Comparison of estrogen-responsive plasma protein biomarkers and reproductive endpoints in sheepshead minnows exposed to 17β-trenbolone

Hemmer, Michael J.; Cripe, Geraldine M.; Hemmer, Becky L.; Goodman, Larry R.; Salinas, Kimberly A.; Fournie, John W.; Walker, Calvin C

doi:10.1016/j.aquatox.2008.04.001

Cited by 26 publications

(27 citation statements)

References 52 publications

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“…Vitellogenin in female fish generally decreases with increasing concentrations of Tb [6,9,10]. Although F1 VTG appeared similar in all treatments, it did not prevent a virtual collapse of reproduction at 0.87 mg Tb/L.…”

Section: Discussionmentioning

confidence: 57%

“…Treatment concentrations were chosen based on the results of a 21-d exposure [10]. Nominal exposure concentrations were: seawater control, solvent control, 0.01, 0.04, 0.2, 1.0, and 5.0 mg Tb/L; exposure water was sampled weekly in all aquaria with live fish.…”

Section: Test Chemical and Analysesmentioning

confidence: 99%

“…This persistence creates particular concern for species living in waters receiving these metabolites, because population sustainability may be at risk as a result of altered mating behavior [6], reproduction [10], and development [9]. Surface drainage waters near a cattle feedlot produced elevated androgenic activity as measured by a cellbased androgen receptor assay and contained metabolites of trenbolone acetate [14,15].…”

Section: Introductionmentioning

confidence: 99%

“…To date, most androgen effects on fish have been evaluated using short-term ( 21-d) trenbolone exposures measuring fecundity, gene expression, morphology, gonadosomatic index, vitellogenin and plasma steroid changes, and protein biomarkers [6][7][8][9][10]. An exception is the study by Seki et al [11], in which medaka (Oryzias latipes) exposed to the androgen, methyl testosterone, through a full life cycle to 60-d posthatch (dph) of the F1 generation had altered secondary sex characteristics (F0), gonadal histology (F0), and hepatic vitellogenin levels (F1).…”

Section: Introductionmentioning

confidence: 99%

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Exposure of three generations of the estuarine sheepshead minnow (Cyprinodon variegatus) to the androgen, 17β‐trenbolone: Effects on survival, development, and reproduction

Cripe

Hemmer

Raimondo

et al. 2010

Enviro Toxic and Chemistry

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Estimating long-term effects of endocrine-disrupting chemicals on a species is important to assessing the overall risk to the populations. The present study reports the results of a 42-week exposure of estuarine sheepshead minnows (Cyprinodon variegatus) to the androgen, 17beta-trenbolone (Tb) conducted to determine if partial-(F0) or single-generation (F1) fish exposures identify multigenerational (F0-F3) effects of androgens on fish. Adult F0 fish were exposed to 0.007, 0.027, 0.13, 0.87,and 4.1 microg Tb/L, the F1 generation to < or =0.87 microg Tb/L, the F2 fish to < or =0.13 microg Tb/L, and the F3 fish to < or =0.027 microg Tb/L. The highest concentrations with reproducing populations at the end of the F0, F1, and F2 generations were 4.1, 0.87, and 0.027 microg Tb/L, respectively. Reproduction in the F0, F1, and F2 generations was significantly reduced at 0.87, 0.027, and 0.027 microg Tb/L, respectively. Fish were significantly masculinized in the F1 generation exposed to 0.13 microg Tb/L or greater. Female plasma vitellogenin was significantly reduced in F0 fish exposed to > or =0.87 microg Tb/L. Gonadosomatic indices of the F0 and F1 generations were significantly increased at 0.87 and 0.13 microg Tb/L in the F0 and F1 generation, respectively, and were accompanied by ovarian histological changes. Reproduction was the most consistently sensitive measure of androgen effects and, after a life-cycle exposure, the daily reproductive rate predicted concentrations affecting successive generations. The present study provides evidence that a multiple generation exposure of fish to some endocrine-disrupting chemicals can result in developmental and reproductive changes that have a much greater impact on the success of a species than was indicated from shorter term exposures.

