Comparison of Ethanol Extract from Roots, Leaves, and Flowers of Calotropis gigantea as Anticancer on T47D Breast Cancer Cell Lines

Mutiah, Roihatul; Sukardiman, Sukardiman; Widyawaruyanti, Aty; Zulaikah, Siti

doi:10.18860/al.v5i1.3690

Cited by 7 publications

(11 citation statements)

References 5 publications

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“…As reported by the researchers in a previous study, C. gigantea leaf ethanol extract can inhibit the growth of in vivo fibrosarcoma at doses of 100 and 150 mg/kg with the mechanism of increased expression of caspase-3 (Mutiah et al, 2016). Ethanol extract of the roots and C. gigantea leaves have an anticancer activity which is higher than the flower (Mutiah et al, 2016). Ethyl acetate fraction from the leaves (IC 50 41, 79 µg/mL) and dichloromethane fraction (IC 50 40.57 μg/mL) have cytotoxic activity which is higher than butanol fraction (IC 50 737.74 ug/mL) and water (IC 50 8493 ug/mL) (Mutiah et al, 2017).…”

Section: Introductionsupporting

confidence: 56%

Section: Introductionmentioning

confidence: 56%

“…Leaf extract of Calotropis gigantea (EDCG) has been reported of its ability in impeding the growth of cancer cell through apoptosis induction by raising the three caspase expression (Mutiah, 2016). While the 5 Fluorouracil is a chemotherapy agent with a working mechanism through improving the expressions of p21 and pRb without involving the role of p53.…”

Section: Discussionmentioning

confidence: 99%

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Calotropis gigantea Leaf Extract Increases the Efficacy of 5-Fluorouracil and Decreases the Efficacy of Doxorubicin in Widr Colon Cancer Cell Culture

2018

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Colon cancer is a malignant neoplasm with high incidence and causes the death of more than 30% of the patients. Although there have been efforts to increase the life of the patient using chemotherapy agents, unspecified targets of drugs cause serious side effects and lead to multiple drug resistance (MDR). This is a major problem in cancer therapy in general. Efforts to use substances from plants that have low toxicity give new hope as a co-chemotherapy agent that can increase the efficacy of chemotherapy agents and reduce their toxicity to normal cells. This study aimed to determine the synergistic effects of therapy combination of Calotropis gigantea (EDCG) leaf extract with the chemotherapy drug 5-Fluorouracil (5-FU) and doxorubicin against WiDR colon cancer cells. The analysis results of MTT showed that the combination of EDCG and 5-Fluorouracil gives synergism effect at the dose combination of EDCG+5FU (3 µg/ml + 62.5 nM; 3 µg/ml + 125 nM; 3 µg/ml + 250 nM). While the combination of doxorubicin and EDCG creates antagonistic effects of mild to the strong antagonist, EDCG may enhance the efficacy of 5 Fluorouracil but decrease the efficacy of doxorubicin. Therefore, a combination of EDCG and 5-Fluorouracil may be recommended for further study.

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Section: Introductionsupporting

confidence: 56%

Section: Introductionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

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Calotropis gigantea Leaf Extract Increases the Efficacy of 5-Fluorouracil and Decreases the Efficacy of Doxorubicin in Widr Colon Cancer Cell Culture

2018

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“…After cell viability is known, a combination index is calculated. Combination index is a standard method used to evaluate drug combinations [14]. The smaller the value of a combination index, the more synergistic the combination will be -the results of the combination index calculation presented in Table 1.…”

Section: Anticancer Activity Combination Of Doxorubicin and E Palmifolia Extractmentioning

confidence: 99%

Combinational effect of Eleutherine palmifolia (L.) merr extract and doxorubicin chemotherapy on HeLa cervical cancer cells

Mutiah

Choiroh

Annisa

et al. 2019

AIP Conference Proceedings

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Eleutherine palmifolia (L.) Merr (E. palmifolia) is a typical Central Borneo plant that empirically has many benefits in the health sector. E. palmifolia is known to have flavonoids which have anticancer activities. Cancer is the leading cause of death throughout the world with the most cancer cases, namely breast and cervical cancer. One of the reasons for cancer failure is due to resistance to the use of chemotherapy drugs. One way to overcome to combine chemotherapy agents with chemoprevention agents from natural products. This study aims to evaluate the potential combination of doxorubicin and extract E. palmifolia on anticancer activity and HeLa cell apoptosis induction. Single anticancer activities and combinations were determined by using the MTT method. The effect of the combination on apoptosis induction known by the flow cytometry method. The results showed that the anticancer activity of the best combination obtained by a combination of 50 ppm doxorubicin and extract E. palmifolia 100 ppm. The apoptosis test results showed that the best combination of doxorubicin and extract E. palmifolia could not increase the apoptosis of HeLa cervical cancer cells.

