Comparison of Enzyme-Linked Immunosorbent Assay, Western Blotting, Microagglutination, Indirect Immunofluorescence Assay, and Flow Cytometry for Serological Diagnosis of Tularemia

Porsch-Özçürümez, Mustafa; Kischel, Nele; Priebe, Heidi; Splettstößer, W.; Finke, Ernst-Jürgen; Grunow, Roland

doi:10.1128/cdli.11.6.1008-1015.2004

Cited by 106 publications

(82 citation statements)

References 33 publications

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“…This may be a mobile laboratory which is brought in or a local labora tory which is additionally equipped for diagnosing tularaemia. A field laboratory for tularae mia should be able to perform microagglutination and/or ELISA for antigen and antibody detection (Syrjälä et al, 1986;Sjöstedt et al, 1997;Grunow et al, 2000;Porsch-Ozcurumez et al, 2004). In addition, simple instruments for genetic detection of pathogens under field conditions and biosensors are currently under development.…”

Section: Laboratory Methods Suitable Under Field Conditionsmentioning

confidence: 99%

Increase in Human Cases of Tularemia — Colorado, Nebraska, South Dakota, and Wyoming, January–September 2015

Pedati¹,

House²,

Hancock-Allen³

et al. 2015

MMWR Morb. Mortal. Wkly. Rep.

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Section: Laboratory Methods Suitable Under Field Conditionsmentioning

confidence: 99%

Increase in Human Cases of Tularemia — Colorado, Nebraska, South Dakota, and Wyoming, January–September 2015

Pedati¹,

House²,

Hancock-Allen³

et al. 2015

MMWR Morb. Mortal. Wkly. Rep.

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“…This type of analysis was performed in some clinical studies (Sumida et al, 1992;Porsch-Ozcurumez et al, 2004) and it was used for microbiological and immunological research purposes (Abd et al, 2003;Chen et al, 2005). However, the detection of F. tularensis or a valid diagnostic system based on flow cytometry still remain a challenge.…”

Section: Classical Immuno-assaysmentioning

confidence: 99%

“…A large testing of 6 632 serum samples was performed to compare ELISA, western blot, flow cytometry and immunofluorescence in which western blot and flow cytometry provided slightly better results. However, some other technical parameters such as the amount of samples necessary for tests confirmed ELISA as a favourable choice (Porsch-Ozcurumez et al, 2004).…”

Section: Classical Immuno-assaysmentioning

confidence: 99%

Current and emerging assays for Francisella tularensis detection: a review

Pohanka¹,

Hubálek²,

Neubauerová³

et al. 2008

Vet. Med. - Czech

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This paper presents an overview of methods for detection and identification of the pathogenic bacterium Francisella tularensis such as cultivation tests, enzyme-linked immunosorbent assays, flow cytometry, polymerase chain reaction, immunosensor, microarray, mass spectrometry, and chromatography. Included references are chosen according to their practical importance or perspectives for the future.

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“…The reactive antigens in the MA are not well understood, and a test based on defined antigens might be preferable. When F. tularensis lipopolysaccharide is used in an enzyme-linked immunosorbent assay (ELISA) format, the specificity of the assay is comparable to that of the MA (20). Outer membrane antigens have also been used for detection of antibodies (23,24).…”

mentioning

confidence: 99%

“…The sensitivity of the MA is usually 100% by 1 week after infection. Uninfected controls usually show titers below 1/16, while exposed individuals show titers of 1/64 or above (18,20). As there are reports of potential cross-reactivity with antibodies against other bacteria, such as Brucella, Yersinia, and Proteus spp.…”

mentioning

confidence: 99%

Towards Development of Improved Serodiagnostics for Tularemia by Use of Francisella tularensis Proteome Microarrays

et al. 2016

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e Tularemia in humans is caused mainly by two subspecies of the Gram-negative facultative anaerobe Francisella tularensis: F. tularensis subsp. tularensis (type A) and F. tularensis subsp. holarctica (type B). The current serological test for tularemia is based on agglutination of whole organisms, and the reactive antigens are not well understood. Previously, we profiled the antibody responses in type A and B tularemia cases in the United States using a proteome microarray of 1,741 different proteins derived from the type A strain Schu S4. Fifteen dominant antigens able to detect antibodies to both types of infection were identified, although these were not validated in a different immunoassay format. Since type A and B subspecies are closely related, we hypothesized that Schu S4 antigens would also have utility for diagnosing type B tularemia caused by strains from other geographic locations. To test this, we probed the Schu S4 array with sera from 241 type B tularemia cases in Spain. Despite there being no type A strains in Spain, we confirmed the responses against some of the same potential serodiagnostic antigens reported previously, as well as determined the responses against additional potential serodiagnostic antigens. Five potential serodiagnostic antigens were evaluated on immunostrips, and two of these (FTT1696/GroEL and FTT0975/conserved hypothetical protein) discriminated between the Spanish tularemia cases and healthy controls. We conclude that antigens from the type A strain Schu S4 are suitable for detection of antibodies from patients with type B F. tularensis infections and that these can be used for the diagnosis of tularemia in a deployable format, such as the immunostrip.T ularemia is a zoonotic disease caused by the Gram-negative facultative anaerobe Francisella tularensis. The organism infects a multitude of different animals, including mammals, birds, fish, and insects. Transmission to humans can occur by inhalation, although the most common natural route is likely to be direct contact with infected animals or via ticks and other biting arthropods that feed on infected mammals. Important animal reservoirs appear to be rodents, hares, and rabbits. For example, the 1997 outbreak in Castilla y León, Spain (1, 2), was transmitted by the handling of infected hares. The following year, another outbreak which was attributed to crayfish handling occurred in Castilla-La Mancha, Spain (3). Human-tohuman transmission is rare.Four closely related subspecies have been defined: F. tularensis subsp. tularensis (type A), F. tularensis subsp. holarctica (type B), F. tularensis subsp. novicida, and F. tularensis subsp. mediasiatica. Each subspecies possesses a different pathogenic potential in humans, as well as a distinctive geographic distribution, host preference, and route of transmission. Type A is found mainly in North America and is associated with severe tularemia that may be fatal, whereas type B is found throughout the Northern Hemisphere and is less virulent in humans (4-6). F. tularensis subsp. ...

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Comparison of Enzyme-Linked Immunosorbent Assay, Western Blotting, Microagglutination, Indirect Immunofluorescence Assay, and Flow Cytometry for Serological Diagnosis of Tularemia

Abstract: The serodiagnostic efficiencies of five different approaches to detecting antibodies (immunoglobulins G,

Cited by 106 publications

References 33 publications

Increase in Human Cases of Tularemia — Colorado, Nebraska, South Dakota, and Wyoming, January–September 2015

Increase in Human Cases of Tularemia — Colorado, Nebraska, South Dakota, and Wyoming, January–September 2015

Current and emerging assays for Francisella tularensis detection: a review

Towards Development of Improved Serodiagnostics for Tularemia by Use of Francisella tularensis Proteome Microarrays

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