Comparison of enhanced chemiluminescence and microparticle enzyme immunoassay for the measurement of hepatitis B surface antibody

McCartney, R.A.; Harbour, Jake C.; Roome, A.P.C.H.; Caul, E.O.

doi:10.1016/0264-410x(93)90383-9

Cited by 8 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In 3 cases, all anti-HBs were in 10 to 100 mIU/mL range while HBsAg was elevated. The real reason for this phenomenon was unclear but may be associated with infection of different HBV subtypes, transient HBsAg positive (mostly found at 6 days after hepatitis B immunization) or false positivity due to measurement methods 2,13-15…”

Section: Discussionmentioning

confidence: 99%

“…Detection of serological markers has evolved from a cumbersome and time-consuming procedure by manual radioimmunoassay or enzyme-linked immunosorbent assay (ELISA) to procedures with systems that partially or fully automate the process. Electrochemiluminescence immunoassay (ECLIA) is a new quantitatively analysis technique with improved both sensitivity and measurement ranges, shortened reaction period (approximately 18 minutes) and random-access capabilities for specimen, has been gradually applied to detect these antibodies and antigens 2-4…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Distribution Characteristics of Hepatitis B Serological Markers in Hospitalized Children and Adolescents in Zhejiang, China between 2006 and 2010

Chen¹,

Shen²,

Xiang³

2011

Gut Liver

View full text Add to dashboard Cite

Background/AimsTo investigate serological patterns of hepatitis B based on electrochemiluminescent immunoassays and the distribution characteristics of these patterns in hospitalized children and adolescents in Zhejiang, China between 2006 and 2010.MethodsFive serological markers, including hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), antibody to hepatitis B surface antigen (anti-HBs), antibody to hepatitis B e antigen (anti-HBe), and antibody to hepatitis B c antigen (anti-HBc), were chosen as a routine panel to monitor hepatitis B virus (HBV) infection and vaccination efficacy. A total of 33,187 children (21,187 boys and 12,000 girls) were selected using the following exclusion criteria: a previous diagnosis of hepatitis, age >16 years or an address outside of Zhejiang.ResultsThe average HBV vaccination coverage rates among 20,766 boys and 11,782 girls were 98.62% and 98.68%, respectively. Seventeen serological patterns of hepatitis B were found, and the dominant pattern was 'anti-HBs (+) alone' (62.03%) followed by 'negative pattern' (23.46%). The rates of the other 15 patterns ranged from 8.14% to 0.003%. Of 236 HBsAg-positive patients, the overall rate of seropositivity was 0.71%. The anti-HBs levels were grouped into 3 ranges (10-100 mIU/mL, 100-1,000 mIU/mL, and >1,000 mIU/mL) for all anti-HBs-positive children (36.08%, 43.43%, and 20.49%, respectively).ConclusionsA low HBsAg carrier rate and a relatively high anti-HBs positive rate are present in hospitalized children and adolescents in Zhejiang. The distribution of serological patterns is associated with age but is mostly independent of gender.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Distribution Characteristics of Hepatitis B Serological Markers in Hospitalized Children and Adolescents in Zhejiang, China between 2006 and 2010

Chen¹,

Shen²,

Xiang³

2011

Gut Liver

View full text Add to dashboard Cite

show abstract

“…Agreement was demonstrated with the ADVIA Centaur result with seven samples and with the Abbott AxSYM result with 10 samples. The difficulties of comparing anti-HBs results from different manufacturers has been previously demonstrated by Heijtink et al (2002) and McCartney et al (1993). The conclusion of the latter group that levels are dependent on the test reagents is borne out by this present study since the three assays used have different antigen sources.…”

Section: Hbv Markersmentioning

confidence: 50%

Comparison of the ADVIA centaur® and Abbott AxSYM immunoassay systems for a routine diagnostic virology laboratory

Taylor¹,

Pickard²,

Gammie

2004

Journal of Clinical Virology

View full text Add to dashboard Cite

“…Other studies have also shown higher values with MEIA than with EIA or chemoluminescence (5,7), especially in vaccinated patients (10). Other studies have also shown higher values with MEIA than with EIA or chemoluminescence (5,7), especially in vaccinated patients (10).…”

Section: Discussionmentioning

confidence: 93%

Technical Note

1996

CCLM

View full text Add to dashboard Cite

The Roche Cobas Core automated enzyme immunoassay of antibodies against hepatitis B virus surface antigen (anti-HBs) was evaluated using a protocol complying with the Societe Frangaise de Biologie Clinique guidelines. Results showed good precision (CV below 6 and 9% for intra-and inter-assay precision) and accuracy (according to the Paul-Ehrlich-Institute standard); the detection limit of the test was 2 IU/1. The manufacturer's specifications were confirmed and a satisfactory correlation (r = 0.901) was found with an automated method (IMx, Abbott) used for comparison.

show abstract

Comparison of enhanced chemiluminescence and microparticle enzyme immunoassay for the measurement of hepatitis B surface antibody

Cited by 8 publications

References 6 publications

Distribution Characteristics of Hepatitis B Serological Markers in Hospitalized Children and Adolescents in Zhejiang, China between 2006 and 2010

Distribution Characteristics of Hepatitis B Serological Markers in Hospitalized Children and Adolescents in Zhejiang, China between 2006 and 2010

Comparison of the ADVIA centaur® and Abbott AxSYM immunoassay systems for a routine diagnostic virology laboratory

Technical Note

Contact Info

Product

Resources

About