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“…Pada penelitian sebelumnya telah dilaporkan bahwa senyawa calotropon dari bagian akar mempunyai aktivitas sitotoksik terhadap Leukemia K562 dan gastric cancer 7901 [5]; ekstrak etanol daun C.gigantea mampu menghambat pertumbuhan fibrosarcoma secara in vivo pada dosis 100 dan 150 mg/kgbb dengan mekanisme peningkatan ekspresi caspase-3 [6]. Ekstrak akar C.gigantea mempunyai aktivitas antikanker yang lebih tinggi dibanding bagian daun dan bagian bunga [7]. Fraksi etil asetat dari bagian daun (IC50 41.79 μg/ml) dan fraksi diklorometan (IC5040.57μg/ml) mempunyai aktivitas sitotoksik yang lebih tinggi dibanding fraksi butanol (IC50 737.74 μg/ml) dan air (IC50 8493 μg/ml) [8].…”

Section: Pendahuluanunclassified

Studi Efikasi dan Keamanan Ekstrak Akar dan Daun Calotropis gigantea Terhadap Sel Kanker Kolon dan Sel Kanker Payudara Secara In Vitro

Mutiah

2017

JIM

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ABSTRAKPengembangan fitofarmakauntuk penyakit kanker sampai saat ini menjadi langkah utama dalam mengatasi kegagalan terapi kanker. Calotropis gigantea adalah salah satu tanaman yang telah terbukti secara ilmiah baik in vitro maupun in vivo sebagai agen kemopreventif. Penelitian ini bertujuan untuk menjelaskan efikasi dan keamanan ekstrak daun Calotropis gigantea (EDCG) dan ekstrak akar Calotropis gigantea (EACG) pada sel normal NIH3T3, sel kanker kolon WiDr dan sel kanker payudara T47D. Dari hasil analisis MTT dan analisis Selectivity Index (SI) didapatkan hasil bahwa Ekstrak etanol daun Calotropis gigantea (EDCG) dapat menghambat pertumbuhan sel kanker kolon WiDr secara selektif (SI>3) namun tidak selektif dalam menghambat pertumbuhan sel kanker payudara T47D (SI<3). Ekstrak etanol akar Calotropis gigantea (EACG) tidak selektif dalam menghambat pertumbuhan sel kanker kolon WiDr dan sel kanker payudara T47D (SI<3). Oleh karena itu EDCG dapat direkomendasikan sebagai kandidat Fitofarmaka dengan pembuktian lebih lajut melalui uji klinik.Katakunci : Calotropis gigantea, Sel NIH3T3, Sel WiDr, Sel T47D, efikasi, keamanan, Selectivity Index (SI) ABSTRACT Development of phytopharmaca for cancer until now has become a major step in overcoming the failure of cancer therapy. Calotrophis gigantea is one of the scientifically proven plants both in vitro and in vivo as a chemopreventive agent. This study aims to explain the efficacy and safety of Calotropis gigantea leaf extract (EDCG) and Calotropis gigantea root extract (EACG) on NIH3T3 cell, colon cancer cell WiDr and T47D breast cancer cells. From the analysis of MTT and Selectivity Index (SI) analysis showed that ethanol extract of leaf Calotropis gigantea (EDCG) can inhibit selective growth of selective colon cancer cells (SI> 3) but not selective in inhibiting the growth of breast cancer cells T47D (SI <3 ). Calotropic gigantea (EACG) root ethanol extract is not selective in inhibiting the growth of colon cancer cells WiDr and breast cancer cells T47D (SI <3). Therefore EDCG can be recommended as a Phytopharmaca candidate with further proof through clinical trials.

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Comparison of Ethanol Extract from Roots, Leaves, and Flowers of Calotropis gigantea as Anticancer on T47D Breast Cancer Cell Lines

Cited by 7 publications

References 5 publications

Calotropis gigantea Leaf Extract Increases the Efficacy of 5-Fluorouracil and Decreases the Efficacy of Doxorubicin in Widr Colon Cancer Cell Culture

Calotropis gigantea Leaf Extract Increases the Efficacy of 5-Fluorouracil and Decreases the Efficacy of Doxorubicin in Widr Colon Cancer Cell Culture

Combinational effect of Eleutherine palmifolia (L.) merr extract and doxorubicin chemotherapy on HeLa cervical cancer cells

Studi Efikasi dan Keamanan Ekstrak Akar dan Daun Calotropis gigantea Terhadap Sel Kanker Kolon dan Sel Kanker Payudara Secara In Vitro

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