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Primary adipose-derived stem cells (ADSCs) are a mixture of cell types including preadipocytes having the ability to spontaneously differentiate into adipocytes. The aim of the present study was to compare the spontaneous adipogenic differentiation potential of ADSCs at different passages to determine whether it decreased with continuous cell passages. Mouse ADSCs (mADSCs) were harvested and cells from passages 1 to 5 were used for experiments. The proliferation of mADSCs at different passages was tested using the cell counting kit‑8 assay. Reverse transcription‑quantitative polymerase chain reaction was used to determine relative mRNA expression levels of CCAAT/enhancer binding protein α (Cebpa and C/EBPα) and peroxisome proliferator‑activated receptor γ (Pparg and PPARγ), and western blot analysis was used to investigate C/EBPα and PPARγ protein expression. The cells were cultured using DMEM. The fixed cells were then stained using Oil Red O on days 14 and 28, and the obtained extracted dye was monitored for absorbance. The 510 nm absorbance from passages 1 to 5 was observed to be statistically different. The relative expression levels of Cebpa and Pparg for mADSCs from passage 1 were significantly higher when compared with those for mADSCs from passages 2 to 5 on days 3, 5 and 7. However, no difference was identified in the expression levels of proteins C/EBPα and PPARγ for different passages. Although the mRNA expression levels of Pparg from passages 4 to 5 were significantly higher when compared with those from passage 1, the results of Oil Red O absorbance, mRNA expression levels of Cebpa, and the protein expression levels of C/EBPα and PPARγ exhibited no difference between mADSCs from passages 1 to 5 when cultured with induced adipogenic differentiation medium. Therefore, it was concluded that the spontaneous adipogenic differentiation potential of ADSCs decreased with continuous cell passages.

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Section: Discussionsupporting

confidence: 59%

“…The spontaneous adipogenic differentiation of ADSCs was assumed to decrease when the cells were passaged serially. ADSCs from passages 3 and 4 were reported to be used in regenerative medicine (13)(14)(15). Krähenbühl et al (16) reported that cell morphology changed and growth slowed down when extended to passages 6 and beyond.…”

Section: Introductionmentioning

confidence: 99%

Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages

Yang

Zhang

2018

Mol Med Report

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“…These multipotent cells can be harvested from various tissues, such as adipose tissue, bone marrow, periosteum, and synovium [16][17][18][19] . Owing to their abundant source and easy access, adipose-derived stem cells (ASCs) have become the principal source of MSCs, with multidirectional differentiation properties into adipocytes, osteoblasts, chondrocytes, and other lineages, which have been investigated by experimental studies in vitro or in vivo [20][21][22] . ASCs are routinely harvested by the enzymatic method and then expanded for several generations to sufficient quantity, with significant aging and a decline in multipotency as well as the destruction of the vascular matrix 23 .…”

Section: Introductionmentioning

confidence: 99%

Autologous Micro-Fragmented Adipose Tissue as Stem Cell-Based Natural Scaffold for Cartilage Defect Repair

Yang

et al. 2019

Cell Transplant

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Osteoarthritis (OA) poses a tough challenge worldwide. Adipose-derived stem cells (ASCs) have been proved to play a promising role in cartilage repair. However, enzymatic digestion, ex vivo culture and expansion, with significant senescence and decline in multipotency, limit their application. The present study was designed to obtain micro-fragmented adipose tissue (MFAT) through gentle mechanical force and determine the effect of this stem cell-based natural scaffold on repair of full-thickness cartilage defects. In this study, ASCs sprouted from MFAT were characterized by multi-differentiation induction and flow cytometry. Scratch and transwell migration assays were operated to determine whether MFAT could promote migration of chondrocytes in vitro. In a rat model, cartilage defects were created on the femoral groove and treated with intra-articular injection of MFAT or PBS for 6 weeks and 12 weeks ( n = 12). At the time points, the degree of cartilage repair was evaluated by histological staining, immunohistochemistry and scoring, respectively. Two unoperated age-matched animals served as native controls. ASCs derived from MFAT possessed properties to differentiate into adipocytes, osteocytes and chondrocytes, with expression of mesenchymal stem cell markers (CD29, 44, 90) and no expression of hematopoietic markers (CD31, 34, 45). In addition, MFAT could significantly promote migration of chondrocytes. MFAT-treated defects showed improved macroscopic appearance and histological evaluation compared with PBS-treated defects at both time points. After 12 weeks of treatment, MFAT-treated defects displayed regular surface, high amount of hyaline cartilage, intact subchondral bone reconstruction and corresponding formation of type I, II, and VI collagen, which resembled the normal cartilage. This study demonstrates the efficacy of MFAT on cartilage repair in an animal model for the first time, and the utility of MFAT as a ready-to-use therapeutic alternative to traditional stem cell therapy.

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“…Moreover, they are ideal candidates for use as vehicles for gene therapy 1 . Thus far, ECs have been isolated from various sources, such as from peripheral blood mononuclear cells 2 , bone marrow mononuclear cells 3 , cardiac progenitors 4 , adipose-derived stem cells 5 and umbilical cord blood 6 . However, ECs from these adult sources are reported to be difficult to identify, isolate and expand in culture 7 .…”

Section: Introductionmentioning

confidence: 99%

FLI1 and PKC co-activation promote highly efficient differentiation of human embryonic stem cells into endothelial-like cells

Zhao

et al. 2018

Cell Death Dis

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Rationale-endothelial cells (ECs) play important roles in various regeneration processes and can be used in a variety of therapeutic applications, such as cardiac regeneration, gene therapy, tissue-engineered vascular grafts and prevascularized tissue transplants. ECs can be acquired from pluripotent and adult stem cells. To acquire ECs from human embryonic stem cells (hESCs) in a fast, efficient and economic manner. We established a conditional overexpression system in hESCs based on 15 transcription factors reported to be responsible for hematopoiesis lineage. Among them, only overexpression of FLI1 could induce hESCs to a hematopoietic lineage. Moreover, simultaneous overexpression of FLI1 and activation of PKC rapidly and efficiently induced differentiation of hESCs into induced endothelial cells (iECs) within 3 days, while neither FLI1 overexpression nor PKC activation alone could derive iECs from hESCs. During induction, hESCs differentiated into spindle-like cells that were consistent in appearance with ECs. Flow cytometric analysis revealed that 92.2–98.9% and 87.2–92.6% of these cells were CD31+ and CD144+, respectively. Expression of vascular-specific genes dramatically increased, while the expression of pluripotency genes gradually decreased during induction. iECs incorporated acetylated low-density lipoproteins, strongly expressed vWF and bound UEA-1. iECs also formed capillary-like structures both in vitro and in vivo. RNA-seq analysis verified that these cells closely resembled their in vivo counterparts. Our results showed that co-activation of FLI1 and PKC could induce differentiation of hESCs into iECs in a fast, efficient and economic manner.

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Comparison of Endothelial Differentiation Capacities of Human and Rat Adipose-Derived Stem Cells

Cited by 16 publications

References 37 publications

Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages

Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages

Autologous Micro-Fragmented Adipose Tissue as Stem Cell-Based Natural Scaffold for Cartilage Defect Repair

FLI1 and PKC co-activation promote highly efficient differentiation of human embryonic stem cells into endothelial-like cells

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