Comparison of ESTs from juvenile and adult phases of the giant unicellular green alga Acetabularia acetabulum

Henry, Isabelle; Wilkinson, Mark D.; Hernandez, J. Marcela; Schwarz‐Sommer, Zsuzsanna; Grotewold, Erich; Mandoli, Dina F.

doi:10.1186/1471-2229-4-3

Cited by 28 publications

(3 citation statements)

References 44 publications

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“…Moreover, algae are generally far more diverse and evolutionarily divergent than land plants [35]. Currently, nearly complete genomic information is available publicly for only five green microalgae species: Chlamydomonas reinhardtti (Chlorophyceae) [36], Chlorella variabilis (Trebouxiophyceae) [37], Ostreococcus tauri (Prasinophyceae) [38], Micromonas (Prasinophyceae) [39] and Volvox carteri (Chlorophyceae) [40].…”

Section: Introductionmentioning

confidence: 99%

“…To date, there is little genomic data available for the Ulvophyceae, a group that is diverse and evolutionarily divergent from all of the currently sequenced genomes of green microalgae. For the Ulvophyceae group, EST resources have been developed using Sanger sequencing in three species, Acetabularia acetabulum [35], Ulva linza [41] and Ulva prolifera [42] . A comprehensive description of the full complement of genes as a transcriptome, however, is still unavailable.…”

Section: Introductionmentioning

confidence: 99%

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De novo sequencing and analysis of the Ulva linza transcriptome to discover putative mechanisms associated with its successful colonization of coastal ecosystems

Zhang

Liang

et al. 2012

BMC Genomics

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BackgroundThe green algal genus Ulva Linnaeus (Ulvaceae, Ulvales, Chlorophyta) is well known for its wide distribution in marine, freshwater, and brackish environments throughout the world. The Ulva species are also highly tolerant of variations in salinity, temperature, and irradiance and are the main cause of green tides, which can have deleterious ecological effects. However, limited genomic information is currently available in this non-model and ecologically important species. Ulva linza is a species that inhabits bedrock in the mid to low intertidal zone, and it is a major contributor to biofouling. Here, we presented the global characterization of the U. linza transcriptome using the Roche GS FLX Titanium platform, with the aim of uncovering the genomic mechanisms underlying rapid and successful colonization of the coastal ecosystems.ResultsDe novo assembly of 382,884 reads generated 13,426 contigs with an average length of 1,000 bases. Contiguous sequences were further assembled into 10,784 isotigs with an average length of 1,515 bases. A total of 304,101 reads were nominally identified by BLAST; 4,368 isotigs were functionally annotated with 13,550 GO terms, and 2,404 isotigs having enzyme commission (EC) numbers were assigned to 262 KEGG pathways. When compared with four other full sequenced green algae, 3,457 unique isotigs were found in U. linza and 18 conserved in land plants. In addition, a specific photoprotective mechanism based on both LhcSR and PsbS proteins and a C4-like carbon-concentrating mechanism were found, which may help U. linza survive stress conditions. At least 19 transporters for essential inorganic nutrients (i.e., nitrogen, phosphorus, and sulphur) were responsible for its ability to take up inorganic nutrients, and at least 25 eukaryotic cytochrome P450s, which is a higher number than that found in other algae, may be related to their strong allelopathy. Multi-origination of the stress related proteins, such as glutamate dehydrogenase, superoxide dismutases, ascorbate peroxidase, catalase and heat-shock proteins, may also contribute to colonization of U. linza under stress conditions.ConclusionsThe transcriptome of U. linza uncovers some potential genomic mechanisms that might explain its ability to rapidly and successfully colonize coastal ecosystems, including the land-specific genes; special photoprotective mechanism based on both LhcSR and PsbS; development of C4-like carbon-concentrating mechanisms; muti-origin transporters for essential inorganic nutrients; multiple and complex P450s; and glutamate dehydrogenase, superoxide dismutases, ascorbate peroxidase, catalase, and heat-shock proteins that are related to stress resistance.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

De novo sequencing and analysis of the Ulva linza transcriptome to discover putative mechanisms associated with its successful colonization of coastal ecosystems

Zhang

Liang

et al. 2012

BMC Genomics

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“…It comprised of three anatomical parts: at the bottoma rhizoid with a set of short roots, in the middlea long stalk, and at the topan umbrella of branches that further fused into a cap. It is also called -Mermaid's Wineglass‖ because of its beautiful umbrella-shaped cap 5 . The life cycle of Acetabularia is complex and needs approximately 115 days to complete 6 .…”

Section: Introductionmentioning

confidence: 99%

Morpho-molecular assessment of Acetabularia jalakanyakae Sp. Nov. (Dasycladales, Chlorophyta) - a new species from Andaman and Nicobar Islands, India

2021

IJMS

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Acetabularia (Dasycladales) is an extant genus of a single-celled green alga. There are four species of this genus reported from India, three reported from Andaman and Nicobar Islands. For this study, Acetabularia isolate was collected from a rocky intertidal habitat in the Andaman and Nicobar Islands. Light microscopy and Scanning Electron Microscopy were used for the morphological characterization. The distinct traits of caps of the thalli were prioritized because, traditionally, species delimitations in Acetabularia mainly were based on cap morphology. Our isolate showed morphological similarity with Acetabularia crenulata. However, the number of hairs in the inner ring of lobes of caps and the stalk length were observed to be different from A. crenulata and other closely related species. The phylogenetic tree constructed for partial 18S rDNA using the Maximum Likelihood (ML) method revealed the evolutionary affinity of this new species with Acetabularia dentata. Based on morphological and molecular synapomorphy, a new species of Acetabularia, Acetabularia jalakanyakae is formally proposed herein, and the further implications of this species discovery are discussed.

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Genetic Technologies and Enhancement of Algal Utilization in Wastewater Treatment and Bioremediation

El‐Esawi

2019

Application of Microalgae in Wastewater Treatment

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Comparison of ESTs from juvenile and adult phases of the giant unicellular green alga Acetabularia acetabulum

Cited by 28 publications

References 44 publications

De novo sequencing and analysis of the Ulva linza transcriptome to discover putative mechanisms associated with its successful colonization of coastal ecosystems

De novo sequencing and analysis of the Ulva linza transcriptome to discover putative mechanisms associated with its successful colonization of coastal ecosystems

Morpho-molecular assessment of Acetabularia jalakanyakae Sp. Nov. (Dasycladales, Chlorophyta) - a new species from Andaman and Nicobar Islands, India

Genetic Technologies and Enhancement of Algal Utilization in Wastewater Treatment and Bioremediation

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