The grid-generated turbulence in wind tunnel is commonly applied in aerodynamic performance experiments of various industrial structures. However, the characteristics and application conditions of the grid-generated turbulence in the wind tunnel are often affected by the grid shape and the test position, respectively. Therefore, in order to clarify the influence of the grid shape and the test position on the grid-generated turbulence in wind tunnel, a computational fluid dynamics (CFD) numerical study on the turbulence generated by two different grids was presented herein, with the wind tunnel experimental validation. Firstly, time histories of the wind speed at 19 monitoring points behind the grid in wind tunnel were collected. Secondly, the entire turbulent field generated by two different grids was simulated using CFD. Then, the characteristics of the turbulence generated by the grids in wind tunnel were investigated. Finally, the accuracy of the CFD simulations compared with the wind tunnel test was studied. Results show that the grid blockage and the interference effect lead to a significant increase of 200% in the turbulence intensity near the grid. Moreover, the grid-generated turbulence conforms to the isotropic assumption only after a specific distance of 2.0-4.0 m from the grid. In addition, CFD errors are generally within 15%-20% of the corresponding measurements in the wind tunnel. This study provides guidance for selecting the installation location of the structure model in a wind tunnel.