Comparison of Enzyme-Linked Immunosorbent Assay, Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust

Abstract

A sample preparation method was developed for the screening of deoxynivalenol (DON) in wheat and wheat dust. Extraction was carried out with water and was successful due to the polar character of DON. For detection, an enzyme-linked immunosorbent assay (ELISA) was compared to the sensor-based techniques of surface plasmon resonance (SPR) and biolayer interferometry (BLI) in terms of sensitivity, affinity and matrix effect. The matrix effects from wheat and wheat dust using SPR were too high to further use this screenings method. The preferred ELISA and BLI methods were validated according to the criteria established in Commission Regulation 519/2014/EC and Commission Decision 2002/657/EC. A small survey was executed on 16 wheat lots and their corresponding dust samples using the validated ELISA method. A linear correlation (r = 0.889) was found for the DON concentration in dust versus the DON concentration in wheat (LOD wheat: 233 μg/kg, LOD wheat dust: 458 μg/kg).

Mycotoxin Analysis of Grain via Dust Sampling: Review, Recent Advances and the Way Forward: The Contribution of the MycoKey Project

“…In parallel, studies were carried out on the sampling of wheat dust with a laboratory dust collection system and subsequent analysis of DON by LC-MS/MS or ELISA within the European FP7 MycoHunt project ("Rapid biosensor for the detection of mycotoxin in wheat") [45,48,49]. The dust was produced from the wheat samples by the use of a dust collection facility.…”

“…In these studies, dust sample particles <50 µm were collected by sieving light fractions (<2500 µm). A linear correlation between the DON content in wheat and in corresponding dust was found either in a set of 12 wheat samples analyzed by LC-MS/MS (R 2 = 0.94) [45], or in a set of 16 wheat samples (R 2 = 0.89) analyzed by ELISA [48]. The ratio between DON contamination in dust and wheat was evaluated by the value of slopes of linear regression (13 and 5, respectively).…”

Decontamination of Mycotoxin-Contaminated Feedstuffs and Compound Feed

“…Dust removal can be efficient in removing mycotoxins. As shown by different studies, mycotoxins are often accumulated in the grain dust, and exposure to it can have toxic effects on animals and humans [26,27]. Efficient dust collection systems and separation systems can be efficient in mycotoxin redirection into fine fractions which are excluded from the raw materials or final products.…”

Mechanism of Paeoniae Radix Alba in the Treatment of Non-alcoholic Fatty Liver Disease Based on Sequential Metabolites Identification Approach, Network Pharmacology, and Binding Affinity Measurement

“…When the analyte molecules in a liquid sample were in contact with the ligands, changes in the refractive index (RI) at the sensor surface were produced, which can be measured by the optical reader ( 27 , 28 ). Hence, the major advantage of SPR is that the sample can be detected in real time and without the need of labeling ( 29 , 30 ). Another advantage is that SPR can directly and specifically capture the bioactive candidates from complex matrices ( 31 ).…”