Comparison of enzyme immunoassays detecting Helicobacter pylori specific IgG in serum and saliva with endoscopic and biopsy findings in patients with dyspepsia

El-Mekki, A.; Kumar, Ashok; A, Ba; Al-Ammari, O; Moosa, Riyad A.; Quli, Syed K.; Ahmed, M-Elbagir K

doi:10.4103/0255-0857.81793

Cited by 13 publications

(3 citation statements)

References 17 publications

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“…During a survey, the sensitivity and specificity of salivary antibody for detection of H. pylori were 80.9% and 95.3% respectively, that the specificity was significantly higher than ours ( 24 ). El-Mekki et al found no significant difference between serum and salivary antibody levels regarding endoscopic findings, which was concordant with our study ( 23 ). In this research, serum and salivary antibodies for diagnosis of H. pylori were acceptable with no statistical difference, while El-Fakhfakh et al found that the salivary antibodies were more sensitive and specific than serum antibodies ( 5 ).…”

Section: Discussionsupporting

confidence: 93%

“…Rahman et al reported sensitivity and specificity of serum IgG against H. pylori about 96.7% and 42.8% respectively, that the sensitivity was more than our findings and the specificity was lesser than our results ( 22 ). In current work, the salivary IgG revealed a sensitivity and specificity of 85% and 82% but El-Mekki et al indicated a high sensitivity (95%) and low specificity (70%) of ELISA test ( 23 ). In a survey of Krishnaswamy et al, sensitivity (79.31%) of salivary IgG was acceptable, but specificity (63.64%) was low ( 1 ).…”

Section: Discussioncontrasting

confidence: 55%

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Comparison of the serum and salivary antibodies to detect gastric Helicobacter pylori infection in Kashan (Iran)

et al. 2017

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Background and aimHelicobacter pylori (H. pylori) is an important and common contagious human pathogen which may cause peptic ulcer and also gastric cancer. The definite diagnosis of it is made through invasive tests. Recently, non-invasive tests including serologic tests of serum and saliva have been conducted for diagnosis of H. pylori infection. In this research, the diagnostic values of serum and salivary serology were compared together to use salivary anti-H. pylori test as an alternative method in the future.MethodsDuring this prospective case-control study on patients who were candidates for endoscopy and gastric biopsy from March 2015 to April 2016 in Shahid Beheshti hospital, Kashan, Iran, serum and salivary samples were obtained for measurement of Immunoglobulin G (IgG) antibody levels against H. pylori by enzyme-linked immunosorbent assay (ELISA). Histopathology was the gold standard test. Statistical analysis was performed by SPSS software version 16. Statistical tests included Kolmogorov-Smirnov, independent-samples t-test, Chi-square, Mann-Whitney U, Kruskal-Wallis, McNemar and correlation.ResultsOf 123 patients, sixty-one patients (49.6%) were H. pylori-positive according to histology. The median levels of anti-H. pylori antibodies in serum (p<0.001) and saliva (p<0.001) of H. pylori-positive cases were significantly higher than H. pylori-negative cases. Sensitivity, specificity, positive likelihood ratio, negative likelihood ratio and accuracy of serologic tests in serum were 75%, 79%, 3.5, 0.3, 77% and for saliva were 85%, 82%, 4.7, 0.18, 84% respectively.ConclusionDiagnostic values of salivary ELISA are comparable to serum ELISA and can be used as an alternative modality for diagnosis of H. pylori infection.

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Section: Discussionsupporting

confidence: 93%

Section: Discussioncontrasting

confidence: 55%

Comparison of the serum and salivary antibodies to detect gastric Helicobacter pylori infection in Kashan (Iran)

et al. 2017

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“…7 - 8 Saliva H. pylori antibody testing and stool antigen testing can readily be manipulated, as many studies have reported. 9 , 10 However, it was seldom reported regarding saliva H. pylori antigen testing. 11 H. pylori Saliva Test Cassette (HPS) is to use monoclonal antibody to specifically identify urease in saliva after H. pylori infection.…”

Section: Introductionmentioning

confidence: 99%

Application of Saliva Test Cassette to detect oral infection with Helicobacter pylori among college students in Yancheng

Zhang

2015

Pak J Med Sci

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Objective:To investigate the incidence of oral infection with Helicobacter pylori (H. pylori) and identify related epidemiological factors among freshmen of four colleges in Yancheng.Methods:The data, scored positive or negative, were collected on 160 individuals who had been diagnosed by H. pylori Saliva Test Cassette (HPS) during October 2013 to October 2014. H. pylori Saliva Test Cassette (HPS) is to use colloidal gold technique to specifically identify urease in saliva. A standard questionnaire, with variables including sex, educational degree of parents etc., was used in the subjects. Statistical data of diagnostic test were analyzed by SPSS17.0 software.Results:Out of 160, 82 subjects were detected positive and 78 were negative. In univariate analysis, dental plaque, family history of stomach diseases, habit of washing hands before meals and habit of brushing teeth twice daily were associated negatively with H. pylori infection. Multivariate logistic regression analysis showed that dental plaque and family history of stomach diseases were the risk factors which may be associated with H. pylori infection.Conclusions:Dental plaque and family history of gastric diseases were risk factors of oral H. pylori infection. It is vital for the prevention of H. pylori infection to focus on health education and oral hygiene, and avoid transmission by oral-oral route as well.

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Non-invasive diagnostic tests forHelicobacter pyloriinfection

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Takwoingi

Siddique

et al. 2018

Cochrane Database of Systematic Reviews

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In people without a history of gastrectomy and those who have not recently had antibiotics or proton ,pump inhibitors, urea breath tests had high diagnostic accuracy while serology and stool antigen tests were less accurate for diagnosis of Helicobacter pylori infection.This is based on an indirect test comparison (with potential for bias due to confounding), as evidence from direct comparisons was limited or unavailable. The thresholds used for these tests were highly variable and we were unable to identify specific thresholds that might be useful in clinical practice.We need further comparative studies of high methodological quality to obtain more reliable evidence of relative accuracy between the tests. Such studies should be conducted prospectively in a representative spectrum of participants and clearly reported to ensure low risk of bias. Most importantly, studies should prespecify and clearly report thresholds used, and should avoid inappropriate exclusions.

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Comparison of enzyme immunoassays detecting Helicobacter pylori specific IgG in serum and saliva with endoscopic and biopsy findings in patients with dyspepsia

Cited by 13 publications

References 17 publications

Comparison of the serum and salivary antibodies to detect gastric Helicobacter pylori infection in Kashan (Iran)

Comparison of the serum and salivary antibodies to detect gastric Helicobacter pylori infection in Kashan (Iran)

Application of Saliva Test Cassette to detect oral infection with Helicobacter pylori among college students in Yancheng

Non-invasive diagnostic tests forHelicobacter pyloriinfection

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