Comparison of enzymatic properties between hPADI2 and hPADI4

Citrullinated fibrinogen shows defects in FPA and FPB release and fibrin polymerization catalyzed by thrombin

“…These analyses demonstrated that both the Aα-and Bβ-chains of fibrinogen were strongly citrullinated and that the γ-chain was weakly citrullinated. Our results agree with Nakayama-Hamada's analysis [10]. Interestingly, the citrullinated Aα-chain had a markedly higher molecular mass than the normal Aα-chain under reducing…”

“…These observations have been already reported by others [2,[9][10][11]. Although the degree of our and Zhao's observations [11] were marked, Masson-Bessiere's [2] was medium and Takizawa's [9] and Nakayama-Hamada's [10] were mild.…”

“…These observations have been already reported by others [2,[9][10][11]. Although the degree of our and Zhao's observations [11] were marked, Masson-Bessiere's [2] was medium and Takizawa's [9] and Nakayama-Hamada's [10] were mild. Citrullination of arginine residues might lead to slower mobility shifts during SDS-PAGE analysis, and the degree of this change is dependent on the number of citrullinated residues on the Aα-chain because Arg makes up 40 of its 610 residues [10].…”

“…A similar mobility shift in citrullinated Aα-chains has already been observed by other researchers [2,[9][10][11] To demonstrate the citrullination of fibrinogen, blots were visualized by an anti-modified citrulline detection kit (Upstate, Waltham, MA) [6]. Fibrinogen (under nonreducing conditions) and the Aα-and Bβ-chains of C-Fbg (under reducing conditions)…”

“…Citrullination at Aα-16Arg and Bβ-14Arg have been already reported by Nakayama-Hamada et al [10]. Most recently, Nakayama-Hamada et al [16] reported that no FPA or FPB release and no polymerization of citrullinated fibrinogen were observed after thrombin stimulation.…”