Comparison of epithelial and fibroblastic cell behavior on nano/micro-topographic PCL membranes produced by crystallinity control

Gümüşderelı́oğlu, Menemşe; Kaya, Figen; Beşkardeş, Işıl Gerçek

doi:10.1016/j.jcis.2011.03.026

Cited by 28 publications

(30 citation statements)

References 30 publications

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“…It is known that for hydrophobic materials (i. a. PCL) surface roughness enhances the hydrophobicity [39,40]. On the other hand, the water droplet can be spread easier on the more porous surface of membrane, which results in higher hydrophilicity [41].…”

Section: Discussionmentioning

confidence: 99%

Poly(ε-caprolactone)-based membranes with tunable physicochemical, bioactive and osteoinductive properties

Dziadek¹,

Zagrajczuk²,

Menaszek

et al. 2017

J Mater Sci

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In contrast to currently used materials, membranes for the treatment of bone defects should actively promote regeneration of bone tissue beyond their physical barrier function. What is more, both material properties and biological features of membranes should be easily adaptable to meet the needs of particular therapeutic applications. Therefore, the role of preparation methods (nonsolvent-induced phase separation and thermal-induced phase separation) of poly(ε-caprolactone)-based membranes and their modification with gel-derived bioactive glass (BG) particles of two different sizes (\45 and \3 μm) in modulating material morphology, polymer matrix crystallinity, surface wettability, kinetics of in vitro bioactivity and also osteoblast response was investigated. Both surfaces of membranes were characterised in terms of their properties. Our results indicated a possibility to modulate microstructure (pore size ranging from submicron to hundreds of micrometres), wettability (from hydrophobic to fully wettable surface) and polymer crystallinity (from 19 to 60%) in a wide range by the use of various preparation methods and different BG particle sizes. Obtained composite membranes showed excellent in vitro hydroxyapatite forming ability after incubation in simulated body fluid. Here we demonstrated that bioactive layer formation on the surface of membranes occurred through ACP-OCP-CDHA-HCA transformation, that mimic in vivo bone biomineralization process. Composite membranes supported human osteoblast proliferation, stimulated cell differentiation and matrix mineralization. We proved that kinetics of bioactivity process and also osteoinductive properties of membranes can be easily modulated with the use of proposed variables. This brings new

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Section: Discussionmentioning

confidence: 99%

Poly(ε-caprolactone)-based membranes with tunable physicochemical, bioactive and osteoinductive properties

Dziadek¹,

Zagrajczuk²,

Menaszek

et al. 2017

J Mater Sci

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“…Surface roughness plays a vital role for positive cell-material interactions. Some cells, especially fibroblast cells, enjoys rough surfaces and were homogeneously distributed on these surfaces and well spread by elongating themselves [41]. Cellular interaction on a material is an important parameter to determine the biocompatibility or cytotoxicity.…”

Section: Afm and Cell Culturementioning

confidence: 99%

In-situ photocrosslinkable nanohybrid elastomer based on polybutadiene/polyhedral oligomeric silsesquioxane

Mirmohammadi

Nekoomanesh‐Haghighi

Gezaz

et al. 2016

Materials Science and Engineering: C

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Hydroxyl functionalized nano-sized POSS or ethyleneglycol as diol monomers was incorporated to hydroxyl-terminated polybutadiene (HTPBD) chain in the presence of fumaryl chloride as extender. Blue light photocrosslinking system based on camphorquinone (photoinitiator) and dimethylaminoethyl methacrylate (accelerator) was applied to cure these two synthesized fumarate based macromers. Self-crosslinkability of unsaturated macromers and also crosslinking in presence of a reactive diluent were investigated in absence and presence of 1,4-butanediol dimethacrylate, respectively. Finally, photocured samples were characterized by XRD, SEM, equilibrium swelling study, TGA, DMTA, AFM and cell culture. The results showed that incorporation of POSS nanoparticle into the polymer matrix with a perfect distribution and dispersion can enhance thermal stability, mechanical and biocompatibility properties which can prove a good potential of this in-situ photocrosslinkable nanohybrid in medical applications.

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“…Several articles reported contradictory results about the effects of material topography, hydrophobicity and wettability on cell behaviour [19]. Some correlations between the wettability and cell behavior have been reported showing that cells prefer to attach on hydrophilic surfaces.…”

Section: Introductionmentioning

confidence: 99%

Influence of crystallinity and fiber orientation on hydrophobicity and biological response of poly(l-lactide) electrospun mats

et al. 2012

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Royal Society of ChemistryAreias, A.; Ribeiro, C.; Sencadas, V.; Garcia Giralt, N.; Diez Perez, A.; Gómez Ribelles, JL.; Lanceros Mendez, S. (2012). Influence of crystallinity and fiber orientation on hydrophobicity and biological response of poly(L-lactide) electrospun mats. Soft Matter. 8 (21) AbstractPoly(L-lactide) electrospun mates have been produced with random and aligned fiber orientation and degrees of crystallinity from 0 up to nearly 50%. These two factors, fiber alignment and degree of crystallinity strongly affect the hydrophobicity of the samples, being this larger for the aligned fiber mats and for the fibers with higher degree of crystallinity. Whereas the first effect can be associated to a decrease in the degree of porosity the second should be related to variations in the fiber roughness at nanometric scale and an increase in fiber stiffness. Proliferation of human chondrocytes cultured in monolayer on these substrates is similar in both aligned and non-aligned amorphous mats. Crystallization of the aligned mats, on the other hand, nearly suppresses proliferation and the cells produce higher amounts of aggrecan, characteristic of the extracellular matrix of hyaline cartilage.

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Comparison of epithelial and fibroblastic cell behavior on nano/micro-topographic PCL membranes produced by crystallinity control

Cited by 28 publications

References 30 publications

Poly(ε-caprolactone)-based membranes with tunable physicochemical, bioactive and osteoinductive properties

Poly(ε-caprolactone)-based membranes with tunable physicochemical, bioactive and osteoinductive properties

In-situ photocrosslinkable nanohybrid elastomer based on polybutadiene/polyhedral oligomeric silsesquioxane

Influence of crystallinity and fiber orientation on hydrophobicity and biological response of poly(l-lactide) electrospun mats

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