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Section: Discussionmentioning

confidence: 57%

Section: Test Chemical and Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exposure of three generations of the estuarine sheepshead minnow (Cyprinodon variegatus) to the androgen, 17β‐trenbolone: Effects on survival, development, and reproduction

Cripe

Hemmer

Raimondo

et al. 2010

Enviro Toxic and Chemistry

Self Cite

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“…We were unable to measure circulating estrogens in our study, since the currently available methods lack the sensitivity required for this analysis in orchiectomized male rodents. However, in vitro evidence indicates that trenbolone and its primary metabolites are relatively nonestrogenic (30), and in vivo evidence demonstrates that trenbolone induces antiestrogenic effects in oviparous species (3,16,23,32,38,53). Additionally, it has been reported that trenbolone does not undergo aromatization due to its 3-oxotriene structure (46), although conflicting reports exist regarding the influence of trenbolone on circulating estrogens in mammalian species (24,26).…”

Section: E657mentioning

confidence: 99%

17β-Hydroxyestra-4,9,11-trien-3-one (trenbolone) exhibits tissue selective anabolic activity: effects on muscle, bone, adiposity, hemoglobin, and prostate

Yarrow

Conover

McCoy

et al. 2011

American Journal of Physiology-Endocrinology and Metabolism

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Yarrow JF, Conover CF, McCoy SC, Lipinska JA, Santillana CA, Hance JM, Cannady DF, VanPelt TD, Sanchez J, Conrad BP, Pingel JE, Wronski TJ, Borst SE. 17␤-Hydroxyestra-4,9,11-trien-3-one (trenbolone) exhibits tissue selective anabolic activity: effects on muscle, bone, adiposity, hemoglobin, and prostate. Am J Physiol Endocrinol Metab 300: E650 -E660, 2011; First published January 25, 2011; doi:10.1152/ajpendo.00440.2010.-Selective androgen receptor modulators (SARMs) now under development can protect against muscle and bone loss without causing prostate growth or polycythemia. 17␤-Hydroxyestra-4,9,11-trien-3-one (trenbolone), a potent testosterone analog, may have SARM-like actions because, unlike testosterone, trenbolone does not undergo tissue-specific 5␣-reduction to form more potent androgens. We tested the hypothesis that trenbolone-enanthate (TREN) might prevent orchiectomy-induced losses in muscle and bone and visceral fat accumulation without increasing prostate mass or resulting in adverse hemoglobin elevations. Male F344 rats aged 3 mo underwent orchiectomy or remained intact and were administered graded doses of TREN, supraphysiological testosterone-enanthate, or vehicle for 29 days. In both intact and orchiectomized animals, all TREN doses and supraphysiological testosterone-enanthate augmented androgen-sensitive levator ani/ bulbocavernosus muscle mass by 35-40% above shams (P Յ 0.001) and produced a dose-dependent partial protection against orchiectomy-induced total and trabecular bone mineral density losses (P Ͻ 0.05) and visceral fat accumulation (P Ͻ 0.05). The lowest doses of TREN successfully maintained prostate mass and hemoglobin concentrations at sham levels in both intact and orchiectomized animals, whereas supraphysiological testosteroneenanthate and high-dose TREN elevated prostate mass by 84 and 68%, respectively (P Ͻ 0.01). In summary, low-dose administration of the non-5␣-reducible androgen TREN maintains prostate mass and hemoglobin concentrations near the level of shams while producing potent myotrophic actions in skeletal muscle and partial protection against orchiectomy-induced bone loss and visceral fat accumulation. Our findings indicate that TREN has advantages over supraphysiological testosterone and supports the need for future preclinical studies examining the viability of TREN as an option for androgen replacement therapy.

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Mummichog (Fundulus heteroclitus) continue to successfully produce eggs after exposure to high levels of 17α‐ethinylestradiol

Bosker

Munkittrick

Lister

et al. 2016

Enviro Toxic and Chemistry

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17α-Ethinylestradiol (EE2) is a potent estrogen used in birth-control pills. Previous laboratory and field studies have shown negative impacts in a variety of fish species after exposure to low levels of EE2, most notably a nearly complete shutdown of egg production. The present study demonstrates that mummichog (Fundulus heteroclitus), a small-bodied estuarine species, is able to continue to produce eggs after exposure for 28 d to 100 ng of EE2/L. No effect of EE2 on egg production was observed, whereas a >35-fold increase in vitellogenin (vtg 1) gene expression in males was found. The lack of response in egg production in fish exposed to high levels of EE2 warrants further investigations on species-specific responses to estrogens and endocrine disruptors in general.

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Comparison of estrogen-responsive plasma protein biomarkers and reproductive endpoints in sheepshead minnows exposed to 17β-trenbolone

Cited by 26 publications

References 52 publications

Exposure of three generations of the estuarine sheepshead minnow (Cyprinodon variegatus) to the androgen, 17β‐trenbolone: Effects on survival, development, and reproduction

Exposure of three generations of the estuarine sheepshead minnow (Cyprinodon variegatus) to the androgen, 17β‐trenbolone: Effects on survival, development, and reproduction

17β-Hydroxyestra-4,9,11-trien-3-one (trenbolone) exhibits tissue selective anabolic activity: effects on muscle, bone, adiposity, hemoglobin, and prostate

Mummichog (Fundulus heteroclitus) continue to successfully produce eggs after exposure to high levels of 17α‐ethinylestradiol

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